Comparison of big bluestem with other native grasses: chemical composition and biofuel yield.

Multiple entry selections of big bluestems and three native C4 grass species, including switchgrass, miscanthus, and Conservation Reserve Program (CRP) mixture grass, were evaluated for their chemical composition and ethanol yields via diluted sulfuric acid pretreatment following simultaneous saccharification and fermentation (SSF). Big bluestem and switchgrass had a similar glucan content that was significantly higher than miscanthus and CRP grass. Big bluestem had the highest average mass recovery (55.56%) after acid pretreatment, and miscanthus had the lowest mass recovery (46.3%). A positive correlation was observed between glucan recovery and mass recovery. No significant difference in average efficiency of SSF was observed among four native grasses, but ethanol yields from big bluestem entries, which averaged 26.2%, were consistently greater than the other three grasses. The highest ethanol yield among the 10 entries was from big bluestem cultivar KAW (27.7%). Approximately 0.26 kg ethanol with 9.4 g/L concentration can be produced from 1 kg of big bluestem biomass under current processing conditions. A negative relationship exists between lignin content and the efficiency of SSF with R = −0.80, and a positive relationship exists between ethanol yield and glucan content with R = 0.71.

[1]  P. Reich,et al.  Diversity and Productivity in a Long-Term Grassland Experiment , 2001, Science.

[2]  K. Moore,et al.  Switchgrass as a Bioenergy Crop: Biomass Production and Soil Carbon Sequestration in Response to Nitrogen Fertilization [abstract] , 2009 .

[3]  Zsófia Kádár,et al.  Sweet Sorghum as Feedstock for Ethanol Production: Enzymatic Hydrolysis of Steam-Pretreated Bagasse , 2009, Applied biochemistry and biotechnology.

[4]  Mohammad J. Taherzadeh,et al.  Alkaline pretreatment of spruce and birch to improve bioethanol and biogas production , 2010, BioResources.

[5]  Markus Pauly,et al.  Cell-wall carbohydrates and their modification as a resource for biofuels. , 2008, The Plant journal : for cell and molecular biology.

[6]  B. Saha,et al.  Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol , 2005 .

[7]  J. Scurlock,et al.  Miscanthus : European experience with a novel energy crop , 2000 .

[8]  Ignacio Ballesteros,et al.  Ethanol production from steam-explosion pretreated wheat straw , 2006, Applied biochemistry and biotechnology.

[9]  Stephen P. Long,et al.  Meeting US biofuel goals with less land: the potential of Miscanthus , 2008 .

[10]  Anthony Dufour,et al.  Miscanthus: a fast‐growing crop for biofuels and chemicals production , 2012 .

[11]  R. Gonzalez,et al.  Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. , 2007, Current opinion in biotechnology.

[12]  K. Linnebur.  Analysis of torrefaction of big bluestem and mixed grass from the Conservation Reserve Program , 2013 .

[13]  D. Tilman,et al.  Carbon-Negative Biofuels from Low-Input High-Diversity Grassland Biomass , 2006, Science.

[14]  Shing I. Chang,et al.  Glucan Yield from Enzymatic Hydrolysis of Big Bluestem as Affected by Ecotype and Planting Location Along the Precipitation Gradient of the Great Plains , 2014, BioEnergy Research.

[15]  J. Fike,et al.  The Biology and Agronomy of Switchgrass for Biofuels , 2005 .

[16]  Zhijian Pei,et al.  Thermal properties of big bluestem as affected by ecotype and planting location along the precipitation gradient of the Great Plains , 2014 .

[17]  Michael D. Montross,et al.  Regional examination shows potential for native feedstock options for cellulosic biofuel production , 2009 .

[18]  M. Galbe,et al.  Fuel ethanol production from steam-pretreated corn stover using SSF at higher dry matter content , 2006 .

[19]  Teerapatr Srinorakutara,et al.  Evaluation of dilute-acid pretreated bagasse, corn cob and rice straw for ethanol fermentation bySaccharomyces cerevisiae , 2008, Annals of Microbiology.

[20]  K. Réczey,et al.  High solid simultaneous saccharification and fermentation of wet oxidized corn stover to ethanol. , 2004, Biotechnology and bioengineering.

[21]  K. Muthukumarappan,et al.  Effect of extruder parameters and moisture content of corn stover and big bluestem on sugar recovery from enzymatic hydrolysis. , 2010 .

[22]  D. Naugle,et al.  EFFECTS OF WIND TURBINES ON UPLAND NESTING BIRDS IN CONSERVATION RESERVE PROGRAM GRASSLANDS , 1999 .

[23]  Tao Sang,et al.  Pretreatment on Miscanthus lutarioriparious by liquid hot water for efficient ethanol production , 2013, Biotechnology for Biofuels.

[24]  Uffe Jørgensen,et al.  Genotypic variation in dry matter accumulation and content of N, K and Cl in Miscanthus in Denmark. , 1997 .

[25]  Clarence Lehman,et al.  Bioenergy and Wildlife: Threats and Opportunities for Grassland Conservation , 2009 .

[26]  M. Penner,et al.  Enzymatic saccharification and fermentation of xylose-optimized dilute acid-treated lignocellulosics , 2005, Applied biochemistry and biotechnology.

[27]  Lynn L Wright,et al.  Historical Perspective on How and Why Switchgrass was Selected as a "Model" High-Potential Energy Crop , 2007 .

[28]  D. K. Lee,et al.  Switchgrass, Big Bluestem, and Indiangrass Monocultures and Their Two- and Three-Way Mixtures for Bioenergy in the Northern Great Plains , 2012, BioEnergy Research.

[29]  John R. Matchett,et al.  FIRE AND GRAZING REGULATE BELOWGROUND PROCESSES IN TALLGRASS PRAIRIE , 2001 .

[30]  J. H. Cushman,et al.  Herbaceous energy crops: planning for a renewed commitment. [Hay, silage, rapeseed, sugar and starch crops, hydrocarbon crops] , 1984 .

[31]  Donghai Wang,et al.  Hydrothermal conversion of big bluestem for bio-oil production: the effect of ecotype and planting location. , 2012, Bioresource technology.

[32]  Andrew D. Jones,et al.  Supporting Online Material for: Ethanol Can Contribute To Energy and Environmental Goals , 2006 .

[33]  David D. Baltensperger,et al.  Leaf and Stem Yields and Forage Quality of Three N‐fertilized Warm‐Season Grasses1 , 1979 .

[34]  Yuan Liu,et al.  Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of Miscanthus sinensis to Monosaccharides , 2008, Bioscience, biotechnology, and biochemistry.

[35]  Jianxin Jiang,et al.  Effect of lignin content on enzymatic hydrolysis of furfural residues. , 2011 .

[36]  I. Madakadze,et al.  Evaluation of selected warm-season grasses for biomass production in areas with a short growing season , 1998 .

[37]  R. Mueller,et al.  The 2009 Cropland Data Layer. , 2010 .

[38]  P. Weimer,et al.  Fermentability of eastern gamagrass, big bluestem and sand bluestem grown across a wide variety of environments. , 2007, Bioresource technology.

[39]  Kenneth J. Moore,et al.  Composition of native warm-season grasses for bioenergy production in response to nitrogen fertilization rate and harvest date. , 2011 .

[40]  M. Cotta,et al.  Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents , 2009, BioEnergy Research.

[41]  Ethanol production from disposable aspen chopsticks using delignification pretreatments , 2011 .

[42]  Young-Lok Cha,et al.  Optimization of alkali-extrusion pretreatment with twin-screw for bioethanol production from Miscanthus , 2013 .

[43]  Ke Zhang,et al.  The effect of ecotype and planting location on properties and biofuels yield of big bluestem , 2014 .

[44]  Bryce J. Stokes,et al.  Biomass as Feedstock for A Bioenergy and Bioproducts Industry: The Technical Feasibility of a Billion-Ton Annual Supply , 2005 .

[45]  M. Holtzapple,et al.  Lime pretreatment and enzymatic hydrolysis of corn stover. , 2005, Bioresource technology.

[46]  Zhijian Pei,et al.  Chemical and elemental composition of big bluestem as affected by ecotype and planting location along the precipitation gradient of the Great Plains , 2012 .

[47]  B. Venuto,et al.  Biomass Feedstock Harvest from Conservation Reserve Program Land in Northwestern Oklahoma , 2010, Crop Science.