Analysis of Epitopes of Mouse Monoclonal Antibodies against Human Alpha-Fetoprotein

Thirty-six monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFP) were analyzed for the location of their epitopes by reacting them with a set of yeast recombinant AFP proteins using ELISA. Recombinant AFP proteins containing either one, two or all three domains, i.e. domain I, domain III, domain I-II, domain II-III and domain I-II-III, were produced and secreted into the culture medium of yeast cells harboring the expression plasmids. Epitopes of 13 MAbs were localized on domain I and 17 others were on domain III. However, the exact location of the epitopes of the remaining 6 MAbs could not be defined. The epitope of an antibody, namely AFY6, which was located in domain I, was successfully mapped on an octapeptide, C175KAENAVE182, using synthesized overlapping octapeptides.

[1]  G. Abelev,et al.  Epitope Mapping of Human Alpha-Fetoprotein , 1998, Tumor Biology.

[2]  E. Alpert,et al.  Summary Report: Epitope Analysis of Human Alpha-Fetoprotein , 1998, Tumor Biology.

[3]  K. Taketa,et al.  Lectin-Dependent Modulation of Interaction between Human Alpha-Fetoprotein and Its Monoclonal Antibodies , 1998, Tumor Biology.

[4]  K. Nustad,et al.  Specificity and Affinity of 30 Monoclonal Antibodies against Alpha-Fetoprotein , 1998, Tumor Biology.

[5]  K. Taketa,et al.  Biochemical Characterization of α‐Fetoprotein and Other Serum Proteins Produced by a Uterine Endometrial Adenocarcinoma , 1996, Japanese journal of cancer research : Gann.

[6]  S. Nishi,et al.  Expression of rat alpha-fetoprotein cDNA and its mutants in cultured mouse fibroblasts and identification of glycosylation sites related to electrophoretic variants. , 1994, Biochimica et biophysica acta.

[7]  H. Matsue,et al.  Localization of the estrogen-binding site of alpha-fetoprotein in the chimeric human-rat proteins. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[8]  H. F. Deutsch.  Chemistry and biology of α-fetoprotein , 1991 .

[9]  D. Goldenberg,et al.  Carcinoembryonic Antigen and α-Fetoprotein Expression and Monoclonal Antibody Targeting in a Human Hepatoma/Nude Mouse Model , 1990 .

[10]  M. Engelhard,et al.  Epitope mapping employing antibodies raised against short synthetic peptides: a study of the nicotinic acetylcholine receptor. , 1989, Biochemistry.

[11]  G. Abelev.  Alpha-fetoprotein: 25 years of study. , 1989 .

[12]  H. M. Geysen,et al.  Strategies for epitope analysis using peptide synthesis. , 1987, Journal of immunological methods.

[13]  M. Tokunaga,et al.  A novel yeast secretion vector utilizing secretion signal of killer toxin encoded on the yeast linear DNA plasmid pGKL1. , 1987, Biochemical and biophysical research communications.

[14]  S. Nishi,et al.  MONOCLONAL ANTIBODIES AGAINST AFP: APPLICATION IN IMMUNOASSAY AND AFFINITY CHROMATOGRAPHY , 1983, Annals of the New York Academy of Sciences.

[15]  T. Tamaoki,et al.  Primary structures of human alpha-fetoprotein and its mRNA. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[16]  N. Ishii,et al.  Localization of Radioiodinated Antibody to α-Fetoprotein in Hepatoma Transplanted in Rats and a Case Report of α-Fetoprotein Antibody Treatment of a Hepatoma Patient , 1980 .

[17]  H. Hirai,et al.  Purification of human, dog and rabbit -fetoprotein by immunoadsorbents of sepharose coupled with anti-human -fetoprotein. , 1972, Biochimica et biophysica acta.