Encapsulating tantalum oxide into polypyrrole nanoparticles for X-ray CT/photoacoustic bimodal imaging-guided photothermal ablation of cancer.

A nanotheranostic agent has been readily fabricated by encapsulating tantalum oxide (TaOx) nanoparticles (NPs) into polypyrrole (PPy) NPs via a facile one-step chemical oxidation polymerization for bimodal imaging guided photothermal ablation of tumor. It was proved that the obtained composite nanoparticles (TaOx@PPy NPs) with an average diameter around 45 nm could operate as an efficient bimodal contrast agent to simultaneously enhance X-ray CT and photoacoustic (PA) imaging greatly in vivo. Systemically administered TaOx@PPy NPs could passively accumulate at the tumor site during the blood circulation, which was proved by both CT and PA imaging. In addition, the in vivo therapeutic examinations showed that TaOx@PPy NPs exhibited significant photothermal cytotoxicity under near infrared laser irradiation. The tumor growth inhibition was evaluated to be 66.5% for intravenously injection and 100% for intratumoral injection, respectively. This versatile agent can be developed as a smart and promising nanoplatform that integrates multiple capabilities for both accurate diagnosing and precise locating of cancerous tissue, as well as effective photoablation of tumor.

[1]  Taeghwan Hyeon,et al.  Multifunctional Fe3O4/TaO(x) core/shell nanoparticles for simultaneous magnetic resonance imaging and X-ray computed tomography. , 2012, Journal of the American Chemical Society.

[2]  Shiro Mori,et al.  Photothermal therapy of tumors in lymph nodes using gold nanorods and near-infrared laser light. , 2013, Journal of controlled release : official journal of the Controlled Release Society.

[3]  Warren C W Chan,et al.  Nanoparticle-mediated cellular response is size-dependent. , 2008, Nature nanotechnology.

[4]  Lihong V. Wang,et al.  Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model. , 2008, Journal of biomedical optics.

[5]  A. D. Watson,et al.  Metal-Based X-ray Contrast Media. , 1999, Chemical reviews.

[6]  Zhanwen Xing,et al.  Gold-nanoshelled microcapsules: a theranostic agent for ultrasound contrast imaging and photothermal therapy. , 2011, Angewandte Chemie.

[7]  M. Chu,et al.  A gold nanoshell with a silica inner shell synthesized using liposome templates for doxorubicin loading and near-infrared photothermal therapy , 2011, International journal of nanomedicine.

[8]  M. Adolphe,et al.  A non-isotopic, highly sensitive, fluorimetric, cell-cell adhesion microplate assay using calcein AM-labeled lymphocytes. , 1995, Journal of immunological methods.

[9]  D. Dong,et al.  Early detection of liver cancer based on bioluminescence tomography. , 2011, Applied optics.

[10]  Butrus T. Khuri-Yakub,et al.  Deep Tissue Photoacoustic Imaging Using a Miniaturized 2-D Capacitive Micromachined Ultrasonic Transducer Array , 2012, IEEE Transactions on Biomedical Engineering.

[11]  Kai Yang,et al.  Optimization of surface chemistry on single-walled carbon nanotubes for in vivo photothermal ablation of tumors. , 2011, Biomaterials.

[12]  Meifang Zhu,et al.  Hydrophilic Flower‐Like CuS Superstructures as an Efficient 980 nm Laser‐Driven Photothermal Agent for Ablation of Cancer Cells , 2011, Advanced materials.

[13]  Chad A. Mirkin,et al.  Gold nanoparticles for biology and medicine. , 2010, Angewandte Chemie.

[14]  R. Stafford,et al.  Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[15]  Petra Krystek,et al.  Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. , 2008, Biomaterials.

[16]  Matthew G. Panthani,et al.  Copper selenide nanocrystals for photothermal therapy. , 2011, Nano letters.

[17]  Liangping Zhou,et al.  Radiopaque fluorescence-transparent TaOx decorated upconversion nanophosphors for in vivo CT/MR/UCL trimodal imaging. , 2012, Biomaterials.

[18]  Younan Xia,et al.  Near-infrared gold nanocages as a new class of tracers for photoacoustic sentinel lymph node mapping on a rat model. , 2009, Nano letters.

[19]  V. Zharov,et al.  Golden carbon nanotubes as multimodal photoacoustic and photothermal high-contrast molecular agents. , 2009, Nature nanotechnology.

[20]  Manojit Pramanik,et al.  In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node , 2009, Physics in medicine and biology.

[21]  Zhifei Dai,et al.  Targeted delivery of CuS nanoparticles through ultrasound image-guided microbubble destruction for efficient photothermal therapy. , 2013, Nanoscale.

[22]  Kai Yang,et al.  Graphene in mice: ultrahigh in vivo tumor uptake and efficient photothermal therapy. , 2010, Nano letters.

[23]  Mauro Ferrari,et al.  Intravascular Delivery of Particulate Systems: Does Geometry Really Matter? , 2008, Pharmaceutical Research.

[24]  B. Vincent,et al.  Dispersions of electrically conducting polypyrrole particles in aqueous media , 1987 .

[25]  Samuel Woojoo Jun,et al.  Large-scale synthesis of bioinert tantalum oxide nanoparticles for X-ray computed tomography imaging and bimodal image-guided sentinel lymph node mapping. , 2011, Journal of the American Chemical Society.

[26]  Z. Dai,et al.  Polypyrrole Hollow Microspheres as Echogenic Photothermal Agent for Ultrasound Imaging Guided Tumor Ablation , 2013, Scientific Reports.

[27]  Younan Xia,et al.  Gold nanocages as photothermal transducers for cancer treatment. , 2010, Small.

[28]  Xiuli Yue,et al.  Prussian blue nanoparticles operate as a new generation of photothermal ablation agents for cancer therapy. , 2012, Chemical communications.

[29]  R. Drezek,et al.  Elimination of Metastatic Melanoma Using Gold Nanoshell-Enabled Photothermal Therapy and Adoptive T Cell Transfer , 2013, PloS one.

[30]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[31]  Timothy J Shaw,et al.  Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects. , 2009, Small.

[32]  M. Choti,et al.  Single-Center Phase II Trial of Transarterial Chemoembolization With Drug-Eluting Beads for Patients With Unresectable Hepatocellular Carcinoma: Initial Experience in the United States , 2009, Cancer journal.

[33]  F. Schick,et al.  Non-invasive assessment and quantification of liver steatosis by ultrasound, computed tomography and magnetic resonance. , 2009, Journal of hepatology.

[34]  Shuming Nie,et al.  Quantum dots and multifunctional nanoparticles: new contrast agents for tumor imaging. , 2006, Nanomedicine.

[35]  M. Farhadi,et al.  The effects of folate-conjugated gold nanorods in combination with plasmonic photothermal therapy on mouth epidermal carcinoma cells , 2014, Lasers in Medical Science.

[36]  Dong Liang,et al.  A chelator-free multifunctional [64Cu]CuS nanoparticle platform for simultaneous micro-PET/CT imaging and photothermal ablation therapy. , 2010, Journal of the American Chemical Society.

[37]  Changhui Li,et al.  Biocompatible polypyrrole nanoparticles as a novel organic photoacoustic contrast agent for deep tissue imaging. , 2013, Nanoscale.

[38]  Yushen Jin,et al.  Graphene oxide modified PLA microcapsules containing gold nanoparticles for ultrasonic/CT bimodal imaging guided photothermal tumor therapy. , 2013, Biomaterials.

[39]  D. Hanahan,et al.  The Hallmarks of Cancer , 2000, Cell.

[40]  H. Dai,et al.  Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. , 2011, Journal of the American Chemical Society.

[41]  Feng Gao,et al.  In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages. , 2010, ACS nano.

[42]  Hyeonseok Yoon,et al.  Kinetic study of the formation of polypyrrole nanoparticles in water-soluble polymer/metal cation systems: a light-scattering analysis. , 2010, Small.

[43]  Nastassja A. Lewinski,et al.  A new era for cancer treatment: gold-nanoparticle-mediated thermal therapies. , 2011, Small.

[44]  Fei Yang,et al.  Fast Katsevich Algorithm Based on GPU for Helical Cone-Beam Computed Tomography , 2010, IEEE Transactions on Information Technology in Biomedicine.

[45]  Qiushi Ren,et al.  Uniform Polypyrrole Nanoparticles with High Photothermal Conversion Efficiency for Photothermal Ablation of Cancer Cells , 2013, Advanced materials.

[46]  Xiaohua Huang,et al.  Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. , 2006, Journal of the American Chemical Society.

[47]  G. Jézéquel,et al.  Composition of natural oxide films on polycrystalline tantalum using XPS electron take‐off angle experiments , 1992 .

[48]  B. Liedberg,et al.  Electrically conducting composites of colloidal polypyrrole and methylcellulose , 1986 .

[49]  Hui Zhang,et al.  Immuno gold nanocages with tailored optical properties for targeted photothermal destruction of cancer cells. , 2007, Nano letters.

[50]  Lihong V. Wang,et al.  Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs , 2012, Science.

[51]  H. S. Fogler,et al.  Controlled Formation of Silica Particles from Tetraethyl Orthosilicate in Nonionic Water-in-Oil Microemulsions , 1997 .