Molecular Characterization of Multidrug Resistance in Streptococcus mitis

ABSTRACT Antimicrobial resistance was characterized for 14 strains ofStreptococcus mitis. HinfI restriction fragment length mapping of gyrA PCR amplicons from three ciprofloxacin-resistant isolates correlated with mutations associated with such resistance in other organisms. By using PCR, seven erythromycin-resistant strains were found to possess either the mef or ermB gene. Hybridization revealed tet(M) in seven tetracycline-resistant isolates.

[1]  M. Avison,et al.  Interscience Conference on Antimicrobial Agents and Chemotherapy , 2001 .

[2]  Mary Jane Ferraro,et al.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically : approved standard , 2000 .

[3]  T. Grebe,et al.  Detection of erythromycin-resistant determinants by PCR , 1996, Antimicrobial agents and chemotherapy.

[4]  J. Ambler,et al.  Involvement of topoisomerase IV and DNA gyrase as ciprofloxacin targets in Streptococcus pneumoniae , 1996, Antimicrobial agents and chemotherapy.

[5]  M. Ferraro,et al.  Emergence of high rates of antimicrobial resistance among viridans group streptococci in the United States , 1996, Antimicrobial agents and chemotherapy.

[6]  F. Tenover,et al.  Detection of tet(M) and tet(O) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease. , 1995, Oral microbiology and immunology.

[7]  L. Peterson,et al.  Ciprofloxacin resistance in coagulase-positive and -negative staphylococci: role of mutations at serine 84 in the DNA gyrase A protein of Staphylococcus aureus and Staphylococcus epidermidis , 1991, Antimicrobial Agents and Chemotherapy.

[8]  E. Potgieter,et al.  Reciprocal transfer of penicillin resistance genes between Streptococcus pneumoniae, Streptococcus mitior and Streptococcus sanguis. , 1991, The Journal of antimicrobial chemotherapy.

[9]  J. Lupski,et al.  Cloning and nucleotide sequence of a chromosomally encoded tetracycline resistance determinant, tetA(M), from a pathogenic, methicillin-resistant strain of Staphylococcus aureus , 1990, Antimicrobial Agents and Chemotherapy.

[10]  D. Clermont,et al.  Identification of chromosomal antibiotic resistance genes in Streptococcus anginosus ("S. milleri") , 1990, Antimicrobial Agents and Chemotherapy.

[11]  G. Weinstock,et al.  Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites , 1990, Journal of clinical microbiology.

[12]  B. Spratt,et al.  Penicillin-resistant viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococcus pneumoniae. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Waitz Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically , 1990 .

[14]  A. Coykendall Classification and identification of the viridans streptococci , 1989, Clinical Microbiology Reviews.

[15]  S. Levy,et al.  Energy-dependent efflux mediated by class L (tetL) tetracycline resistance determinant from streptococci , 1987, Antimicrobial Agents and Chemotherapy.

[16]  M. Weinstein,et al.  Prevalence of macrolides-lincosamides-streptogramin B resistance and erm gene classes among clinical strains of staphylococci and streptococci , 1987, Antimicrobial Agents and Chemotherapy.

[17]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.