Non‐radioactive SSCP for genotyping human platelet alloantigens

Human platelet alloantigen systems are responsible for neonatal and post‐transfusional thrombocytopenias. The determination of the different allotypes can be performed using immunological or DNA‐based methods. The most used DNA‐based procedure requires the digestion by specific restriction enzymes of PCR products containing the genetic determinants of these alloantigens. We now report a rapid method of genotyping which does not use restriction enzymes and is less prone to misinterpretation. This is non‐radioactive PCR‐SSCP (single strand conformation polymorphism), which we illustrate for two different HPA system, one on GPIIIa (HPA‐1) and the other on GPIIb (HPA‐3).

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