Tracking X-ray microscopy for alveolar dynamics in live intact mice

Here we report a tracking X-ray microscopy (TrXM) as a novel methodology by using upper right lung apices alveoli in live intact mice. By enabling tracking of individual alveolar movements during respiration, TrXM identifies alveolar dynamics: individual alveoli in the upper lung apices show a small size increment as 4.9 ± 0.4% (mean ± s.e.m.) during respiration while their shapes look almost invariant. TrXM analysis in alveolar dynamics would be significant for better understanding of alveolar-based diseases, for instance, ventilator induced lung injury (VILI) in acute respiratory distress syndrome (ARDS).

[1]  G Margaritondo,et al.  Synchrotron microangiography with no contrast agent , 2004, Physics in medicine and biology.

[2]  Wah-Keat Lee,et al.  Advances in biological structure, function, and physiology using synchrotron X-ray imaging*. , 2008, Annual review of physiology.

[3]  R. Glenny,et al.  Stabilized Imaging of Immune Surveillance in the Mouse Lung , 2010, Nature Methods.

[4]  R. Robinson,et al.  Flow in a terminal alveolar sac model with expanding walls using computational fluid dynamics , 2010, Inhalation toxicology.

[5]  L. Gatto,et al.  Alveolar mechanics in the acutely injured lung: role of alveolar instability in the pathogenesis of ventilator-induced lung injury. , 2004, Respiratory care.

[6]  Edmund Koch,et al.  Virtual four-dimensional imaging of lung parenchyma by optical coherence tomography in mice. , 2010, Journal of biomedical optics.

[7]  Gary Nieman,et al.  Dynamic alveolar mechanics and ventilator-induced lung injury , 2005, Critical care medicine.

[8]  J. Haynes.  Lung Function: Physiology, Measurement, and Application in Medicine, 6th edition. , 2007 .

[9]  Edmund Koch,et al.  Alveolar dynamics in acute lung injury: Heterogeneous distension rather than cyclic opening and collapse* , 2009, Critical care medicine.

[10]  J. E. Cotes,et al.  Lung Function: Physiology, Measurement and Application in Medicine , 2006 .

[11]  F. S. Henry,et al.  Nanoparticle delivery in infant lungs , 2012, Proceedings of the National Academy of Sciences.

[12]  Christopher M Waters,et al.  What do we know about mechanical strain in lung alveoli? , 2011, American journal of physiology. Lung cellular and molecular physiology.

[13]  D. Schoenfeld,et al.  Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome. , 2000, The New England journal of medicine.

[14]  Kentaro Uesugi,et al.  Development of high-resolution 4D in vivo-CT for visualization of cardiac and respiratory deformations of small animals , 2008, Physics in medicine and biology.

[15]  Stuart B Hooper,et al.  Synchrotron-based dynamic computed tomography of tissue motion for regional lung function measurement , 2012, Journal of The Royal Society Interface.