Local forest environment largely affects below‐ground growth, clonal diversity and fine‐scale spatial genetic structure in the temperate deciduous forest herb Paris quadrifolia

Paris quadrifolia (herb Paris) is a long‐lived, clonal woodland herb that shows strong differences in local population size and shoot density along an environmental gradient of soil and light conditions. This environmentally based structuring may be mediated by differences in clonal growth and seedling recruitment through sexual reproduction. To study the interrelationship between environmental conditions and spatial patterns of clonal growth, the spatial genetic structure of four P. quadrifolia populations growing in strongly contrasting sites was determined. In the first place, plant excavations were performed in order to (i) determine differences in below‐ground growth of genets, (ii) investigate connectedness of ramets and (iii) determine total genet size. Although no differences in internode length were found among sites, clones in moist sites were much smaller (genets usually consisted of 1–3 interconnected shoots, most of them flowering) than genets in dry sites, which consisted of up to 15 interconnected shoots, the majority of which were vegetative. Further, amplified fragment length polymorphism (AFLP) markers were used. Clonal diversity was higher in populations located in moist and productive ash–poplar forests compared to those found in drier and less productive mixed forest sites (G/N: 0.27 and 0.14 and Simpson's D: 0.84 and 0.75, respectively). Patterns of spatial population genetic structure under dry conditions revealed several large clones dominating the entire population, whereas in moist sites many small genets were observed. Nevertheless, strong spatial genetic structure of the genet population was observed. Our results clearly demonstrate that patterns of clonal diversity and growth form of P. quadrifolia differ among environments. Limited seedling recruitment and large clone sizes due to higher connectedness of ramets explain the low clonal diversity in dry sites. In moist sites, higher levels of clonal diversity and small clone sizes indicate repeated seedling recruitment, whereas strong spatial genetic structure suggests limited seed dispersal within populations.

[1]  R. Brys,et al.  Sexual reproduction, clonal diversity and genetic differentiation in patchily distributed populations of the temperate forest herb Paris quadrifolia (Trilliaceae) , 2006, Oecologia.

[2]  C. Eckert,et al.  Trade‐offs between sexual and clonal reproduction in an aquatic plant: experimental manipulations vs. phenotypic correlations , 2004, Journal of evolutionary biology.

[3]  O. Hardy,et al.  New insights from fine‐scale spatial genetic structure analyses in plant populations , 2004, Molecular ecology.

[4]  O. Hardy,et al.  Estimation of pairwise relatedness between individuals and characterization of isolation‐by‐distance processes using dominant genetic markers , 2003, Molecular ecology.

[5]  O. Hardy,et al.  spagedi: a versatile computer program to analyse spatial genetic structure at the individual or population levels , 2002 .

[6]  R. Peakall,et al.  Breeding system, genetic diversity and clonal structure in the sub-alpine forb Rutidosis leiolepis F. Muell. (Asteraceae) , 2002 .

[7]  H. Jacquemyn,et al.  Possible effects of habitat fragmentation and climate change on the range of forest plant species , 2002 .

[8]  Charles B. Halpern,et al.  Clonal development of Maianthemum dilatatum in forests of differing age and structure , 2001 .

[9]  M. Fischer,et al.  Effects of intraspecific competition on size variation and reproductive allocation in a clonal plant , 2001 .

[10]  I. Jónsdóttir,et al.  Genetic variation and clonal diversity in four clonal sedges (Carex) along the Arctic coast of Eurasia , 2001, Molecular ecology.

[11]  B. Schmid,et al.  Genetic differentiation of life‐history traits within populations of the clonal plant Ranunculus reptans , 2000 .

[12]  Johan Ehrlén,et al.  DISPERSAL LIMITATION AND PATCH OCCUPANCY IN FOREST HERBS , 2000 .

[13]  R. Holderegger,et al.  Spatial genetic structure and clonal diversity of Anemone nemorosa in late successional deciduous woodlands of Central Europe , 2000 .

[14]  A. Depicker,et al.  AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.) , 2000, Molecular Breeding.

[15]  C. Eckert.  CONTRIBUTIONS OF AUTOGAMY AND GEITONOGAMY TO SELF-FERTILIZATION IN A MASS-FLOWERING, CLONAL PLANT , 2000 .

[16]  S. Kalisz,et al.  ANT-MEDIATED SEED DISPERSAL ALTERS PATTERN OF RELATEDNESS IN A POPULATION OF TRILLIUM GRANDIFLORUM , 1999 .

[17]  C. Eckert.  Clonal plant research: proliferation, integration, but not much evolution , 1999 .

[18]  Marc Mangel,et al.  MODELING INVESTMENTS IN SEEDS, CLONAL OFFSPRING, AND TRANSLOCATION IN A CLONAL PLANT , 1999 .

[19]  H. Kudoh,et al.  Genet structure and determinants of clonal structure in a temperate deciduous woodland herb, Uvularia perfoliata , 1999 .

[20]  Y. Piquot,et al.  Variation in sexual and asexual reproduction among young and old populations of the perennial macrop , 1998 .

[21]  B. Schaal,et al.  Molecular Variation in Isolated Plant Populations , 1996 .

[22]  A. Watkinson,et al.  Seedling recruitment and the maintenance of clonal diversity in plant populations-a computer simulation of Ranunculus repens , 1993 .

[23]  C. Eckert,et al.  Clonal reproduction and patterns of genotypic diversity in Decodon verticillatus (Lythraceae) , 1993 .

[24]  D. G. Lloyd,et al.  Self- and Cross-Fertilization in Plants. II. The Selection of Self- Fertilization , 1992, International Journal of Plant Sciences.

[25]  N. Aspinwall,et al.  CLONAL STRUCTURE, GENOTYPIC DIVERSITY, AND SEED PRODUCTION IN POPULATIONS OF FILIPENDULA RUBRA (ROSACEAE) FROM THE NORTHCENTRAL UNITED STATES , 1992 .

[26]  R. Turkington.  THE GROWTH, DISTRIBUTION AND NEIGHBOUR RELATIONSHIPS OF TRIFOLIUM REPENS IN A PERMANENT PASTURE. VI. CONDITIONING EFFECTS BY NEIGHBOURS , 1989 .

[27]  John L. Harper,et al.  THE GROWTH, DISTRIBUTION AND NEIGHBOUR RELATIONSHIPS OF TRIFOLIUM REPENS IN A PERMANENT PASTURE , 1989 .

[28]  E. Pollak,et al.  On the theory of partially inbreeding finite populations. I. Partial selfing. , 1988, Genetics.

[29]  C. Loehle.  Partitioning of reproductive effort in clonal plants: a benefit-cost , 1987 .

[30]  S. Handel.  The Intrusion of Clonal Growth Patterns on Plant Breeding Systems , 1985, The American Naturalist.

[31]  Robert R. Sokal,et al.  The Principles and Practice of Statistics in Biological Research. , 1982 .

[32]  T. Givnish.  Outcrossing Versus Ecological Constraints in the Evolution of Dioecy , 1982, The American Naturalist.

[33]  A. Watkinson,et al.  CLONAL VARIATION IN POPULATIONS OF RANUNCULUS REPENS , 1979 .

[34]  G. Hegi,et al.  Illustrierte Flora von Mittel-Europa , 1975 .

[35]  E. C. Pielou,et al.  An introduction to mathematical ecology , 1970 .

[36]  F. Lefort,et al.  An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus , 1999 .

[37]  P. Vos,et al.  AFLP: a new technique for DNA fingerprinting. , 1995, Nucleic acids research.

[38]  B. Weir,et al.  Allozyme diversity in plant species. , 1990 .

[39]  N. Ellstrand,et al.  Patterns of Genotypic Diversity in Clonal Plant Species , 1987 .

[40]  R. Cook.  Growth and development in clonal plant populations , 1985 .

[41]  W. Abrahamson.  Demography and vegetative reproduction. , 1980 .

[42]  F. James Rohlf,et al.  Biometry: The Principles and Practice of Statistics in Biological Research , 1969 .

[43]  D. T.,et al.  The Reproductive Capacity of Plants , 1943, Nature.