An increase in ethylene sensitivity following pollination is the initial event triggering an increase in ethylene production and enhanced senescence of Phalaenopsis orchid flowers

Cut Phaluenopsis (Phaloenopsis hybrid, cv. Herbert Haper) flowers usually last about 2 weeks. Following pollination however, there is a rapid acceleration of the wilting process, which is completed within 2 days. The first event detected following pollination was an increase in ethylene sensitivity. This increased sensitivity began about 4 h after pollination and peaked 6 h later. A subsequent increase in ethylene production could only be detected 12 to 14 h after pollination. Treatment of the flowers with silver thiosulfate or 1-methylcyclopropene, both inhibitors of ethylene action, completely inhibited the pollination-induced increase in ethylene production and the enhanced senescence of the flowers. This indicates that the pollination-induced increase in ethylene production is a response to the existing ethylene. Treatment of flowers with calcium and its ionophore A23187, which increased ethylene sensitivity and protein phosphorylation, also promoted ethylene production and senescence of unpollinated flowers. EGTA, a calcium chelator, decreased the sensitivity of pollinated flowers to ethylene, and delayed and decreased the pollination-induced increase in ethylene production. We suggest that the pollination-induced increase in ethylene sensitivity is the initial pollination-induced event triggering the enhancement of ethylene production, which leads to enhanced senescence of Phalaenopsiss flowers