The use of chia seeds as food is increasing as well as its culture, which leads to the need for quality monitoring of seeds and the establishment of standards for such, non-existent in Brazil. This paper evaluates the effect of two temperatures (18 and 25 °C) and three substrates (between-paper, on-paper and paper-roll) on chia seed germination. A completely randomized design in 2 x 3 factorial arrangement with four replications of 100 seeds was carried out. Each replicate was individually wrapped in gearbox box kept in chambers of BOD (18 °C) and Mangelsdorf (25 °C). The evaluation of the percentage of germination was performed on 7 and 14 days by counting the germinated seeds. There was no interaction between factors and the temperature of 25 °C showed better results for the germination of chia seeds (95%), regardless of substrates. The paper-between substrate performed best showing 84% germination at 18 °C. Additional keywords: Salvia hispanica; seeds quality; viability. Resumo O uso das sementes de chia na alimentacao esta aumentando, assim como seu cultivo, que leva a necessidade de monitoramento de qualidade de sementes bem como ao estabelecimento de normas para tal, inexistentes no Brasil. Objetivou-se com o presente trabalho avaliar o efeito de duas temperaturas (18 e 25 °C) e tres substratos (entre papel, sobre papel e rolo de papel) em testes de germinacao de sementes de chia. Adotou-se o delineamento inteiramente casualizado, em esquema fatorial (2 × 3), com quatro repeticoes de 100 sementes. As repeticoes foram acondicionadas em câmara do tipo BOD (18 °C) e germinadores Mangelsdorf (25 °C). A avaliacao da percentagem de germinacao foi realizada aos 7 e aos 14 dias, mediante a contagem das sementes germinadas. Houve interacao entre os fatores, sendo que a temperatura de 25 °C apresentou melhores resultados para a germinacao das sementes de chia (95%), independentemente dos substratos. Com a temperatura de 18 °C, o substrato entre papel teve o melhor desempenho, apresentando 84% de germinacao.
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