Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria

Information on mosquito-borne filarioid helminths in Austria is scarce, but recent discoveries of Dirofilaria repens indicate autochthonous distribution of this parasite in Eastern Austria. In the current xenomonitoring study, more than 48,000 mosquitoes were collected in Eastern Austria between 2013 and 2015, using different sampling techniques and storage conditions, and were analysed in pools with molecular tools for the presence of filarioid helminth DNA. Overall, DNA of D. repens, Setaria tundra, and two unknown filarioid helminths were documented in twenty mosquito pools within the mitochondrial cox1 gene (barcode region). These results indicate that S. tundra, with roe deer as definite hosts, is common in Eastern Austria, with most occurrences in floodplain mosquitoes (e.g., Aedes vexans). Moreover, DNA of D. repens was found in an Anopheles plumbeus mosquito close to the Slovakian border, indicating that D. repens is endemic in low prevalence in Eastern Austria. This study shows that xenomonitoring is an adequate tool to analyse the presence of filarioid helminths, but results are influenced by mosquito sampling techniques, storage conditions, and molecular protocols.

[1]  M. Miterpáková,et al.  Human Dirofilaria repens infections diagnosed in Slovakia in the last 10 years (2007–2017) , 2017, Wiener klinische Wochenschrift.

[2]  J. Votýpka,et al.  Mosquitoes in the Danube Delta: searching for vectors of filarioid helminths and avian malaria , 2017, Parasites & Vectors.

[3]  Simon Vitecek,et al.  Landscape structure affects distribution of potential disease vectors (Diptera: Culicidae) , 2017, Parasites & Vectors.

[4]  Alexander Mathis,et al.  Development of Dirofilaria immitis and Dirofilaria repens in Aedes japonicus and Aedes geniculatus , 2017, Parasites & Vectors.

[5]  M. Chriel,et al.  Detection and molecular characterization of the mosquito-borne filarial nematode Setaria tundra in Danish roe deer (Capreolus capreolus) , 2017, International journal for parasitology. Parasites and wildlife.

[6]  Á. Bede‐Fazekas,et al.  Impacts of urbanisation level and distance from potential natural mosquito breeding habitats on the abundance of canine dirofilariosis. , 2016, Acta veterinaria Hungarica.

[7]  H. Auer,et al.  Dirofilaria in Humans, Dogs, and Vectors in Austria (1978–2014)—From Imported Pathogens to the Endemicity of Dirofilaria repens , 2016, PLoS neglected tropical diseases.

[8]  D. Modrý,et al.  Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports) , 2016, BMC Infectious Diseases.

[9]  L. Szeredi,et al.  Retrospective study on the distribution of Dirofilaria immitis in dogs in Hungary. , 2016, Veterinary parasitology.

[10]  V. Čabanová,et al.  Canine dirofilariosis endemic in Central Europe—10 years of epidemiological study in Slovakia , 2016, Parasitology Research.

[11]  S. Klimpel,et al.  Direct PCR of indigenous and invasive mosquito species: a time‐ and cost‐effective technique of mosquito barcoding , 2015, Medical and veterinary entomology.

[12]  W. Rożej-Bielicka,et al.  Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions? , 2016, Parasitology Research.

[13]  R. Herczeg,et al.  Circulation of Dirofilaria repens, Setaria tundra, and Onchocercidae species in Hungary during the period 2011-2013. , 2015, Veterinary parasitology.

[14]  E. Bocková,et al.  Potential mosquito (Diptera:Culicidae) vector of Dirofilaria repens and Dirofilaria immitis in urban areas of Eastern Slovakia , 2015, Parasitology Research.

[15]  A. Alić,et al.  A molecular survey of vector-borne pathogens in red foxes (Vulpes vulpes) from Bosnia and Herzegovina , 2015, Parasites & Vectors.

[16]  H. Fuehrer,et al.  Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria , 2015, Parasites & Vectors.

[17]  F. Rubel,et al.  Mosquitoes (Diptera: Culicidae) and their relevance as disease vectors in the city of Vienna, Austria , 2014, Parasitology Research.

[18]  P. Hufnagl,et al.  Autochthonous Dirofilaria repens in Austria , 2014, Parasites & Vectors.

[19]  N. Becker,et al.  Stable Transmission of Dirofilaria repens Nematodes, Northern Germany , 2014, Emerging infectious diseases.

[20]  H. Kampen,et al.  Molecular detection of Dirofilaria immitis, Dirofilaria repens and Setaria tundra in mosquitoes from Germany , 2014, Parasites & Vectors.

[21]  Z. Hubálek,et al.  Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia , 2013, Parasitology Research.

[22]  J. González-Miguel,et al.  Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic , 2012, Clinical Microbiology Reviews.

[23]  N. Becker,et al.  Molecular detection of Setaria tundra (Nematoda: Filarioidea) and an unidentified filarial species in mosquitoes in Germany , 2012, Parasites & Vectors.

[24]  C. Genchi,et al.  Dirofilarial infections in Europe. , 2011, Vector borne and zoonotic diseases.

[25]  A. Joachim,et al.  [Detection of Dirofilaria in Austrian dogs]. , 2009, Berliner und Munchener tierarztliche Wochenschrift.

[26]  R. Kortet,et al.  Vectors and transmission dynamics for Setaria tundra (Filarioidea; Onchocercidae), a parasite of reindeer in Finland , 2009, Parasites & Vectors.

[27]  R. Kortet,et al.  Setaria tundra microfilariae in reindeer and other cervids in Finland , 2008, Parasitology Research.

[28]  A. Oksanen,et al.  Outbreak of parasitic peritonitis in reindeer in Finland , 2007, Veterinary Record.

[29]  K. Büttner.  Untersuchungen zur Parasitierung des Rehwildes bei steigendem Jagddruck , 1978, Zeitschrift für Jagdwissenschaft.

[30]  P. Deplazes,et al.  [Is dirofilariasis in dogs spreading in south Switzerland?]. , 1998, Schweizer Archiv fur Tierheilkunde.

[31]  P. Deplazes,et al.  [Endoparasite infection in stray and abandoned dogs in southern Switzerland]. , 1995, Schweizer Archiv fur Tierheilkunde.