Crystallization of a protein using dehydration without a precipitant.

Hypoxic response protein I (HRPI) is a protein of unknown biochemical function whose expression is very strongly upregulated in response to oxygen depletion in Mycobacterium tuberculosis. Crystals have been grown from a solution of full-length HRPI by the unusual method of dehydration without the use of precipitants. The crystals produced diffract to a maximum resolution of 2.1 A and belong to space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 79.18, c = 37.34 A.

[1]  Martin Tompa,et al.  Rv3133c/dosR is a transcription factor that mediates the hypoxic response of Mycobacterium tuberculosis , 2003, Molecular microbiology.

[2]  S. Arend,et al.  Evidence of endogenous reactivation of tuberculosis after a long period of latency. , 2002, The Journal of infectious diseases.

[3]  Richard A. Slayden,et al.  Hypoxic Response of Mycobacterium tuberculosis Studied by Metabolic Labeling and Proteome Analysis of Cellular and Extracellular Proteins , 2002, Journal of bacteriology.

[4]  A. Dirksen,et al.  Molecular evidence of endogenous reactivation of Mycobacterium tuberculosis after 33 years of latent infection. , 2002, The Journal of infectious diseases.

[5]  T. Dick,et al.  Proteins of Mycobacterium bovis BCG Induced in the Wayne Dormancy Model , 2001, Journal of bacteriology.

[6]  C. Dye,et al.  Consensus statement. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project. , 1999, JAMA.

[7]  A. Vagin,et al.  MOLREP: an Automated Program for Molecular Replacement , 1997 .

[8]  L. Wayne,et al.  An in vitro model for sequential study of shiftdown of Mycobacterium tuberculosis through two stages of nonreplicating persistence , 1996, Infection and immunity.

[9]  J. Navaza,et al.  AMoRe: an automated package for molecular replacement , 1994 .

[10]  G. Nemerow,et al.  Strategies in the crystallization of glycoproteins and protein complexes , 1992 .

[11]  Lorenz Wernisch,et al.  The influence of reduced oxygen availability on pathogenicity and gene expression in Mycobacterium tuberculosis. , 2004, Tuberculosis.

[12]  G. Schoolnik,et al.  Mycobacterium tuberculosis gene expression during adaptation to stationary phase and low-oxygen dormancy. , 2004, Tuberculosis.

[13]  Tanya Parish,et al.  Gene expression profile of Mycobacterium tuberculosis in a non-replicating state. , 2004, Tuberculosis.

[14]  T. Yeates,et al.  [22] Detecting and overcoming crystal twinning. , 1997, Methods in enzymology.