Cloning and Characterization of the Gene Encoding HMGS Synthase in Polygonatum sibiricum

The saponins of Polygonatum sibiricum had many pharmacological activities such as antitumor, antioxidation, and blood sugar lowering, which were synthesized by two pathways: mevalonate (MVA) and methylerythritol phosphate (MEP). 3-Hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) was the key enzyme in the MVA synthesis pathway, and its expression level may affect the accumulation of saponins which were the main active ingredients of P. sibiricum. In this study, we successfully cloned HMGS1 and HMGS2 from P. sibiricum and their sequence similarity was 93.71% with 89 different sites. The multiple sequence alignment results indicated that the N-terminal sequences of HMGS were conserved. Phylogenetic analysis showed that P. sibiricum, A. officinalis, N. tazetta, D. nobile, and other relatives had a common evolutionary ancestor. The expression levels of both HMGSs and the total saponin content in different tissues revealed that HMGS expression in rhizomes was positively correlated with total saponin content. Further study of the abiotic stress effect of Methyl Jasmonate (MeJA) demonstrated that the expression of HMGS1 and HMGS2 genes was induced by MeJA, peaked at 24 h, and fell by 48 h. Our present findings would provide a blueprint for future studies of HMGS and its role in triterpenoid biosynthesis in P. sibiricum.

[1]  S. Chen,et al.  De novo assembly and analysis of Polygonatum cyrtonema Hua and identification of genes involved in polysaccharide and saponin biosynthesis , 2022, BMC Genomics.

[2]  L. Rahman,et al.  Molecular cloning and characterization of Triterpenoid Biosynthetic Pathway Gene HMGS in Centella asiatica (Linn.) , 2022, Molecular Biology Reports.

[3]  Haifeng Yan,et al.  Cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and 3-hydroxy-3-methylglutaryl-CoA synthase genes in Santalum album , 2021, Scientific Reports.

[4]  Dongfeng Yang,et al.  Application of 1H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib , 2020, Planta.

[5]  So-Young Park,et al.  Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures , 2020, International journal of molecular sciences.

[6]  M. Sharifi,et al.  Time-dependent behavior of phenylpropanoid pathway in response to methyl jasmonate in Scrophularia striata cell cultures , 2019, Plant Cell Reports.

[7]  Qi Zhang,et al.  Molecular mechanism and inhibitory targets of dioscin in HepG2 cells. , 2018, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[8]  Weiwei Zhang,et al.  Cloning, Expression Profiling and Functional Analysis of CnHMGS, a Gene Encoding 3-hydroxy-3-Methylglutaryl Coenzyme A Synthase from Chamaemelum nobile , 2016, Molecules.

[9]  F. Xu,et al.  RESEARCH ADVANCE OF 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A SYNTHASE IN PLANT ISOPRENOID BIOSYNTHESIS , 2015 .

[10]  P. Su,et al.  Cloning and Characterisation of the Gene Encoding 3-Hydroxy-3-Methylglutaryl-CoA Synthase in Tripterygium wilfordii , 2014, Molecules.

[11]  M. Chye,et al.  Transgenic Tobacco Overexpressing Brassica juncea HMG-CoA Synthase 1 Shows Increased Plant Growth, Pod Size and Seed Yield , 2014, PloS one.

[12]  Zhu Hong-ta.  Effects of Methy Jasmonate on the Content of Total Saponin in Tissue Culture Seedlings of Panax notoginseng , 2014 .

[13]  R. Rawat,et al.  Overexpression of Brassica juncea wild-type and mutant HMG-CoA synthase 1 in Arabidopsis up-regulates genes in sterol biosynthesis and enhances sterol production and stress tolerance. , 2012, Plant biotechnology journal.

[14]  Yu Zu-wen.  The Extraction and Eetermination of Polyploid Main Active Ingredients of Polygonatum , 2011 .

[15]  L. Qin,et al.  Methyl jasmonate induces ganoderic acid biosynthesis in the basidiomycetous fungus Ganoderma lucidum. , 2010, Bioresource technology.

[16]  S. Ishiguro,et al.  The Arabidopsis FLAKY POLLEN1 gene encodes a 3-hydroxy-3-methylglutaryl-coenzyme A synthase required for development of tapetum-specific organelles and fertility of pollen grains. , 2010, Plant & cell physiology.

[17]  L. Zhang,et al.  Molecular cloning and expression analyses of a new gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Taxus × media , 2006, Biologia Plantarum.

[18]  H. Uk.  Advances on the Plant Terpenoid Isoprenoid Biosynthetic Pathway and Its Key Enzymes , 2006 .

[19]  A. Hemmerlin,et al.  Brassica juncea HMG-CoA synthase: localization of mRNA and protein , 2005, Planta.

[20]  T. Bach,et al.  Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive. , 2000, The Plant journal : for cell and molecular biology.