Osteochondral repair using the combination of fibroblast growth factor and amorphous calcium phosphate/poly(L-lactic acid) hybrid materials.

A novel amorphous calcium phosphate (ACP)/poly(L-lactic acid) (PLLA) material, which can experience morphological variations in the microstructure is supposed to be a suitable candidate as scaffold for cartilage tissue-engineering. The purpose of this study was to evaluate the efficacy of this scaffold combined with basic fibroblast growth factor (bFGF) to repair articular cartilage defects in a rabbit model. Forty-two osteochondral defects created in the femoral condyles were (a) left untreated, (b) treated by PLLA combined with bFGF, or (c) ACP/PLLA loaded with bFGF. The treatment of PLLA incorporated with bFGF improved defect filling compared with that left untreated, while the regenerated tissue was mainly fibrocartilage and showed little bone formation with only a small amount of collagen type II (Col II) and no aggrecan gene message measured. When implanted with ACP/PLLA and bFGF, most of the defects were filled with a well-established layer of cartilage tissue with abundance of cartilaginous extracellular matrix accumulation observed. Positive immunohistochemical staining of Col II was observed. High levels of Col II and aggrecan message were also detected by RT-PCR. These results indicate the feasibility of using the combination of ACP/PLLA with bFGF for cartilage repair.

[1]  H J Mankin,et al.  Articular cartilage repair and transplantation. , 1998, Arthritis and rheumatism.

[2]  Peter Greil,et al.  Cellulose-based scaffold materials for cartilage tissue engineering. , 2006, Biomaterials.

[3]  G. Shen,et al.  Preparation of amorphous calcium phosphate in the presence of poly(ethylene glycol) , 2003 .

[4]  Magnus Lundberg,et al.  Treatment of osteochondral defects with autologous bone marrow in a hyaluronan-based delivery vehicle. , 2002, Tissue engineering.

[5]  Jiake Xu,et al.  Autologous chondrocyte implantation with collagen bioscaffold for the treatment of osteochondral defects in rabbits. , 2005, Tissue engineering.

[6]  L. Hangody,et al.  Autologous Osteochondral Mosaicplasty for the Treatment of Full-Thickness Defects of Weight-Bearing Joints: Ten Years of Experimental and Clinical Experience , 2003, The Journal of bone and joint surgery. American volume.

[7]  E. Morris,et al.  The Effect of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) on the Healing of Full-Thickness Defects of Articular Cartilage* , 1997, The Journal of bone and joint surgery. American volume.

[8]  S W O'Driscoll,et al.  Durability of regenerated articular cartilage produced by free autogenous periosteal grafts in major full-thickness defects in joint surfaces under the influence of continuous passive motion. A follow-up report at one year. , 1988, The Journal of bone and joint surgery. American volume.

[9]  E. Itoi,et al.  Effects of intraarticular administration of basic fibroblast growth factor with hyaluronic acid on osteochondral defects of the knee in rabbits , 2005, Archives of Orthopaedic and Trauma Surgery.

[10]  Hitoshi Hirata,et al.  Enhanced repair of large osteochondral defects using a combination of artificial cartilage and basic fibroblast growth factor. , 2005, Biomaterials.

[11]  L. Hangody,et al.  Experimental results of donor site filling for autologous osteochondral mosaicplasty. , 2003, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[12]  Hideki Yoshikawa,et al.  A new biotechnology for articular cartilage repair: subchondral implantation of a composite of interconnected porous hydroxyapatite, synthetic polymer (PLA-PEG), and bone morphogenetic protein-2 (rhBMP-2). , 2005, Osteoarthritis and cartilage.

[13]  Qiang Zhao,et al.  Repair of large articular cartilage defects with implants of autologous mesenchymal stem cells seeded into beta-tricalcium phosphate in a sheep model. , 2004, Tissue engineering.

[14]  Arnold I Caplan,et al.  Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable calcium phosphate and hyaluronan sponge. , 2002, Tissue engineering.

[15]  Koji Hattori,et al.  Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. , 2005, Biomaterials.

[16]  K Remberger,et al.  Enhanced repair of articular cartilage defects in vivo by transplanted chondrocytes overexpressing insulin-like growth factor I (IGF-I) , 2005, Gene Therapy.

[17]  A. Grodzinsky,et al.  Effects of a cultured autologous chondrocyte‐seeded type II collagen scaffold on the healing of a chondral defect in a canine model , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[18]  Lars Engebretsen,et al.  Autologous chondrocyte implantation compared with microfracture in the knee. A randomized trial. , 2004, The Journal of bone and joint surgery. American volume.

[19]  S. Woo,et al.  Finite Element Analyses of Repaired Articular Surfaces , 1991, Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine.

[20]  C B Sledge,et al.  Chondrocyte-seeded collagen matrices implanted in a chondral defect in a canine model. , 1998, Biomaterials.

[21]  M. Ochi,et al.  Beneficial effect of basic fibroblast growth factor on the repair of full-thickness defects in rabbit articular cartilage , 1999, Archives of Orthopaedic and Trauma Surgery.

[22]  W. Richter,et al.  An in vivo study of a growth-factor enhanced, cell free, two-layered collagen-tricalcium phosphate in deep osteochondral defects. , 2006, Biomaterials.

[23]  R. Tuan,et al.  A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. , 2005, Biomaterials.

[24]  M D McKee,et al.  Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle. , 2005, Osteoarthritis and cartilage.

[25]  Steven B Cohen,et al.  Transforming growth factor-beta in calcium alginate beads for the treatment of articular cartilage defects in the rabbit. , 2002, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[26]  Antonios G Mikos,et al.  Osteochondral repair in the rabbit model utilizing bilayered, degradable oligo(poly(ethylene glycol) fumarate) hydrogel scaffolds. , 2005, Journal of biomedical materials research. Part A.

[27]  G. Shen,et al.  Preparation, characterization and cytocompatibility of porous ACP/PLLA composites. , 2006, Journal of biomedical materials research. Part A.

[28]  A. Sudo,et al.  Biological performance of a three-dimensional fabric as artificial cartilage in the repair of large osteochondral defects in rabbit. , 1999, Biomaterials.

[29]  M. Nimni.  Polypeptide growth factors: targeted delivery systems. , 1997, Biomaterials.

[30]  Mark A. Randolph,et al.  Tissue Engineered Neocartilage Using Plasma Derived Polymer Substrates and Chondrocytes , 1998, Plastic and reconstructive surgery.

[31]  P. Thomsen,et al.  Leucocyte accumulation and leukotriene B4 release in response to polyglactin 910 and expanded polytetrafluoroethylene in hollow chambers in the rat. , 1995, Biomaterials.

[32]  M J Glimcher,et al.  Cell origin and differentiation in the repair of full-thickness defects of articular cartilage. , 1993, The Journal of bone and joint surgery. American volume.

[33]  R P Jakob,et al.  Articular cartilage repair using a tissue-engineered cartilage-like implant: an animal study. , 2001, Osteoarthritis and cartilage.

[34]  Y. Sugioka,et al.  Basic fibroblast growth factor stimulates articular cartilage enlargement in young rats in vivo , 1996, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[35]  M. Brittberg,et al.  Treatment of Osteochondritis Dissecans of the Knee with Autologous Chondrocyte Transplantation: Results at Two to Ten Years , 2003, The Journal of bone and joint surgery. American volume.

[36]  G D Smith,et al.  A clinical review of cartilage repair techniques. , 2005, The Journal of bone and joint surgery. British volume.

[37]  H. Mankin,et al.  The response of articular cartilage to mechanical injury. , 1982, The Journal of bone and joint surgery. American volume.

[38]  R. Giardino,et al.  Transplantation of chondrocytes seeded on a hyaluronan derivative (hyaff-11) into cartilage defects in rabbits. , 2001, Biomaterials.

[39]  Su-Hyang Kim,et al.  Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer. , 2004, Biomaterials.

[40]  T. Aigner,et al.  Autologous Chondrocyte Implantation and Osteochondral Cylinder Transplantation in Cartilage Repair of the Knee Joint: A Prospective, Comparative Trial , 2003, The Journal of bone and joint surgery. American volume.