The binding of indocyanine green and other organic anions to serum proteins in liver diseases.

Abstract Using Sephadex G-200 column chromatography, the binding patterns of organic anions with serum proteins were studied in normal subjects and in patients with liver diseases. BSP, [131I]BSP and [131I] Rose Bengal were bound mainly to albumin while small amounts were bound to globulin fractions. In normal sera, indocyanine green (ICG) in lower concentrations was bound mainly to β-lipoprotein while in higher concentrations it was bound to all fractions of proteins. In the various liver diseases, the binding patterns of ICG showed characteristic changes in comparison to those other organic anions. In acute hepatitis, obstructive jaundice and the Dubin-Johnson syndrome, the characteristic ICG peak II was decreased. In chronic liver diseases, such as chronic hepatitis and liver cirrhosis, the ICG peak II was increased remarkably and was bound to heavier proteins. Using single radial immunodiffusion and autoradiography, the binding of [131I]BSP with normal sera were studied. [131I] BSP was mainly to albumin, prealbumin and β-lipoprotein while small amounts were bound to the globulin fractions.