Association between homozygous Methylene Tetrahydrofolate Reductase and Platelet PLA2 Antigen Polymorphisms with Venous Thromboembolism (VTE) in Shahrekord

Background and Objective: Venous thromboembolism (VTE) is one of the main causes of mortality in different human communities. Factor V Leiden, MTHFR C 677 T polymorphism and PLA 2 polymorphism of platelet glycoprotein IIb/IIIa are important inheritance risk factors of VTE. As limited data of homozygous factors exists, the aim of the study was to investigate the association between homozygous polymorphisms and VTE in Shahrekord. Materials and Methods: In this cross- sectional study, EDTA venous blood was taken from 72 patients with venous thromboembolism referred to Shahrekord Hajar Hospital, and 306 age and sex matched healthy volunteers. Genotyping of factor V Leiden, MTHFR C 677 T and PLA 2 polymorphisms was done by PCR-RFLP. Statistical analysis was done using descriptive statistics and χ 2 test. Results: There was a significant difference between patients and controls in the number of homozygous polymorphisms with the frequency of 16.77% and 4.90%, respectively (P=0.004). Homozygous FVL was more prevalent in patients than controls, but the difference was not significant. A significant difference was observed in the frequency of homozygous MTHFR C 677 T and PLA 2 polymorphisms between the patients and controls (P= 0.03 and P=0.001, respectively). The difference was not significant in the frequency of heterozygous polymorphisms except for PLA 2 . Conclusion: The results of the study show the association between homozygous MTHFR and PLA 2 thrombophilia polymorphisms and VTE in Shahrekord. Acquired risk factors may need to be controlled in homozygous carriers of these polymorphisms. Homozygous VTE carriers may need to be managed differently than other patients.