The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex

Depletion of the Nup188–Nup93 nuclear pore complex results in enlarged nuclei due to unrestricted protein import.

[1]  M. D'Angelo,et al.  Structure, dynamics and function of nuclear pore complexes. , 2008, Trends in cell biology.

[2]  H. Worman,et al.  Signals and structural features involved in integral membrane protein targeting to the inner nuclear membrane , 1995, The Journal of cell biology.

[3]  I. Mattaj Sorting out the nuclear envelope from the endoplasmic reticulum , 2004, Nature Reviews Molecular Cell Biology.

[4]  T. Giddings,et al.  Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction. , 2000, Journal of structural biology.

[5]  M. Hochstrasser,et al.  Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase , 2006, Nature.

[6]  D. Goldfarb,et al.  The nucleoporins Nup170p and Nup157p are essential for nuclear pore complex assembly , 2009, The Journal of cell biology.

[7]  K Ribbeck,et al.  Kinetic analysis of translocation through nuclear pore complexes , 2001, The EMBO journal.

[8]  M. Hetzer,et al.  MEL‐28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly , 2007, EMBO reports.

[9]  B. Burke,et al.  Internuclear exchange of an inner nuclear membrane protein (p55) in heterokaryons: in vivo evidence for the interaction of p55 with the nuclear lamina , 1990, The Journal of cell biology.

[10]  W. Fischer,et al.  Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export , 2001, The Journal of cell biology.

[11]  A. E. Dahlberg,et al.  Enhancement of translation by the epsilon element is independent of the sequence of the 460 region of 16S rRNA. , 2001, Nucleic acids research.

[12]  Karsten Weis,et al.  Regulating Access to the Genome Nucleocytoplasmic Transport throughout the Cell Cycle , 2003, Cell.

[13]  R. Wepf,et al.  Nic 96 p Is Required for Nuclear Pore Formation and Functionally Interacts with a Novel Nucleoporin , Nup 188 p , 2002 .

[14]  Friedrich Förster,et al.  Snapshots of nuclear pore complexes in action captured by cryo-electron tomography , 2007, Nature.

[15]  S. Briggs,et al.  ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division , 2006, Proceedings of the National Academy of Sciences.

[16]  S. Boué,et al.  Direct membrane protein–DNA interactions required early in nuclear envelope assembly , 2006, The Journal of cell biology.

[17]  V. Shahin,et al.  A Pathway Separate from the Central Channel through the Nuclear Pore Complex for Inorganic Ions and Small Macromolecules* , 2007, Journal of Biological Chemistry.

[18]  D. Forbes,et al.  Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation , 1995, The Journal of cell biology.

[19]  E. C. Schirmer,et al.  Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore , 2004, The Journal of cell biology.

[20]  Elena Conti,et al.  Structural biology of nucleocytoplasmic transport. , 2007, Annual review of biochemistry.

[21]  J. Kartenbeck,et al.  NSF- and SNARE-mediated membrane fusion is required for nuclear envelope formation and completion of nuclear pore complex assembly in Xenopus laevis egg extracts , 2007, Journal of Cell Science.

[22]  G. Blobel,et al.  Highway to the inner nuclear membrane: rules for the road , 2007, Nature Reviews Molecular Cell Biology.

[23]  M. Hetzer,et al.  Pushing the envelope: structure, function, and dynamics of the nuclear periphery. , 2005, Annual review of cell and developmental biology.

[24]  M. Rout,et al.  The Nuclear Pore Complex as a Transport Machine* , 2001, The Journal of Biological Chemistry.

[25]  R. Wozniak,et al.  Nup53 is required for nuclear envelope and nuclear pore complex assembly. , 2008, Molecular biology of the cell.

[26]  R. Wepf,et al.  Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p , 1996, The Journal of cell biology.

[27]  J. Blow,et al.  Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly. , 1990, Journal of cell science.

[28]  Minoru Yoshida,et al.  CRM1 Is an Export Receptor for Leucine-Rich Nuclear Export Signals , 1997, Cell.

[29]  B. Chait,et al.  Determining the architectures of macromolecular assemblies , 2007, Nature.

[30]  D. Goldfarb,et al.  Yeast Nucleoporins Involved in Passive Nuclear Envelope Permeability , 2000, The Journal of cell biology.

[31]  G. Blobel,et al.  The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex , 1996, The Journal of cell biology.

[32]  B. Klupp,et al.  Herpesvirus assembly: an update. , 2009, Virus research.

[33]  M. Hetzer,et al.  The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly , 2003, Cell.

[34]  G. Blobel,et al.  Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[35]  E. Zimmerman,et al.  Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores. , 2003, Molecular cell.

[36]  Wolfram Antonin,et al.  The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation. , 2005, Molecular cell.

[37]  E. Hurt,et al.  Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. , 1997, Annual review of genetics.

[38]  M. Mann,et al.  Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. , 1997, Molecular biology of the cell.

[39]  Steffen Frey,et al.  Characterisation of the passive permeability barrier of nuclear pore complexes , 2009, The EMBO journal.

[40]  Ario de Marco,et al.  Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates , 2005, The EMBO journal.

[41]  Wolfram Antonin,et al.  The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells. , 2006, Molecular cell.

[42]  B. Chait,et al.  The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism , 2000 .

[43]  V. Brumfeld,et al.  Passive and Facilitated Transport in Nuclear Pore Complexes Is Largely Uncoupled* , 2006, Journal of Biological Chemistry.

[44]  R. Milligan,et al.  Nuclear pore complexes exceeding eightfold rotational symmetry. , 2003, Journal of structural biology.

[45]  Vincent Galy,et al.  Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo. , 2003, Molecular biology of the cell.

[46]  Eric D. Spear,et al.  Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats , 2008, Science.

[47]  E. Hallberg,et al.  Phosphomimetic mutation of the mitotically phosphorylated serine 1880 compromises the interaction of the transmembrane nucleoporin gp210 with the nuclear pore complex. , 2007, Experimental cell research.

[48]  J. Ellenberg,et al.  Nuclear pore complex assembly through the cell cycle: Regulation and membrane organization , 2008, FEBS letters.

[49]  M. Hetzer,et al.  Nuclear Pores Form de Novo from Both Sides of the Nuclear Envelope , 2006, Science.

[50]  J. Olivo,et al.  Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis , 1999, Current Biology.

[51]  G. Blobel,et al.  Karyopherin-mediated import of integral inner nuclear membrane proteins , 2006, Nature.

[52]  Roland Riek,et al.  NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression , 2005, Science.

[53]  E. C. Schirmer,et al.  Inner nuclear membrane protein transport is mediated by multiple mechanisms. , 2008, Biochemical Society transactions.