Two diverged human homeobox genes involved in the differentiation of human hematopoietic progenitors map to chromosome I, bands q41–42.I

Proteins encoded by homeobox containing genes are sequence‐specific DNA binding proteins implicated in the control of gene expression in both developing and adult tissues. Two recently characterized human homeobox genes, HB9 and HB24, are highly expressed in CD34‐positive marrow cells but not in CD34‐depleted marrow cells, Their expression is readily down‐regulated during the differentiation of hematopoietic progenitors to specific cell lineages. In this study, genomic DNA fragments isolated with HB9 (3 kb) and HB24 (6 kb) cDNAs were used to map their chromosomal location by fluorescence in situ hybridization. Both HB9 and HB24 DNA probes gave specific hybridization signals on chromosome 1. The hybridization loci were identified by combining fluorescence images of the probe signals with fluorescence banding patterns generated by cohybridization in situ with an Alu probe (R‐like banding) and by DAPI staining (G‐like). The results demonstrate that the loci of the HB24 and HB9 genes are within bands 1q41–q42.1. A cohybridization experiment utilizing both probes with two‐color fluorescence imaging could not resolve separate loci for the two genes. © 1992 Wiley‐Liss, Inc.

[1]  F. Behm,et al.  Different Molecular Consequences of the 1;19 Chromosomal Translocation in Childhood B - Cell Precursor Acute Lymphoblastic Leukemia , 1992 .

[2]  D. Ward,et al.  The gene for human erythrocyte protein 4.2 maps to chromosome 15q15. , 1992, American journal of human genetics.

[3]  J. Kehrl,et al.  Selective expression of two homeobox genes in CD34-positive cells from human bone marrow. , 1991, Blood.

[4]  Y. Deguchi,et al.  Nucleotide sequence of a novel diverged human homeobox gene encodes a DNA binding protein , 1991, Nucleic Acids Res..

[5]  D. Ward,et al.  In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies. , 1991, Genomics.

[6]  B. Trask,et al.  Mapping of human chromosome Xq28 by two-color fluorescence in situ hybridization of DNA sequences to interphase cell nuclei. , 1991, American journal of human genetics.

[7]  J. Mcneil,et al.  Interphase and metaphase resolution of different distances within the human dystrophin gene. , 1990, Science.

[8]  David Baltimore,et al.  A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B all , 1990, Cell.

[9]  Michael L. Cleary,et al.  Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor , 1990, Cell.

[10]  G Hermanson,et al.  High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. , 1990, Science.

[11]  M. D'Esposito,et al.  The human HOX gene family. , 1989, Nucleic acids research.

[12]  R. Cortese,et al.  The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain , 1989, Cell.

[13]  E. D. De Robertis,et al.  Vertebrate homeodomain proteins: families of region-specific transcription factors. , 1989, Trends in biochemical sciences.

[14]  W. Schaffner,et al.  A cloned octamer transcription factor stimulates transcription from lymphoid–specific promoters in non–B cells , 1988, Nature.

[15]  L. Sachs,et al.  DNA rearrangement of a homeobox gene in myeloid leukaemic cells. , 1988, The EMBO journal.

[16]  M. Rosenfeld,et al.  A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype , 1988, Cell.

[17]  L. Staudt,et al.  A human protein specific for the immunoglobulin octamer DNA motif contains a functional homeobox domain , 1988, Cell.

[18]  J. M. Adams,et al.  Expression of multiple homeobox genes within diverse mammalian haemopoietic lineages. , 1988, The EMBO journal.

[19]  Mary C. Rykowski,et al.  Human genome organization: Alu, LINES, and the molecular structure of metaphase chromosome bands , 1988, Cell.

[20]  W. Gehring,et al.  Homeo boxes in the study of development. , 1987, Science.

[21]  M. Levine,et al.  Homeo box gene expression in anterior and posterior regions of the Drosophila embryo. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[22]  E. Sánchez-Herrero,et al.  Genetic organization of Drosophila bithorax complex , 1985, Nature.

[23]  F. Mitelman,et al.  Catalog of Chromosome Aberrations in Cancer , 1996, British Journal of Cancer.

[24]  D. Ward,et al.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. , 1983, Virology.

[25]  E. Lewis.  A gene complex controlling segmentation in Drosophila , 1978, Nature.