Cell Biology International

Long noncoding RNA (lncRNA) small nucleolar RNA host gene 16 (SNHG16) has been linked to multiple cancers including breast, ovarian, bladder, and colorectal cancer. However, the role of SNHG16 in cervical cancer is unclear. Here, quantitative analysis of SNHG16 and PARP9 expression levels in cervical cancer tissues and cell lines indicated that both SNHG16 and PARP9 were highly expressed compared with controls. Using the dual‐luciferase reporter gene assay, RNA immunoprecipitation, chromatin immunoprecipitation, we were able to determine that SNHG16 recruited SPI1 protein to promote transcription of PARP9 to upregulate its transcription in cervical cancer cells. After ectopic expression and knockdown experiments were conducted, it was observed that silencing SNHG16 inhibited PARP9 expression, proliferation, and invasion of cervical cancer cells, which was rescued by co‐transfection of SNHG16 silencing and PARP9 overexpression. Moreover, in vivo experimental results showed that silencing SNHG16 reduced the expression of PARP9 and suppressed tumor growth. These data indicate that SNHG16 recruits SPI1 to upregulate PARP9, which promotes the tumorigenicity of cervical cancer cells. The regulation of their expression might provide a new direction for treating cervical cancer.

[1]  S. Ghorbian,et al.  Long non-coding RNAs and cervical cancer. , 2019, Experimental and molecular pathology.

[2]  Y. Ye,et al.  LncRNA SNHG16 predicts poor prognosis in ESCC and promotes cell proliferation and invasion by regulating Wnt/β-catenin signaling pathway. , 2018, European review for medical and pharmacological sciences.

[3]  S. Gu,et al.  Transcription factor PU.1 is involved in the progression of glioma , 2018, Oncology letters.

[4]  Jun Liu,et al.  MiR-216b inhibits cell proliferation by targeting FOXM1 in cervical cancer cells and is associated with better prognosis , 2017, BMC Cancer.

[5]  Man-man Su,et al.  Long non-coding RNAs on the stage of cervical cancer (Review). , 2017, Oncology reports.

[6]  Q. Shang,et al.  Effects of DCK knockdown on proliferation, apoptosis and tumorigenicity in vivo of cervical cancer HeLa cells , 2017, Cancer Gene Therapy.

[7]  J. Vider,et al.  MicroRNA‐186‐5p overexpression modulates colon cancer growth by repressing the expression of the FAM134B tumour inhibitor , 2017, Experimental cell research.

[8]  H. Kitchener,et al.  Cervical cancer: A global health crisis , 2017, Cancer.

[9]  Yan Wang,et al.  Long Noncoding RNA ANRIL Promotes Cervical Cancer Development by Acting as a Sponge of miR-186. , 2017, Oncology research.

[10]  Steven J. M. Jones,et al.  Integrated genomic and molecular characterization of cervical cancer , 2017, Nature.

[11]  Jakob Skou Pedersen,et al.  SNHG16 is regulated by the Wnt pathway in colorectal cancer and affects genes involved in lipid metabolism , 2016, Molecular oncology.

[12]  Kathryn E. Hentges,et al.  Transcriptional regulation of the proto‐oncogene Zfp521 by SPI1 (PU.1) and HOXC13 , 2016, Genesis.

[13]  C. Peterson,et al.  A somatic mutation of GFI1B identified in leukemia alters cell fate via a SPI1 (PU.1) centered genetic regulatory network. , 2016, Developmental biology.

[14]  M. Hottiger.  Poly(ADP-ribose) polymerase inhibitor therapeutic effect: are we just scratching the surface? , 2015, Expert opinion on therapeutic targets.

[15]  Jun S. Liu,et al.  Inference of transcriptional regulation in cancers , 2015, Proceedings of the National Academy of Sciences.

[16]  Sejal Vyas,et al.  New PARP targets for cancer therapy , 2014, Nature Reviews Cancer.

[17]  Sejal Vyas,et al.  A systematic analysis of the PARP protein family identifies new functions critical for cell physiology , 2013, Nature Communications.

[18]  A. Seth,et al.  ETS transcription factors and their emerging roles in human cancer. , 2005, European journal of cancer.

[19]  A. Albor,et al.  Regulation of the human poly(ADP-ribose) polymerase promoter by the ETS transcription factor , 1999, Oncogene.

[20]  A. Boyles,et al.  National Institute of Environmental Health Sciences , 2008 .

[21]  K. Su,et al.  A novel positive feedback regulation between long noncoding RNA UICC and IL-6/STAT3 signaling promotes cervical cancer progression. , 2018, American journal of cancer research.

[22]  H. Hua,et al.  PARP 9 is overexpressed in human breast cancer and promotes cancer cell migration , 2018 .

[23]  Hong Zhu,et al.  SNHG16/miR-216-5p/ZEB1 signal pathway contributes to the tumorigenesis of cervical cancer cells. , 2018, Archives of biochemistry and biophysics.

[24]  Yi Feng,et al.  Long Non-Coding RNAs , 2016, Methods in Molecular Biology.