Enzymes from human gastric mucosa conferring blood-group A and B specificities upon erythrocytes.

Enzyme preparations obtained from human gastric mucosa microsomes are able to provide human erythrocytes with blood-group A and B specificities, the newly conferred serological properties being dependent on the blood-group of the tissue donor. The time-course and the extent of the enzyme-catalyzed change in antigenic properties of the erythrocytes were assessed by the serum absorption method. The enzyme preparation from gastric mucosa of group A1 individuals, when incubated with O or B erythrocytes in the presence of UDP-acetylgalactosamine, rendered them agglutin-able by anti-A serum. The enzymatically converted erythrocytes behaved like A2 cells. After treatment with enzyme from B individuals and UDP-galactose, O, A1 and A2 erythrocytes could be agglutinated by anti-B serum. When the enzymes from A1 and B individuals were allowed to act together upon O erythrocytes in the presence of UDP-acetylgalactosamine and UDP-galactose, the red cells became susceptible to agglutination with both anti-A and anti-B serum. The blood-group specificity of red cells was not changed upon exposure to microsomal extracts from O individuals.

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