Isolation of berberine from Berberis vulgaris Linn. and standardization of aqueous extract by RP-HPLC.

Berberis vulgaris L. belongs to family Berberidaceae is native to Europe and the British Isles in Iran. Barberries is an important production of South Khorasan; biggest producer of barberries in Iran. It is a deciduous shrub having yellow flowers and scarlet colored fruit in the form of berries. Twenty two alkaloids have been reported so far from root, stem leaves and fruit of this plant, which are of medicinal importance. From preliminary Phytochemical analysis showed the presence of carbohydrate, glycoside, alkaloid, protein, amino acid, saponin, tannin and flavonoid. One of the major Isoquinoline alkaloid is Berberine. From the present investigation an attempt has been made to standardize aqueous extract of Berberis vulgaris on the basis %age Berberine content by RP-HPLC.

[1]  V. Joshi,et al.  Some Plants used in Ayurvedic and Homoeopathic Medicine , 2013 .

[2]  B. Ranjan,et al.  Standardization and phytochemical investigation of Berberis aristata , 2012 .

[3]  D. Patel,et al.  Standardization of Berberis aristata extract through conventional and modern HPTLC techniques , 2012 .

[4]  S. M. Javadzadeh,et al.  THERAPEUTIC APPLICATIONOF DIFFERENT PARTS BERBERIS VULGARIS , 2012 .

[5]  M. Golzarand,et al.  EFFECT OF PROCESSED BERBERIS VULGARIS IN APPLE VINEGAR ON BLOOD PRESSURE AND INFLAMMATORY MARKERS IN TYPE 2 DIABETIC PATIENTS , 2008 .

[6]  S. S. Bahadur,et al.  The berberis story: Berberis vulgaris in therapeutics. , 2007, Pakistan journal of pharmaceutical sciences.

[7]  A. Krishnaraju,et al.  Biological Screening of Medicinal Plants Collected from Eastern Ghats of India Using Artemia salina (Brine Shrimp Test) , 2006 .

[8]  Y. Hsieh,et al.  Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. , 2006, Toxicology and applied pharmacology.

[9]  R. Kunwar,et al.  Ethnomedicine in Himalaya: a case study from Dolpa, Humla, Jumla and Mustang districts of Nepal , 2006, Journal of ethnobiology and ethnomedicine.

[10]  Hyun-Joung Lim,et al.  Berberine inhibits rat vascular smooth muscle cell proliferation and migration in vitro and improves neointima formation after balloon injury in vivo. Berberine improves neointima formation in a rat model. , 2006, Atherosclerosis.

[11]  G. Motalleb,et al.  EVALUATION OF PHENOLIC CONTENT AND TOTAL ANTIOXIDANT ACTIVITY IN BERBERIS VULGARIS FRUIT EXTRACT , 2005 .

[12]  C. Chi,et al.  The anti-inflammatory potential of berberine in vitro and in vivo. , 2004, Cancer letters.

[13]  R. Enriz,et al.  Antimicrobial activity of aqueous extracts and of berberine isolated from Berberis heterophylla. , 2003, Fitoterapia.

[14]  K. Kaur,et al.  Indian medicinal plants as a reservoir of protective phytochemicals. , 2003, Teratogenesis, carcinogenesis, and mutagenesis (Print).

[15]  B. Yung,et al.  Berberine complexes with DNA in the berberine-induced apoptosis in human leukemic HL-60 cells. , 1995, Cancer letters.

[16]  Yong-Xiao Wang,et al.  Effects of Berberine on Delayed Afterdepolarizations in Ventricular Muscles In Vitro and In Vivo , 1994, Journal of cardiovascular pharmacology.

[17]  K. Abe,et al.  [Pharmacological studies on antidiarrheal effects of a preparation containing berberine and geranii herba]. , 1993, Nihon yakurigaku zasshi. Folia pharmacologica Japonica.

[18]  S. N. Hooper,et al.  Herbal remedies of the Maritime Indians. , 1979, Journal of ethnopharmacology.