Translating In Vitro Cell Lines Result into Clinical Practice

Immortalized cell lines provide an easy and convenient option for analysis of biological systems compared to clinical specimens. Cell culture has gained wide popularity in the study of cancer, because of the innate continuously proliferating nature of these cell lines. Cancer cell lines are obtained by the enzymatic digestion or explant growth of tumour specimens. The main advantage of using cell lines for such research is the immortal nature of the cell lines, enabling them to be continuously cultured, distributed and studied in many labs and to act as a reliable platform for comparison of results, before advancing research to the next level. Another advantage of performing research on cell lines is ease of handling and storage. Cell lines are cultured in flasks under well-controlled nutritional and environmentalal conditions and this ensures a greater degree of reproducibility in the results. Some assays require large amount of material; with cell line models, this is generally not a constraint. Cells can be stored indefinitely in liquid nitrogen and can be used when needed. Apart from the ease in maintenance and access, cell lines offer a convenient platform for genetic manipulation of cells. Many recent studies in cancer have centered on functional aspects of gene expression changes. Cell lines offer a realistic platform to knock-down or over-express genes of interest. Due to the tremendous benefits of working on cell lines, they have dramatically contributed to basic research in cancer biology.

[1]  R. Sandberg,et al.  The molecular portrait of in vitro growth by meta-analysis of gene-expression profiles , 2005, Genome Biology.

[2]  D. Botstein,et al.  Cluster analysis and display of genome-wide expression patterns. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[3]  D. G. Newell,et al.  Cell & Tissue Culture: Laboratory Procedures , 1993 .

[4]  M. Westerfield,et al.  Characterization of paired tumor and non‐tumor cell lines established from patients with breast cancer , 1998, International journal of cancer.

[5]  Adam Ertel,et al.  Pathway-specific differences between tumor cell lines and normal and tumor tissue cells , 2006, Molecular Cancer.

[6]  T. Chen,et al.  Mycoplasma infection of cultured cells , 1989, Nature.

[7]  A microarray approach to translational medicine in breast cancer: how representative are cell line models of clinical conditions? , 2007, Anticancer research.

[8]  Joshua M. Stuart,et al.  MICROARRAY EXPERIMENTS : APPLICATION TO SPORULATION TIME SERIES , 1999 .

[9]  M. Lacroix,et al.  Relevance of Breast Cancer Cell Lines as Models for Breast Tumours: An Update , 2004, Breast Cancer Research and Treatment.

[10]  I. Tannock,et al.  Drug resistance and the solid tumor microenvironment. , 2007, Journal of the National Cancer Institute.

[11]  H. Drexler,et al.  Widespread intraspecies cross‐contamination of human tumor cell lines arising at source , 1999, International journal of cancer.

[12]  J A Thomson,et al.  Short tandem repeat profiling provides an international reference standard for human cell lines , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[13]  John R. W. Masters,et al.  Human cancer cell lines: fact and fantasy , 2000, Nature Reviews Molecular Cell Biology.

[14]  M. Lacroix,et al.  Persistent use of “false” cell lines , 2008, International journal of cancer.

[15]  W. Nelson-Rees,et al.  Cross-contamination of cells in culture. , 1981, Science.

[16]  Valerie Speirs,et al.  Breast cancer cell lines: friend or foe? , 2003, Breast Cancer Research.

[17]  M. Gottesman,et al.  Comparing solid tumors with cell lines: implications for identifying drug resistance genes in cancer. , 2004, Molecular interventions.

[18]  Stefanie S Jeffrey,et al.  A molecular 'signature' of primary breast cancer cultures; patterns resembling tumor tissue , 2004, BMC Genomics.

[19]  T. Litman,et al.  A Serial Analysis of Gene Expression (SAGE) database analysis of chemosensitivity: comparing solid tumors with cell lines and comparing solid tumors from different tissue origins. , 2004, Cancer research.

[20]  Charles M. Perou,et al.  A Comparison of Gene Expression Signatures from Breast Tumors and Breast Tissue Derived Cell Lines , 2002, Disease markers.

[21]  R. Tibshirani,et al.  Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[22]  C. Osborne,et al.  Biological differences among MCF-7 human breast cancer cell lines from different laboratories , 2005, Breast Cancer Research and Treatment.

[23]  P. Drew,et al.  Karyotypic variation between independently cultured strains of the cell line MCF-7 identified by multicolour fluorescence in situ hybridization. , 2002, International journal of oncology.