Comparison of intracytoplasmic sperm injection for inbred and hybrid mice

We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre‐implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post‐implantation development of the embryos from C57BL/6 females was significantly poorer than those of the eggs from hybrid females. Reciprocal crosses of C57BL/6 and B6C3F1 gametes revealed that the low rate of post‐implantation development of C57BL/6 embryos was due to oocyte factor(s), rather than the sperm factor. Mol. Reprod. Dev. 60: 74–78, 2001. © 2001 Wiley‐Liss, Inc.

[1]  R. Yanagimachi,et al.  A novel trans-complementation assay suggests full mammalian oocyte activation is coordinately initiated by multiple, submembrane sperm components. , 1999, Biology of Reproduction.

[2]  Y. Moriguchi,et al.  Relationship between Age-Related Decline in Fertility and In Vitro Fertilization Rate in IVCS Mice. , 1994 .

[3]  M. Yokoyama,et al.  Studies on the fertilization of mouse eggs in vitro , 1971 .

[4]  O. Ueda,et al.  Positive effect of partial zona-pellucida dissection on the in vitro fertilizing capacity of cryopreserved C57BL/6J transgenic mouse spermatozoa of low motility. , 1997, Biology of reproduction.

[5]  Y. Toyoda.  Studies on fertilization of mouse eggs in vitro. I. In vitro fertilization of eggs by fresh epididymal sperm , 1971 .

[6]  K. Jishage,et al.  Improved Embryo Transfer into the Oviduct by Local Application of a Vasoconstrictor in Mice , 1994 .

[7]  J. Catt,et al.  Comparative intracytoplasmic sperm injection (ICSI) in human and domestic species. , 1995, Reproduction, fertility, and development.

[8]  J. Shire,et al.  Genetic variation in the timing of first cleavage in mice: effect of paternal genotype. , 1980, Biology of Reproduction.

[9]  D. Solter,et al.  The beneficial effect EDTA on development of mouse one-cell embryos in chemically defined medium. , 1977, Developmental biology.

[10]  R. Yanagimachi,et al.  Normal mice develop from oocytes injected with spermatozoa with grossly misshapen heads. , 1996, Biology of reproduction.

[11]  R Yanagimachi,et al.  Mice cloned from embryonic stem cells. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[12]  R. Yanagimachi,et al.  Fertilization and development of mouse oocytes injected with isolated sperm heads. , 1996, Biology of reproduction.

[13]  S. Ratnam,et al.  Intracytoplasmic sperm injection of mouse oocytes with 5 mM Ca2+ at different intervals. , 1995, Human reproduction.

[14]  Maurizio Zuccotti,et al.  Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei , 1998, Nature.

[15]  R. Yanagimachi,et al.  Production of normal offspring from mouse oocytes injected with spermatozoa cryopreserved with or without cryoprotection. , 1998, Journal of reproduction and fertility.

[16]  S. Ng,et al.  Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa. , 1997, Human reproduction.

[17]  J. Biggers,et al.  Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM. , 1995, Biology of reproduction.

[18]  S. Ng,et al.  Sperm head decondensation, pronuclear formation, cleavage and embryonic development following intracytoplasmic injection of mitochondria-damaged sperm in mammals , 1997, Zygote.

[19]  Y. Toyoda,et al.  Fertilization and development in vitro of mouse eggs from inbred strains and f1 hybrids. , 1978 .

[20]  R Yanagimachi,et al.  Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material. , 1998, Biology of reproduction.

[21]  R. Yanagimachi,et al.  Inadequate function of sterile tw5/tw32 spermatozoa overcome by intracytoplasmic sperm injection , 1996, Molecular reproduction and development.

[22]  C. Markert.  Fertilization of mammalian eggs by sperm injection. , 1983, The Journal of experimental zoology.

[23]  R. Yanagimachi,et al.  An intact sperm nuclear matrix may be necessary for the mouse paternal genome to participate in embryonic development. , 1999, Biology of reproduction.

[24]  J. Biggers,et al.  Complete development in vitro of the pre-implantation stages of the mouse in a simple chemically defined medium. , 1968, Journal of reproduction and fertility.

[25]  R. Yanagimachi,et al.  Fertility of mouse spermatozoa retrieved from cadavers and maintained at 4°C , 1999 .

[26]  P. Devroey,et al.  Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte , 1992, The Lancet.

[27]  R Yanagimachi,et al.  Intracytoplasmic sperm injection in the mouse. , 1995, Biology of reproduction.

[28]  A. Trounson,et al.  Intracytoplasmic sperm injection in mice: increased fertilization and development to term after induction of the acrosome reaction. , 1995, Human reproduction.