Identification of synergistic interactions among microorganisms in biofilms by digital image analysis.

Digital image analysis showed that reductions in biofilm plating efficiency were due to the loss of protection provided by two benzoate-degrading strains of Pseudomonas fluorescens. This loss in protection was due to the spatial separation of the protective organisms from benzoate-sensitive organisms during the dilution process. Communities were cultivated in flow cells irrigated with trypticase soy broth. When the effluent from these flow cells was plated on 0.15% benzoic acid, satellite colonies formed only in the vicinity of primary colonies. A digital image analysis procedure was developed to measure the size and spatial distribution of these satellites as a function of distance from the primary colony. The size of satellites served as a measure of growth, and the number per unit area served as a measure of survival. At the three dilutions tested, the size and concentration of satellite colonies varied inversely with distance from the primary colonies. When these measurements were plotted, the slopes were used to quantify the effect of bacterial association on the growth and survivability of the satellites. In the absence of the primary colonies, satellites grew in axenic culture only at low benzoate concentrations. Thus benzoate-degrading organisms are capable of creating a protective microenvironment for other members of biofilm communities.

[1]  B. Efiuvwevwere,et al.  THE EFFECTS OF CHEMICAL PRESERVATIVES AND PASTEURIZATION ON THE MICROBIAL SPOILAGE AND SHELF‐LIFE OF KUNUN‐ZAKI , 1997 .

[2]  G. Barker,et al.  Spatial interactions between subsurface bacterial colonies in a model system: a territory model describing the inhibition of Listeria monocytogenes by a nisin-producing lactic acid bacterium. , 1997, Microbiology.

[3]  S. Karthikeyan,et al.  Germ Theory Vs. Community Theory in Understanding and Controlling the Proliferation of Biofilms , 1997, Advances in dental research.

[4]  J. Lawrence,et al.  Do Bacterial Communities Transcend Darwinism , 1997 .

[5]  Cal A. de,et al.  Are bacterial biofilms constrained to Darwin's concept of evolution through natural selection? , 1996 .

[6]  I. Gould,et al.  Phenoxymethyl penicillin versus co-amoxiclav in the treatment of acute streptococcal pharyngitis, and the role of beta-lactamase activity in saliva. , 1996, The Journal of antimicrobial chemotherapy.

[7]  J. Costerton,et al.  Are bacterial biofilms constrained to Darwin's concept of evolution through natural selection? , 1996, Microbiologia.

[8]  C. V. van Ginkel Complete degradation of xenobiotic surfactants by consortia of aerobic microorganisms. , 1996, Biodegradation.

[9]  J. Wimpenny,et al.  Submerged bacterial colonies within food and model systems: their growth, distribution and interactions. , 1995, International journal of food microbiology.

[10]  L. Restaino,et al.  Antimicrobial Effectiveness of Potassium Sorbate and Sodium Benzoate against Zygosaccharomyces bailii in a Salsa Mayonnaise. , 1995, Journal of food protection.

[11]  C. Genigeorgis,et al.  Potential growth and control of Escherichia coli O157:H7 in soft hispanic type cheese. , 1995, International journal of food microbiology.

[12]  A. Bouvet Human endocarditis due to nutritionally variant streptococci: streptococcus adjacens and Streptococcus defectivus. , 1995, European heart journal.

[13]  E. Østergaard Evaluation of the antimicrobial effects of sodium benzoate and dichlorobenzyl alcohol against dental plaque microorganisms. An in vitro study , 1994 .

[14]  J. Lawrence,et al.  The role of interactions, sessile growth and nutrient amendments on the degradative efficiency of a microbial consortium. , 1994, Canadian journal of microbiology.

[15]  E. Ostergaard Evaluation of the antimicrobial effects of sodium benzoate and dichlorobenzyl alcohol against dental plaque microorganisms. An in vitro study. , 1994, Acta odontologica Scandinavica.

[16]  R. Osawa,et al.  Characterization of Gram-negative Anaerobic Strains, Isolated from Koala Feces, which Exhibit Satellite Growth and Pleomorphism , 1992 .

[17]  S. Dagley,et al.  CHAPTER 15 – Biochemistry of Aromatic Hydrocarbon Degradation in Pseudomonads , 1986 .

[18]  H. Benson Microbiological Applications: A Laboratory Manual in General Microbiology , 1985 .

[19]  W. Traub Simple screening method for gram-positive bacterial beta-lactam antibiotic tolerance on routine laboratory Bauer-Kirby antibiogram plates. , 1982, Chemotherapy.

[20]  M. Mccarty,et al.  Nutritionally variant streptococci from patients with endocarditis: growth parameters in a semisynthetic medium and demonstration of a chromophore , 1981, Journal of bacteriology.

[21]  R. E. Buchanan,et al.  Bergey's Manual of Determinative Bacteriology. , 1975 .

[22]  R. H. George The isolation of symbiotic streptococci. , 1974, Journal of medical microbiology.

[23]  L. S. Mcclung Laboratory Manual for General Microbiology J. V. Beck D. H. Larson D. M. Donaldson R. D. Sagers , 1961 .

[24]  W. Hirsch,et al.  Spontaneous Development of L Forms of Streptococci requiring Secretions of Other Bacteria or Sulphydryl Compounds for Normal Growth , 1961, Nature.

[25]  S. T. Cowan Bergey's Manual of Determinative Bacteriology , 1948, Nature.