Development and Validation of a Simultaneous Analytical Method for the Detection of Mefentrifluconazole and Triticonazole Fungicide in Agricultural Crops

BACKGROUND: Mefentrifluconazole and triticonazole are the triazole fungicides. The maximum residue levels for agricultural products need to be set up. Therefore, development of the official analytical method for determination of mefentrifluconazole and triticonazole residues from agricultural crops was necessary due to safety management, and then a simultaneous analytical method was developed for the determination of mefentrifluconazole and triticonazole in agricultural crops. METHODS AND RESULTS: Samples were extracted using acetonitrile and purified using dispersive solid phase extraction, and then detected with liquid chromatographtandem mass spectrometry (LC-MS/MS). Matrix-matched calibration curves (0.0025-0.25 μg/mL) were linear into a sample extract with r>0.99. For validation, the recovery test was carried out at three fortification levels (LOQ, 10 LOQ and 50 LOQ) from agricultural samples. The results for mefentrifluconazole and triticonazole ranged between 92.3 to 115.3% and 91.4 to 108.5%, respectively and RSD (relative standard deviation) values were also below 6.0%. Furthermore, inter-laboratory was conducted to validate the method. CONCLUSION: All values were corresponded with the criteria ranges requested by both the CODEX (CAC/GL *Corresponding author: Sang Soon Yun Phone: +82-43-719-4211; Fax: +82-43-719-4200; E-mail: yss0520@korea.kr Korean Journal of Environmental Agriculture Korean J Environ Agric. 2020;39(2):130-137. Korean Online ISSN: 2233-4173 Published online 2020 June 26. https://doi.org/10.5338/KJEA.2020.39.2.17 Print ISSN: 1225-3537 농산물 중 메펜트리플루코나졸 및 트리티코나졸 살균제의