Relationship between the amounts of EBV‐DNA and ebna per cell, clonability and tumorigenicity in two EBV‐negative lymphoma lines and their EBV‐converted sublines

The effect of EBV‐conversion of two EBV‐negative lymphoma lines (Ramos and BJAB) on agarose clonability and tumorigenicity in nude mice was explored. The cloning frequency was increased in all 9 sublines investigated, between I.I and 4.9 times compared to the original “parental” lines. Tumorigenicity was increased in one out of 2 BJAB‐derived, EBV‐positive lines and in 3 of 6 Ramosderived lines, while it was decreased in 3 others. A strong positive correlation between the number of genomes/cell and the amount of EBNA/cell was detected, and in 7 of 9 converted lines the cloning frequency also correlated to the number of EBV genomes. On the other hand, no relation was established between the number of EBV‐genomes/cell, the amount of EBNA/cell and tumorigenicity.

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