论文信息 - Ebola virus disease in the Democratic Republic of Congo.

Ebola virus disease in the Democratic Republic of Congo.

BACKGROUND The seventh reported outbreak of Ebola virus disease (EVD) in the equatorial African country of the Democratic Republic of Congo (DRC) began on July 26, 2014, as another large EVD epidemic continued to spread in West Africa. Simultaneous reports of EVD in equatorial and West Africa raised the question of whether the two outbreaks were linked. METHODS We obtained data from patients in the DRC, using the standard World Health Organization clinical-investigation form for viral hemorrhagic fevers. Patients were classified as having suspected, probable, or confirmed EVD or a non-EVD illness. Blood samples were obtained for polymerase-chain-reaction-based diagnosis, viral isolation, sequencing, and phylogenetic analysis. RESULTS The outbreak began in Inkanamongo village in the vicinity of Boende town in Équateur province and has been confined to that province. A total of 69 suspected, probable, or confirmed cases were reported between July 26 and October 7, 2014, including 8 cases among health care workers, with 49 deaths. As of October 7, there have been approximately six generations of cases of EVD since the outbreak began. The reported weekly case incidence peaked in the weeks of August 17 and 24 and has since fallen sharply. Genome sequencing revealed Ebola virus (EBOV, Zaire species) as the cause of this outbreak. A coding-complete genome sequence of EBOV that was isolated during this outbreak showed 99.2% identity with the most closely related variant from the 1995 outbreak in Kikwit in the DRC and 96.8% identity to EBOV variants that are currently circulating in West Africa. CONCLUSIONS The current EVD outbreak in the DRC has clinical and epidemiologic characteristics that are similar to those of previous EVD outbreaks in equatorial Africa. The causal agent is a local EBOV variant, and this outbreak has a zoonotic origin different from that in the 2014 epidemic in West Africa. (Funded by the Centre International de Recherches Médicales de Franceville and others.).

[1] W. Team. Ebola Virus Disease in West Africa — The First 9 Months of the Epidemic and Forward Projections , 2014 .

[2] Rachel S. G. Sealfon,et al. Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak , 2014, Science.

[3] Stephan Günther,et al. Emergence of Zaire Ebola virus disease in Guinea. , 2014, The New England journal of medicine.

[4] W. Ian Lipkin,et al. Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda , 2008, PLoS pathogens.

[5] G. Kennedy,et al. Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR , 2008, BMC Molecular Biology.

[6] M. Nei,et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. , 2011, Molecular biology and evolution.

[7] Christopher Dye,et al. The international Ebola emergency. , 2014, The New England journal of medicine.

[8] Sergey I. Nikolenko,et al. SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing , 2012, J. Comput. Biol..

[9] John P. Huelsenbeck,et al. MrBayes 3: Bayesian phylogenetic inference under mixed models , 2003, Bioinform..

[10] Mikiko Senga,et al. Ebola virus disease in West Africa--the first 9 months of the epidemic and forward projections. , 2014, The New England journal of medicine.