Activity Sources from Fast Large‐scale Brain Recordings in Adult Drosophila

We provide a method to record near‐whole brain activity in behaving adult flies and extract signals from specific anatomical structures. We image pan‐neuronal calcium or voltage sensors fluorescence at high speed with light field microscopy. We then apply computational methods to extract functional maps, and find that their characteristic shape match the anatomy of sub‐ neuropile regions and sometimes small population of neurons. Associated time series are also consistent with the literature.

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