Enrichment and identification of bacteria capable of reducing chemical oxygen demand of anaerobically treated molasses spent wash

Aims:  The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash.

[1]  W. Wade,et al.  Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA , 1998, Applied and Environmental Microbiology.

[2]  R. Fani,et al.  Molecular characterization of an n-alkane-degrading bacterial community and identification of a new species, Acinetobacter venetianus. , 1997, Research in microbiology.

[3]  M. Nei,et al.  MEGA: Molecular Evolutionary Genetics Analysis, Version 1.02. , 1995 .

[4]  R Marchant,et al.  Biological treatment of distillery waste for pollution‐remediation , 1995, Journal of basic microbiology.

[5]  S. Goodison,et al.  16S ribosomal DNA amplification for phylogenetic study , 1991, Journal of bacteriology.

[6]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[7]  G. D. Di Giovanni,et al.  Fingerprinting of Mixed Bacterial Strains and BIOLOG Gram-Negative (GN) Substrate Communities by Enterobacterial Repetitive Intergenic Consensus Sequence-PCR (ERIC-PCR) , 1999, Current Microbiology.

[8]  D. Washington,et al.  Standard Methods for the Examination of Water and Wastewater , 1971 .

[9]  D. G. Allen,et al.  Phenotypic fingerprinting of microbial communities in wastewater treatment systems , 1996 .

[10]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[11]  Peter H. A. Sneath,et al.  Numerical Taxonomy: The Principles and Practice of Numerical Classification , 1973 .

[12]  J. Tiedje,et al.  High Levels of Endemicity of 3-Chlorobenzoate-Degrading Soil Bacteria , 1998, Applied and Environmental Microbiology.

[13]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[14]  A. Tripathi,et al.  Treatment of anaerobically digested distillery spentwash in a two-stage bioreactor using Pseudomonas putida and Aeromonas sp. , 2002 .

[15]  F. Corpet Multiple sequence alignment with hierarchical clustering. , 1988, Nucleic acids research.

[16]  R. Fani,et al.  A molecular strategy for the study of natural bacterial communities by PCR-based techniques , 1996 .

[17]  B L Maidak,et al.  The RDP-II (Ribosomal Database Project) , 2001, Nucleic Acids Res..

[18]  M. Alexander,et al.  Biodegradation of chemicals of environmental concern. , 1981, Science.

[19]  John Walker,et al.  A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae , 1992, Nucleic Acids Res..

[20]  S. E. Lantz,et al.  Phylogenetic and physiological comparisons of PAH-degrading bacteria from geographically diverse soils , 1997, Antonie van Leeuwenhoek.