Community Structure Analyses of Anodic Biofilms in a Bioelectrochemical System Combined with an Aerobic Reactor

Bioelectrochemical system (BES)-based reactors have a limited range of use, especially in aerobic conditions, because these systems usually produce current from exoelectrogenic bacteria that are strictly anaerobic. However, some mixed cultures of bacteria in aerobic reactors can form surface biofilms that may produce anaerobic conditions suitable for exoelectrogenic bacteria to thrive. In this study, we combined a BES with an aerobic trickling filter (TF) reactor for wastewater treatment and found that the BES-TF setup could produce electricity with a coulombic efficiency of up to 15% from artificial wastewater, even under aerobic conditions. The microbial communities within biofilms formed at the anodes of BES-TF reactors were investigated using high throughput 16S rRNA gene sequencing. Efficiency of reduction in chemical oxygen demand and total nitrogen content of wastewater using this system was >97%. Bacterial community analysis showed that exoelectrogenic bacteria belonging to the genera Geobacter and Desulfuromonas were dominant within the biofilm coating the anode, whereas aerobic bacteria from the family Rhodocyclaceae were abundant on the surface of the biofilm. Based on our observations, we suggest that BES-TF reactors with biofilms containing aerobic bacteria and anaerobic exoelectrogenic bacteria on the anodes can function in aerobic environments.

[1]  Heming Wang,et al.  Bioelectrochemical system platform for sustainable environmental remediation and energy generation. , 2015, Biotechnology advances.

[2]  Steven Salzberg,et al.  BIOINFORMATICS ORIGINAL PAPER , 2004 .

[3]  Jiuhui Qu,et al.  Application of Integrated Bioelectrochemical-Wetland Systems for Future Sustainable Wastewater Treatment. , 2019, Environmental science & technology.

[4]  H. Biebl,et al.  Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium , 1976, Archives of Microbiology.

[5]  Anne S. Meyer,et al.  Acetate is a superior substrate for microbial fuel cell initiation preceding bioethanol effluent utilization , 2015, Applied Microbiology and Biotechnology.

[6]  Heming Wang,et al.  A comprehensive review of microbial electrochemical systems as a platform technology. , 2013, Biotechnology advances.

[7]  Kai Finster,et al.  Desulfuromonas acetexigens sp. nov., a dissimilatory sulfur-reducing eubacterium from anoxic freshwater sediments , 1994, Archives of Microbiology.

[8]  D. R. Bond,et al.  Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments , 2002, Science.

[9]  K. Haga,et al.  Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry , 2007, Applied Microbiology and Biotechnology.

[10]  E. E. L O G A N,et al.  Production of Electricity during Wastewater Treatment Using a Single Chamber Microbial Fuel Cell , 2022 .

[11]  Björn Usadel,et al.  Trimmomatic: a flexible trimmer for Illumina sequence data , 2014, Bioinform..

[12]  Rob Knight,et al.  PyNAST: a flexible tool for aligning sequences to a template alignment , 2009, Bioinform..

[13]  B. Logan.  Exoelectrogenic bacteria that power microbial fuel cells , 2009, Nature Reviews Microbiology.

[14]  H. Kanamori,et al.  Enhanced electrical power generation using flame-oxidized stainless steel anode in microbial fuel cells and the anodic community structure , 2016, Biotechnology for Biofuels.

[15]  E. Roden,et al.  Dissimilatory Fe(III) Reduction by the Marine Microorganism Desulfuromonas acetoxidans , 1993, Applied and environmental microbiology.

[16]  J. G. Kuenen,et al.  Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor , 1995 .

[17]  T. Yamashita,et al.  Molybdenum anode: a novel electrode for enhanced power generation in microbial fuel cells, identified via extensive screening of metal electrodes , 2018, Biotechnology for Biofuels.

[18]  Lu Lu,et al.  Microbial fuel cells and osmotic membrane bioreactors have mutual benefits for wastewater treatment and energy production. , 2016, Water research.

[19]  Catherine Larose,et al.  Combined iron and sulfate reduction biostimulation as a novel approach to enhance BTEX and PAH source-zone biodegradation in biodiesel blend-contaminated groundwater. , 2017, Journal of hazardous materials.

[20]  B. Logan,et al.  Assessment of Microbial Fuel Cell Configurations and Power Densities , 2015 .

[21]  Torsten Wik,et al.  Trickling Filters and Biofilm Reactor Modelling , 2003 .

[22]  Zhichao Wu,et al.  Power production from different types of sewage sludge using microbial fuel cells: A comparative study with energetic and microbiological perspectives , 2013 .

[23]  S. Giovannoni,et al.  Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals , 2004, Archives of Microbiology.

[24]  In S. Kim,et al.  Effect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells. , 2009, Bioresource technology.

[25]  Y. Kitamura,et al.  Community analysis of biofilms on flame-oxidized stainless steel anodes in microbial fuel cells fed with different substrates , 2017, BMC Microbiology.

[26]  Flynn W. Picardal,et al.  Desulfuromonas carbonis sp. nov., an Fe(III)-, S0- and Mn(IV)-reducing bacterium isolated from an active coalbed methane gas well. , 2015, International journal of systematic and evolutionary microbiology.

[27]  K. Baalsrud,et al.  Studies on thiobacillus denitrificans , 2004, Archiv für Mikrobiologie.

[28]  Willy Verstraete,et al.  Minimizing losses in bio-electrochemical systems: the road to applications , 2008, Applied Microbiology and Biotechnology.

[29]  Aharon Oren,et al.  The Family Rhodocyclaceae , 2014 .

[30]  Eoin L. Brodie,et al.  Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB , 2006, Applied and Environmental Microbiology.

[31]  J. Ni,et al.  Palm oil mill effluent treatment using a two-stage microbial fuel cells system integrated with immobilized biological aerated filters. , 2010, Bioresource technology.

[32]  Takahiro Yamashita,et al.  A novel open-type biosensor for the in-situ monitoring of biochemical oxygen demand in an aerobic environment , 2016, Scientific Reports.

[33]  Bruce E Logan,et al.  Hydrogen production in a single chamber microbial electrolysis cell lacking a membrane. , 2008, Environmental science & technology.

[34]  William A. Walters,et al.  Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms , 2012, The ISME Journal.

[35]  Bruce E. Logan,et al.  A Two-Stage Microbial Fuel Cell and Anaerobic Fluidized Bed Membrane Bioreactor (MFC-AFMBR) System for Effective Domestic Wastewater Treatment , 2014, Environmental science & technology.

[36]  Fenglin Yang,et al.  Development of a novel proton exchange membrane-free integrated MFC system with electric membrane bioreactor and air contact oxidation bed for efficient and energy-saving wastewater treatment. , 2017, Bioresource technology.

[37]  Yujie Feng,et al.  A combined system of microbial fuel cell and intermittently aerated biological filter for energy self-sufficient wastewater treatment , 2015, Scientific Reports.

[38]  Stefano Freguia,et al.  Microbial fuel cells: methodology and technology. , 2006, Environmental science & technology.

[39]  Mitsuyoshi Ishida,et al.  Treatment of cow-waste slurry by a microbial fuel cell and the properties of the treated slurry as a liquid manure , 2006 .

[40]  D. Nicholas,et al.  Sulphide oxidation linked to the reduction of nitrate and nitrite in Thiobacillus denitrificans. , 1973, Biochimica et biophysica acta.

[41]  Marcel Martin.  Cutadapt removes adapter sequences from high-throughput sequencing reads , 2011 .

[42]  Duu-Jong Lee,et al.  Efficient electricity generation from sewage sludge using biocathode microbial fuel cell. , 2012, Water research.

[43]  P. Legendre,et al.  vegan : Community Ecology Package. R package version 1.8-5 , 2007 .

[44]  Ralf Cord-Ruwisch,et al.  A Periplasmic and Extracellular c-Type Cytochrome ofGeobacter sulfurreducens Acts as a Ferric Iron Reductase and as an Electron Carrier to Other Acceptors or to Partner Bacteria , 1998, Journal of bacteriology.

[45]  Carreño Carreño,et al.  Evaluación de la diversidad taxonómica y funcional de la comunidad microbiana relacionada con el ciclo del nitrógeno en suelos de cultivo de arroz con diferentes manejos del tamo , 2020 .

[46]  T. Mehta,et al.  Extracellular electron transfer via microbial nanowires , 2005, Nature.

[47]  Zhen He,et al.  A novel method to characterize bacterial communities affected by carbon source and electricity generation in microbial fuel cells using stable isotope probing and Illumina sequencing. , 2015, Journal of microbiological methods.

[48]  Robert C. Edgar,et al.  UPARSE: highly accurate OTU sequences from microbial amplicon reads , 2013, Nature Methods.

[49]  Fred A. Wright,et al.  Complete Genome Sequence of a Novel Bacterium within the Family Rhodocyclaceae That Degrades Polycyclic Aromatic Hydrocarbons , 2015, Genome Announcements.

[50]  Hubertus V M Hamelers,et al.  Bioelectrochemical systems: an outlook for practical applications. , 2012, ChemSusChem.