Optical properties of the mouse eye

The Shack-Hartmann wavefront sensor (SHWS) spots upon which ocular aberration measurements depend have poor quality in mice due to light reflected from multiple retinal layers. We have designed and implemented a SHWS that can favor light from a specific retinal layer and measured monochromatic aberrations in 20 eyes from 10 anesthetized C57BL/6J mice. Using this instrument, we show that mice are myopic, not hyperopic as is frequently reported. We have also measured longitudinal chromatic aberration (LCA) of the mouse eye and found that it follows predictions of the water-filled schematic mouse eye. Results indicate that the optical quality of the mouse eye assessed by measurement of its aberrations is remarkably good, better for retinal imaging than the human eye. The dilated mouse eye has a much larger numerical aperture (NA) than that of the dilated human eye (0.5 NA vs. 0.2 NA), but it has a similar amount of root mean square (RMS) higher order aberrations compared to the dilated human eye. These measurements predict that adaptive optics based on this method of wavefront sensing will provide improvements in retinal image quality and potentially two times higher lateral resolution than that in the human eye.

[1]  Alfredo Dubra,et al.  Registration of 2D Images from Fast Scanning Ophthalmic Instruments , 2010, WBIR.

[2]  Tatiana V. Tkatchenko,et al.  Ketamine–xylazine anesthesia causes hyperopic refractive shift in mice , 2010, Journal of Neuroscience Methods.

[3]  Simon Tuohy,et al.  Depth-resolved wavefront aberrations using a coherence-gated Shack-Hartmann wavefront sensor. , 2010, Optics express.

[4]  David P. Biss,et al.  An adaptive-optics scanning laser ophthalmoscope for imaging murine retinal microstructure , 2010, BiOS.

[5]  David R Williams,et al.  In vivo imaging of microscopic structures in the rat retina. , 2009, Investigative ophthalmology & visual science.

[6]  Hermann Wagner,et al.  Spatial contrast sensitivity and grating acuity of barn owls. , 2009, Journal of vision.

[7]  Jessica I. W. Morgan,et al.  In vivo autofluorescence imaging of the human and macaque retinal pigment epithelial cell mosaic. , 2009, Investigative ophthalmology & visual science.

[8]  J. Crowston,et al.  Longitudinal profile of retinal ganglion cell damage after optic nerve crush with blue-light confocal scanning laser ophthalmoscopy. , 2008, Investigative ophthalmology & visual science.

[9]  Colin Bradley,et al.  A laser guide star wavefront sensor bench demonstrator for TMT. , 2008, Optics express.

[10]  M. K. Walsh,et al.  In vivo time-lapse fluorescence imaging of individual retinal ganglion cells in mice , 2008, Journal of Neuroscience Methods.

[11]  Hermann Wagner,et al.  Ocular aberrations in barn owl eyes , 2007, Vision Research.

[12]  C. Campbell.  Adaptive Optics in Vision Science , 2007 .

[13]  M. Campbell,et al.  Optical Quality of the Rat Eye , 2007 .

[14]  A. Hackam,et al.  In vivo three-dimensional high-resolution imaging of rodent retina with spectral-domain optical coherence tomography. , 2007, Investigative ophthalmology & visual science.

[15]  Alfredo Dubra,et al.  Wavefront sensor and wavefront corrector matching in adaptive optics. , 2007, Optics express.

[16]  Stephen A Burns,et al.  In vivo fluorescent imaging of the mouse retina using adaptive optics. , 2007, Optics letters.

[17]  Fabrice Manns,et al.  Simultaneous fundus imaging and optical coherence tomography of the mouse retina. , 2007, Investigative ophthalmology & visual science.

[18]  Maciej Wojtkowski,et al.  Noninvasive volumetric imaging and morphometry of the rodent retina with high-speed, ultrahigh-resolution optical coherence tomography. , 2006, Investigative ophthalmology & visual science.

[19]  Bernard P. Gee,et al.  In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells. , 2006, Optics express.

[20]  Susana Marcos,et al.  Optical aberrations in the mouse eye , 2006, Vision Research.

[21]  Michel Paques,et al.  High resolution fundus imaging by confocal scanning laser ophthalmoscopy in the mouse , 2006, Vision Research.

[22]  Edward N. Pugh,et al.  Physiological Features of the S- and M-cone Photoreceptors of Wild-type Mice from Single-cell Recordings , 2006, The Journal of general physiology.

[23]  Andreas Wenzel,et al.  In vivo confocal imaging of the retina in animal models using scanning laser ophthalmoscopy , 2005, Vision Research.

[24]  E. Irving,et al.  Refractive Error and Optical Image Quality in Three Strains of Albino Rats , 2005 .

[25]  P. Artal,et al.  Ocular aberrations as a function of wavelength in the near infrared measured with a femtosecond laser. , 2005, Optics express.

[26]  David Williams,et al.  Monochromatic ocular wavefront aberrations in the awake-behaving cat , 2004, Vision Research.

[27]  Gregory J. McCormick,et al.  Higher-order aberrations in eyes with irregular corneas after laser refractive surgery. , 2004, Ophthalmology.

[28]  Frank Schaeffel,et al.  A paraxial schematic eye model for the growing C57BL/6 mouse , 2004, Vision Research.

[29]  Robert W. Williams,et al.  Measurement of Refractive State and Deprivation Myopia in Two Strains of Mice , 2004, Optometry and vision science : official publication of the American Academy of Optometry.

[30]  Eric P.H. Yap,et al.  Two models of experimental myopia in the mouse , 2008, Vision Research.

[31]  R. Douglas,et al.  Developmental plasticity of mouse visual acuity , 2003, The European journal of neuroscience.

[32]  D. Lara-Saucedo,et al.  Aberrations of the Human Eye in Visible and Near Infrared Illumination , 2003, Optometry and vision science : official publication of the American Academy of Optometry.

[33]  P Artal,et al.  Dynamics of the eye's wave aberration. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[34]  H C Howland,et al.  Laboratory, Clinical, and Kindergarten Test of a New Eccentric Infrared Photorefractor (PowerRefractor) , 2000, Optometry and vision science : official publication of the American Academy of Optometry.

[35]  P Artal,et al.  Analysis of the performance of the Hartmann-Shack sensor in the human eye. , 2000, Journal of the Optical Society of America. A, Optics, image science, and vision.

[36]  L. Maffei,et al.  Behavioural visual acuity of wild type and bcl2 transgenic mouse , 1999, Vision Research.

[37]  R. Masland,et al.  The Major Cell Populations of the Mouse Retina , 1998, The Journal of Neuroscience.

[38]  J. Nathans,et al.  A Novel Signaling Pathway from Rod Photoreceptors to Ganglion Cells in Mammalian Retina , 1998, Neuron.

[39]  P Artal,et al.  Retinal image quality in the rodent eye , 1998, Visual Neuroscience.

[40]  D R Williams,et al.  Supernormal vision and high-resolution retinal imaging through adaptive optics. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[41]  Junzhong Liang,et al.  Aberrations and retinal image quality of the normal human eye. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[42]  A Roorda,et al.  Slope-based eccentric photorefraction: theoretical analysis of different light source configurations and effects of ocular aberrations. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[43]  A Roorda,et al.  Geometrical theory to predict eccentric photorefraction intensity profiles in the human eye. , 1995, Journal of the Optical Society of America. A, Optics, image science, and vision.

[44]  Junzhong Liang,et al.  Objective measurement of wave aberrations of the human eye with the use of a Hartmann-Shack wave-front sensor. , 1994, Journal of the Optical Society of America. A, Optics, image science, and vision.

[45]  A. Bradley,et al.  The chromatic eye: a new reduced-eye model of ocular chromatic aberration in humans. , 1992, Applied optics.

[46]  Terry Bossomaier,et al.  Optical image quality and the cone mosaic. , 1986, Science.

[47]  P. E. Hallett,et al.  A schematic eye for the mouse, and comparisons with the rat , 1985, Vision Research.

[48]  P. E. Hallett,et al.  Aspheric curvatures, refractive indices and chromatic aberration for the rat eye , 1983, Vision Research.

[49]  U. Dräger,et al.  Ganglion cell distribution in the retina of the mouse. , 1981, Investigative ophthalmology & visual science.

[50]  A. Hughes,et al.  A useful table of reduced schematic eyes for vertebrates which includes computed longitudinal chromatic aberrations , 1979, Vision Research.

[51]  A. Hughes.  The artefact of retinoscopy in the rat and rabbit eye has its origin at the retina/vitreous interface rather than in longitudinal chromatic aberration , 1979, Vision Research.

[52]  J. Sivak,et al.  Hypermetropia of small animals and chromatic aberration , 1978, Vision Research.

[53]  Allan W. Snyder,et al.  Information capacity of eyes , 1977, Vision Research.

[54]  G. Horridge.  The compound eye of insects , 1977 .

[55]  R. Muller,et al.  Real-time correction of atmospherically degraded telescope images through image sharpening , 1974 .

[56]  M. Millodot,et al.  Retinoscopy and Eye Size , 1970, Science.

[57]  D. G. Green,et al.  Optical and retinal factors affecting visual resolution. , 1965, The Journal of physiology.

[58]  Paul G. Roofe,et al.  The Vertebrate Visual System , 1958, Neurology.

[59]  David R Williams,et al.  In vivo imaging of the fine structure of rhodamine-labeled macaque retinal ganglion cells. , 2008, Investigative ophthalmology & visual science.

[60]  Young-Ho Lee,et al.  Vortex flow patterns of a heaving foil , 2006, J. Vis..

[61]  M. Seeliger,et al.  Grating acuity at different luminances in wild-type mice and in mice lacking rod or cone function. , 2005, Investigative ophthalmology & visual science.

[62]  P. de la Villa,et al.  Refractive changes induced by form deprivation in the mouse eye. , 2003, Investigative ophthalmology & visual science.

[63]  J. Pettigrew,et al.  Peak density and distribution of ganglion cells in the retinae of microchiropteran bats: implications for visual acuity. , 1988, Brain, behavior and evolution.

[64]  W. Andrew.  The vertebrate visual system , 1957 .