Hydrophobins: Proteins that self assemble at interfaces

Hydrophobins are surface active proteins that are produced by filamentous fungi. They are interesting from a Surf Sci point of view because some of their properties as surface active proteins are quite spectacular. In this review, recent advances in understanding these properties will be surveyed. We will attempt to define what the properties are that make them unique. As an understanding of both structure and function of hydrophobins is emerging we see that this is paving the way for industrial applications as well as an understanding of their biological functions. Major recent advances Recently there has been a clear increase in attempts to use hydrophobins in applications. We are starting to understand their unique properties as surfactants and especially applications related to the stability and development of foams and various surface treatments are emerging. There are several new reports on molecular structures as well on mechanisms of self-assembly. Hydrophobins have functions in biology that are far from understood, but also here techniques are developing and a broader understanding is emerging.

[1]  M. Penttilä,et al.  Direct identification of hydrophobins and their processing in Trichoderma using intact‐cell MALDI‐TOF MS , 2007, The FEBS journal.

[2]  M. Izzard,et al.  Surface properties of class ii hydrophobins from Trichoderma reesei and influence on bubble stability. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[3]  A. B. Russell,et al.  Designing Multiscale Structures for Desired Properties of Ice Cream , 2008 .

[4]  Markus B Linder,et al.  The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei , 2007, FEBS letters.

[5]  Joel P Mackay,et al.  Structural analysis of hydrophobins. , 2008, Micron.

[6]  N J Talbot,et al.  Hydrophobins and repellents: proteins with fundamental roles in fungal morphogenesis. , 1998, Fungal genetics and biology : FG & B.

[7]  H. Wösten,et al.  Fungal hydrophobins in medical and technical applications , 2001, Applied Microbiology and Biotechnology.

[8]  J. Mackay,et al.  Structural basis for rodlet assembly in fungal hydrophobins. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[9]  Merja Penttilä,et al.  Atomic Resolution Structure of the HFBII Hydrophobin, a Self-assembling Amphiphile* , 2004, Journal of Biological Chemistry.

[10]  A. B. Russell,et al.  Exceptional stability of food foams using class II hydrophobin HFBII. , 2009 .

[11]  B. Murray Stabilization of bubbles and foams , 2007 .

[12]  R. Serimaa,et al.  Interactions of hydrophobin proteins in solution studied by small-angle X-ray scattering. , 2008, Biophysical journal.

[13]  K. Scholtmeijer,et al.  Hydrophobins: proteins with potential. , 2005, Current opinion in biotechnology.

[14]  Marc Reisch INVIGORATING R&D: CORNING AVOIDED FINANCIAL DISASTER thanks in part to its 100-year-old R&D organization , 2008 .

[15]  Thomas Subkowski,et al.  Industrial performance proteins: Hydrophobin—learning from nature , 2007 .

[16]  Hao Fan,et al.  Molecular dynamics simulations of the hydrophobin SC3 at a hydrophobic/hydrophilic interface , 2006, Proteins.

[17]  Merja Penttilä,et al.  Structural hierarchy in molecular films of two class II hydrophobins. , 2003, Biochemistry.

[18]  M. Torkkeli,et al.  Self‐assembled structures of hydrophobins HFBI and HFBII , 2003 .

[19]  Olli Ikkala,et al.  Precisely defined protein-polymer conjugates: construction of synthetic DNA binding domains on proteins by using multivalent dendrons. , 2007, ACS nano.

[20]  J. Latgé,et al.  Combined use of atomic force microscopy, X-ray photoelectron spectroscopy, and secondary ion mass spectrometry for cell surface analysis. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[21]  P. Spanu,et al.  Localization of Cladosporium fulvum hydrophobins reveals a role for HCf-6 in adhesion. , 2008, FEMS microbiology letters.

[22]  E. Lacaze,et al.  Langmuir-Blodgett film of hydrophobin protein from Pleurotus ostreatus at the air-water interface. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[23]  H. Jiang,et al.  Controlled hybrid nanostructures through protein-mediated noncovalent functionalization of carbon nanotubes. , 2007, Angewandte Chemie.

[24]  J. Jänis,et al.  Protein HGFI from the edible mushroom Grifola frondosa is a novel 8 kDa class I hydrophobin that forms rodlets in compressed monolayers. , 2008, Microbiology.

[25]  Xia Qin,et al.  Amperometric glucose biosensor based on self-assembly hydrophobin with high efficiency of enzyme utilization. , 2007, Biosensors & bioelectronics.

[26]  Tiina Nakari-Setälä,et al.  Hydrophobins: the protein-amphiphiles of filamentous fungi. , 2005, FEMS microbiology reviews.

[27]  K. F. Dobinson,et al.  A hydrophobin gene, VDH1, is involved in microsclerotial development and spore viability in the plant pathogen Verticillium dahliae. , 2006, Fungal genetics and biology : FG & B.

[28]  H. Maeda,et al.  The fungal hydrophobin RolA recruits polyesterase and laterally moves on hydrophobic surfaces , 2005, Molecular microbiology.

[29]  G. Robillard,et al.  Probing the self-assembly and the accompanying structural changes of hydrophobin SC3 on a hydrophobic surface by mass spectrometry. , 2004, Biophysical journal.

[30]  M. Rillig A connection between fungal hydrophobins and soil water repellency , 2005 .

[31]  O. Ikkala,et al.  Multivalent dendrons for high-affinity adhesion of proteins to DNA. , 2006, Angewandte Chemie.

[32]  Helge Lemmetyinen,et al.  Self-assembled films of hydrophobin protein HFBIII from Trichoderma reesei , 2007 .

[33]  A. Beauvais,et al.  An extracellular matrix glues together the aerial‐grown hyphae of Aspergillus fumigatus , 2007, Cellular microbiology.

[34]  Helge Lemmetyinen,et al.  Langmuir-Blodgett films of hydrophobins HFBI and HFBII , 2005 .

[35]  I. Rendina,et al.  Self-assembled biofilm of hydrophobins protects the silicon surface in the KOH wet etch process. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[36]  S. Jeong,et al.  Isolation of genes expressed during the developmental stages of the oyster mushroom, Pleurotus ostreatus, using expressed sequence tags. , 2007, FEMS microbiology letters.

[37]  N. Keyhani,et al.  Adhesion of the Entomopathogenic Fungus Beauveria (Cordyceps) bassiana to Substrata , 2005, Applied and Environmental Microbiology.

[38]  Helge Lemmetyinen,et al.  Self-assembled hydrophobin protein films at the air-water interface: structural analysis and molecular engineering. , 2007, Biochemistry.

[39]  G. Robillard,et al.  The SC3 hydrophobin self-assembles into a membrane with distinct mass transfer properties. , 2005, Biophysical journal.

[40]  Juha Rouvinen,et al.  Two crystal structures of Trichoderma reesei hydrophobin HFBI—The structure of a protein amphiphile with and without detergent interaction , 2006, Protein science : a publication of the Protein Society.

[41]  A. Walcarius,et al.  Analytical investigation of the interactions between SC3 hydrophobin and lipid layers: elaborating of nanostructured matrixes for immobilizing redox systems. , 2006, Analytical Chemistry.

[42]  J. Wessels DEVELOPMENTAL REGULATION OF FUNGAL CELL-WALL FORMATION , 1994 .

[43]  G. Robillard,et al.  Oligomerization of hydrophobin SC3 in solution: From soluble state to self‐assembly , 2004, Protein science : a publication of the Protein Society.

[44]  Juha Rouvinen,et al.  Crystal Structures of Hydrophobin HFBII in the Presence of Detergent Implicate the Formation of Fibrils and Monolayer Films* , 2007, Journal of Biological Chemistry.

[45]  H. Wösten,et al.  Role of proteins in soil carbon and nitrogen storage: controls on persistence , 2007 .

[46]  G. Robillard,et al.  Self‐assembly of the hydrophobin SC3 proceeds via two structural intermediates , 2002, Protein science : a publication of the Protein Society.

[47]  Chen Wang,et al.  Bioactive surface modification of mica and poly(dimethylsiloxane) with hydrophobins for protein immobilization. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[48]  S. Herrera,et al.  Industrial biotechnology—a chance at redemption , 2004, Nature Biotechnology.

[49]  R. Vogel,et al.  Cloning of Wheat LTP1500 and Two Fusarium culmorum Hydrophobins in Saccharomyces cerevisiae and Assessment of Their Gushing Inducing Potential in Experimental Wort Fermentation , 2006 .

[50]  S. O. Lumsdon,et al.  Adsorption of hydrophobin proteins at hydrophobic and hydrophilic interfaces. , 2005, Colloids and surfaces. B, Biointerfaces.

[51]  D. Chandler Interfaces and the driving force of hydrophobic assembly , 2005, Nature.

[52]  A. Mark,et al.  The Cys3-Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity. , 2008, Journal of molecular biology.

[53]  Dennis Claessen,et al.  Amyloids — a functional coat for microorganisms , 2005, Nature Reviews Microbiology.

[54]  H. Wösten,et al.  Hydrophobins, the fungal coat unravelled. , 2000, Biochimica et biophysica acta.

[55]  G. Cannon,et al.  Nanoscale reduction in surface friction of polymer surfaces modified with Sc3 hydrophobin from Schizophyllum commune. , 2006, Biomacromolecules.

[56]  G. Szilvay,et al.  Behavior of Trichoderma reesei hydrophobins in solution: interactions, dynamics, and multimer formation. , 2006, Biochemistry.

[57]  N. Talbot,et al.  Four conserved intramolecular disulphide linkages are required for secretion and cell wall localization of a hydrophobin during fungal morphogenesis , 2005, Molecular microbiology.

[58]  N. Talbot,et al.  Building filaments in the air: aerial morphogenesis in bacteria and fungi. , 2004, Current opinion in microbiology.

[59]  Merja Penttilä,et al.  Surface adhesion of fusion proteins containing the hydrophobins HFBI and HFBII from Trichoderma reesei , 2002, Protein science : a publication of the Protein Society.

[60]  H. Brückner,et al.  Recent Advances and Future Prospects in Peptaibiotics, Hydrophobin, and Mycotoxin Research, and Their Importance for Chemotaxonomy of Trichoderma and Hypocrea , 2008, Chemistry & biodiversity.

[61]  M. Penttilä,et al.  Fungal Hydrophobins as Predictors of the Gushing Activity of Malt , 2005 .

[62]  Merja Penttilä,et al.  Interaction and comparison of a class I hydrophobin from Schizophyllum commune and class II hydrophobins from Trichoderma reesei. , 2006, Biomacromolecules.

[63]  Ivo Rendina,et al.  Protein‐Modified Porous Silicon Nanostructures , 2008 .

[64]  P. Spanu,et al.  Hydrophobins and the interactions between fungi and plants. , 2002, Molecular plant pathology.

[65]  Helge Lemmetyinen,et al.  Self-assembled films of hydrophobin proteins HFBI and HFBII studied in situ at the air/water interface. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[66]  Olli Ikkala,et al.  Aggregation and self-assembly of hydrophobins from Trichoderma reesei: low-resolution structural models. , 2002, Biophysical journal.

[67]  Andrea Schmidt,et al.  Hydrophobin HFBII in detail: ultrahigh-resolution structure at 0.75 A. , 2006, Acta crystallographica. Section D, Biological crystallography.

[68]  G. Robillard,et al.  Structural and Functional Role of the Disulfide Bridges in the Hydrophobin SC3* , 2000, The Journal of Biological Chemistry.

[69]  M. Penttilä,et al.  Process technological effects of deletion and amplification of hydrophobins I and II in transformants of Trichoderma reesei , 2002, Applied Microbiology and Biotechnology.

[70]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.