Use of ICP and XAS to determine the enhancement of gold phytoextraction by Chilopsis linearis using thiocyanate as a complexing agent

Under natural conditions gold has low solubility that reduces its bioavailability, a critical factor for phytoextraction. Researchers have found that phytoextraction can be improved by using synthetic chelating agents. Preliminary studies have shown that desert willow (Chilopsis linearis), a common inhabitant of the Chihuahuan Desert, is able to extract gold from a gold-enriched medium. The objective of the present study was to determine the ability of thiocyanate to enhance the gold-uptake capacity of C. linearis. Seedlings of this plant were exposed to the following hydroponics treatment: (1) 5 mg Au L−1 (2.5×10−5 mol L−1), (2) 5 mg Au L−1+10−5 mol L−1 NH4SCN, (3) 5 mg Au L−1+5×10−5 mol L−1 NH4SCN, and (4) 5 mg Au L−1+10−4 mol L−1 NH4SCN. Each treatment had its respective control. After 2 weeks we determined the effect of the treatment on plant growth and gold content by inductively coupled plasma–optical emission spectroscopy (ICP–OES). No signs of shoot-growth inhibition were observed at any NH4SCN treatment level. The ICP–OES analysis showed that addition of 10−4 mol L−1 NH4SCN increased the concentration of gold by about 595, 396, and 467% in roots, stems, and leaves, respectively. X-ray absorption spectroscopy (XAS) studies showed that the oxidation state of gold was Au(0) and that gold nanoparticles were formed inside the plants.

[1]  Daniel Hammer,et al.  Root development and heavy metal phytoextraction efficiency: comparison of different plant species in the field , 2003, Plant and Soil.

[2]  Yih-Fen Wu,et al.  Effects of Ammonium Thiocyanate on Carbohydrate Metabolism in the Cotton Plant , 1969, Weed Science.

[3]  R. Chaney,et al.  Developing commercial phytoextraction technologies : practical considerations , 2001 .

[4]  P. J. Peterson,et al.  Gold uptake by perennial ryegrass: The influence of humates on the cycling of gold in soils , 1989 .

[5]  T. Ressler WinXAS: a program for X-ray absorption spectroscopy data analysis under MS-Windows. , 1998, Journal of synchrotron radiation.

[6]  J. Peralta-Videa,et al.  Alfalfa growth promotion by bacteria grown under iron limiting conditions , 2002 .

[7]  R. Simcock,et al.  Gold Uptake by Plants , 1999 .

[8]  S. Pavlostathis,et al.  Aerobic biodegradation of thiocyanate , 1997 .

[9]  J. Gardea-Torresdey,et al.  Characterization of Cr(VI) binding and reduction to Cr(III) by the agricultural byproducts of Avena monida (oat) biomass. , 2000, Journal of hazardous materials.

[10]  R. Simcock,et al.  Harvesting a crop of gold in plants , 1998, Nature.

[11]  Jose R. Peralta-Videa,et al.  Formation and Growth of Au Nanoparticles inside Live Alfalfa Plants , 2002 .

[12]  J. Gardea-Torresdey,et al.  Uptake and Effects of Five Heavy Metals on Seed Germination and Plant Growth in Alfalfa (Medicago sativa L.) , 2001, Bulletin of environmental contamination and toxicology.

[13]  A. Ankudinov,et al.  REAL-SPACE MULTIPLE-SCATTERING CALCULATION AND INTERPRETATION OF X-RAY-ABSORPTION NEAR-EDGE STRUCTURE , 1998 .

[14]  S. McGrath,et al.  Leaching of heavy metals from contaminated soils using EDTA. , 2001, Environmental pollution.

[15]  J. Peralta-Videa,et al.  Effect of mixed cadmium, copper, nickel and zinc at different pHs upon alfalfa growth and heavy metal uptake. , 2002, Environmental pollution.

[16]  J. Gardea-Torresdey,et al.  ENVIRONMENTAL AND BIOLOGICAL APPLICATIONS OF EXTENDED X-RAY ABSORPTION FINE STRUCTURE (EXAFS) AND X-RAY ABSORPTION NEAR EDGE STRUCTURE (XANES) SPECTROSCOPIES , 2002 .

[17]  M. Mergeay,et al.  Genetic engineering in the improvement of plants for phytoremediation of metal polluted soils. , 2000, Environmental pollution.

[18]  J. Gardea-Torresdey,et al.  Uptake and Effects of Five Heavy Metals on Seed Germination and Plant Growth in Alfalfa ( L.) , 2001 .

[19]  I. Raskin,et al.  Use of plant roots for phytoremediation and molecular farming. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[20]  L. Furenlid,et al.  Reduction and Accumulation of Gold(III) by Medicago sativa Alfalfa Biomass: X-ray Absorption Spectroscopy, pH, and Temperature Dependence. , 2000, Environmental science & technology.

[21]  B. Ravel,et al.  ATOMS: crystallography for the X-ray absorption spectroscopist. , 2001, Journal of synchrotron radiation.

[22]  M. Borowski Size Determination of Small Cu-Clusters by EXAFS , 1997 .

[23]  J. Peralta-Videa,et al.  Uptake and reduction of Cr(VI) to Cr(III) by mesquite (Prosopis spp.): chromate-plant interaction in hydroponics and solid media studied using XAS. , 2003, Environmental science & technology.

[24]  P. J. Peterson,et al.  Gold in plants , 1980 .

[25]  C. Anderson,et al.  Chemically-induced uptake of gold by root crops: Its significance for phytomining , 2000 .

[26]  A. Piccolo,et al.  Potential availability of heavy metals to phytoextraction from contaminated soils induced by exogenous humic substances. , 2003, Chemosphere.

[27]  B. Kos,et al.  EDTA enhanced heavy metal phytoextraction: metal accumulation, leaching and toxicity , 2001, Plant and Soil.

[28]  R. Brooks Biological methods of prospecting for gold , 1982 .

[29]  P. Santamaria,et al.  Ammonium and nitrate influence on artichoke growth rate and uptake of inorganic ions , 1996 .