论文信息 - Localization of a specific nucleotide in yeast tRNA by scanning transmission electron microscopy using an undecagold cluster.

Localization of a specific nucleotide in yeast tRNA by scanning transmission electron microscopy using an undecagold cluster.

Scanning transmission electron microscopic images of transfer RNAs reveal the molecular dimensions and compact morphology of these small macromolecules in unprecedented detail. Selective labeling of a sulfhydryl group on 2-thiocytidine enzymatically inserted at position 75 at the 3' end of yeast tRNA(Phe) with an undecagold cluster permits identification of this specific tRNA site by dark field STEM. Imaging of a single nucleotide at a defined location on the tRNA molecule should make it possible to localize in situ tRNAs at the A, P, and E sites of the ribosomal peptidyl transferase center, and in complexes of tRNA with enzymes and elongation factors. In addition, this approach may be used for the highly specific topographical mapping of other RNAs and/or biological macromolecular complexes.

[1] S. Colowick,et al. Methods in Enzymology , Vol , 1966 .

[2] Synthesis of water-soluble undecagold cluster compounds of potential importance in electron microscopic and other studies of biological systems , 1978 .

[3] N. Seeman,et al. Three-Dimensional Tertiary Structure of Yeast Phenylalanine Transfer RNA , 1974, Science.

[4] P. Frey,et al. Synthesis of undecagold cluster molecules as biochemical labeling reagents. 2. Bromoacetyl and maleimido undecagold clusters. , 1984, Biochemistry.

[5] B. Clark,et al. Structure of yeast phenylalanine tRNA at 3 Å resolution , 1974, Nature.

[6] J L Sussman,et al. Crystal structure of yeast phenylalanine transfer RNA. I. Crystallographic refinement. , 1978, Journal of molecular biology.

[7] J. Hainfeld,et al. Biospecific labeling with undecagold: visualization of the biotin-binding site on avidin. , 1982, Science.

[8] F. Cramer,et al. Preparation in vitro of a 2-thiocytidine-containing yeast tRNA Phe -A 73 -C 74 -S 2 C 75 -A 76 and its interaction wiith p-hydroxymercuribenzoate. , 1973, European journal of biochemistry.