Evidence for the establishment of aphid-eubacterium endosymbiosis in an ancestor of four aphid families

Aphids (superfamily Aphidoidea) contain eubacterial endosymbionts localized within specialized cells (mycetocytes). The endosymbionts are essential for the survival of the aphid hosts. Sequence analyses of the 16S rRNAs from endosymbionts of 11 aphid species from seven tribes and four families have indicated that the endosymbionts are monophyletic. Furthermore, phylogenetic relationships within the symbiont clade parallel the relationships of the corresponding aphid hosts. Our findings suggest that this endocytobiotic association was established in a common ancestor of the four aphid families with subsequent diversification into the present species of aphids and their endosymbionts.

[1]  D. Ord,et al.  PAUP:Phylogenetic analysis using parsi-mony , 1993 .

[2]  M. Munson,et al.  Buchnera gen. nov. and Buchnera aphidicola sp. nov., a Taxon Consisting of the Mycetocyte-Associated, Primary Endosymbionts of Aphids , 1991 .

[3]  J. Neefs,et al.  Compilation of small ribosomal subunit RNA sequences. , 1991, Nucleic acids research.

[4]  M. Riley,et al.  Organization of the bacterial chromosome , 1990, Microbiological reviews.

[5]  R. Campbell,et al.  Aphid-Plant Genotype Interactions , 1990 .

[6]  M. Donoghue,et al.  PATTERNS OF VARIATION IN LEVELS OF HOMOPLASY , 1989, Evolution; international journal of organic evolution.

[7]  A. Douglas,et al.  MYCETOCYTE SYMBIOSIS IN INSECTS , 1989, Biological reviews of the Cambridge Philosophical Society.

[8]  P. Baumann,et al.  Pea aphid symbiont relationships established by analysis of 16S rRNAs , 1989, Journal of bacteriology.

[9]  A. Dixon,et al.  A rapid method to isolate symbiotic bacteria from aphids , 1989 .

[10]  J. Johnson,et al.  EVOLUTIONARY INFERENCES FROM A COMPARISON OF COCKROACH NUCLEAR DNA AND DNA FROM THEIR FAT‐BODY AND EGG ENDOSYMBIONTS , 1989, Evolution; international journal of organic evolution.

[11]  H. Ishikawa,et al.  Biochemical and molecular aspects of endosymbiosis in insects. , 1989, International review of cytology.

[12]  C. Woese,et al.  Bacterial evolution , 1987, Microbiological reviews.

[13]  A. Dixon,et al.  The mycetocyte symbiosis of aphids: Variation with age and morph in virginoparae of Megoura viciae and Acyrthosiphon pisum , 1987 .

[14]  A. K. Minks,et al.  Aphids. Their biology, natural enemies and control. Volume 2A. , 1987 .

[15]  A. K. Minks,et al.  Paleontology and phylogeny , 1987 .

[16]  D. Weeks,et al.  A small-scale five-hour procedure for isolating multiple samples of CsCl-purified DNA: application to isolations from mammalian, insect, higher plant, algal, yeast, and bacterial sources. , 1986, Analytical biochemistry.

[17]  N. Pace,et al.  Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Thomas A. Kunkel,et al.  Rapid and efficient site-specific mutagenesis without phenotypic selection. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[19]  M. Llewellyn.  The biology of aphids , 1984 .

[20]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[21]  O. Heie.  The Aphidoidea (Hemiptera) of Fennoscandia and Denmark, Volume 1. General Part. The Families Mindaridae, Hormaphididae, Thelaxidae, Anoeciidae, and Pemphigidae , 1980 .

[22]  G. Griffiths,et al.  Intracellular Symbiotes of the Homoptera , 1980 .

[23]  P. Srivastava,et al.  Nucleic acid, nucleotide and protein concentrations in the pea aphid, Acyrthosiphon pisum, during larval growth and development. , 1980 .

[24]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[25]  E. J. Houk,et al.  Phase contrast and electron microscopy of the mycetocytes and symbiotes of the pea aphid, Acyrthosiphon pisum , 1973 .

[26]  P. Buchner.  Endosymbiosis of Animals with Plant Microorganisms , 1965 .

[27]  C. Hogben.  Alimentary Tract , 2004, Abdominal Imaging.