Hematopoietic mutations in the zebrafish.

We have identified mutations that perturb the formation or differentiation of the first embryonic blood cells in the zebrafish embryo. These 'primitive' red blood cells originate in the intermediate cell mass of the trunk, a derivative of the dorsal lateral plate mesoderm. By transfusion of blood between embryos we demonstrate that this cohort of cells provides the embryo with all, or nearly all, of its blood cells until at least day 5 postfertilization. Larval lethal mutations generated by ENU mutagenesis affect different steps in the development of these cells. Some cause defects in precursor generation, others defects in differentiation, and others an increase in cellular photosensitivity.

[1]  A. Schier,et al.  A genetic screen for mutations affecting embryogenesis in zebrafish. , 1996, Development.

[2]  D. Ransom,et al.  Intraembryonic hematopoietic cell migration during vertebrate development. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[3]  L. Zon,et al.  Cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. , 1995, Development.

[4]  Janet Rossant,et al.  Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice , 1995, Nature.

[5]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[6]  C. Wernstedt,et al.  Identification of the Major Phosphorylation Sites for Protein Kinase C in Kit/Stem Cell Factor Receptor in Vitro and in Intact Cells (*) , 1995, The Journal of Biological Chemistry.

[7]  S. Orkin,et al.  Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCL , 1995, Nature.

[8]  Stuart H. Orkin,et al.  An early haematopoietic defect in mice lacking the transcription factor GATA-2 , 1994, Nature.

[9]  M. Evans,et al.  The Oncogenic Cysteine-rich LIM domain protein Rbtn2 is essential for erythroid development , 1994, Cell.

[10]  G. Keller,et al.  Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells. , 1994, Genes & development.

[11]  J. García-Porrero,et al.  Para-aortic splanchnopleura from early mouse embryos contains B1a cell progenitors , 1993, Nature.

[12]  A. Müller,et al.  An early pre-liver intraembryonic source of CFU-S in the developing mouse , 1993, Nature.

[13]  Y. Ikawa,et al.  Primitive erythropoiesis of mouse teratocarcinoma stem cells PCC3/A/1 in serum-free medium. , 1991, Development.

[14]  S. Orkin,et al.  Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1 , 1991, Nature.

[15]  W. Cohen,et al.  The cytoskeletal system of mammalian primitive erythrocytes: studies in developing marsupials. , 1990, Cell motility and the cytoskeleton.

[16]  R. Bernstein,et al.  Fanconi's anemia--chromosome breakage studies in homozygotes and heterozygotes. , 1988, Cancer genetics and cytogenetics.

[17]  A. Auerbach.  A test for Fanconi's anemia. , 1988, Blood.

[18]  F. Dieterlen-Lièvre,et al.  Diffuse intraembryonic hemopoiesis in normal and chimeric avian development. , 1981, Developmental biology.

[19]  C. Knudson,et al.  The early ontogeny of hematopoietic cells studied by grafting cytogenetically labeled tissue anlagen: localization of a prospective stem cell compartment. , 1981, Developmental biology.

[20]  M. Freedman,et al.  Constitutional Aplastic Anaemia: Defective Haematopoietic Stem Cell Growth in Vitro , 1978, British journal of haematology.

[21]  Y. Kunz,et al.  ONTOGENESIS OF HAEMATOPOIETIC SITES IN BRACHYDANIO RERIO (HAMILTON‐BUCHANAN) (TELEOSTEI) * , 1977, Development, growth & differentiation.

[22]  N. Goya,et al.  A family of congenital atransferrinemia. , 1972, Blood.

[23]  L. Diamond,et al.  Chromosome abnormalities in constitutional aplastic anemia. , 1966, The New England journal of medicine.