Distribution of Porphyromonas gingivalis fimA genotypes in isolates from subgingival plaque and blood sample during bacteremia

Introduccion. Porphyromonas gingivalis es el principal agente etiologico de la periodontitis. El gen fimA ha sido relacionado con la virulencia del microorganismo, lo cual sugiere la participacion de dicho gen en la capacidad del microorganismo para alcanzar el torrente sanguineo. Objetivo. Estudiar la distribucion de los tipos de fimA de P. gingivalis en muestras de placa subgingival y de sangre obtenidas durante bacteriemias despues de raspaje y alisado radicular. Materiales y metodos. Se practico un alisado radicular a 15 pacientes con periodontitis. Se obtuvieron aislamientos clinicos de P. gingivalis de la placa subgingival y durante la bacteriemia inducida por el procedimiento. Para la genotipificacion se utilizo la tecnica de reaccion en cadena de la polimerasa (PCR) especifica para fimA. En los aislamientos no clasificables por PCR se realizo secuenciacion del gen fimA. Resultados. Seis pacientes fueron positivos para bacteriemia por P. gingivalis. La distribucion de fimA evaluada en 30 aislamientos de placa subgingival y de sangre mostro una mayor frecuencia del fimA tipo II de P. gingivalis. En los aislamientos de placa subgingival, la deteccion de fimA tipo II fue seguida por Ib, III y IV; sin embargo, en los aislamientos de sangre el tipo II fue seguido por los tipos IV, Ib y III. Conclusion. En los aislamientos de sangre y de placa subgingival de pacientes con periodontitis el fimA mas frecuente fue el tipo II; no fue posible correlacionar el tipo de fimA con la bacteriemia inducida por el alisado radicular. Los resultados de la secuenciacion del gen fimA no concuerdan con los obtenidos por PCR.

[1]  M. Bonnaure-Mallet,et al.  Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis. , 2008, Journal of clinical periodontology.

[2]  M. Hattori,et al.  Determination of the Genome Sequence of Porphyromonas gingivalis Strain ATCC 33277 and Genomic Comparison with Strain W83 Revealed Extensive Genome Rearrangements in P. gingivalis , 2008, DNA research : an international journal for rapid publication of reports on genes and genomes.

[3]  D. Caugant,et al.  fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis , 2007, Journal of Clinical Microbiology.

[4]  M. F. Torres,et al.  Periodontopathic microorganisms in peripheric blood after scaling and root planing. , 2007, Journal of clinical periodontology.

[5]  G. Hajishengallis,et al.  Differential Activation of Human Gingival Epithelial Cells and Monocytes by Porphyromonas gingivalis Fimbriae , 2006, Infection and Immunity.

[6]  M. Kilian,et al.  Incidence of bacteremia after chewing, tooth brushing and scaling in individuals with periodontal inflammation. , 2006, Journal of clinical periodontology.

[7]  J. Beck,et al.  Progressive Periodontal Disease and Risk of Very Preterm Delivery , 2006, Obstetrics and gynecology.

[8]  Brian Burt,et al.  Position paper: epidemiology of periodontal diseases. , 2005, Journal of periodontology.

[9]  D. Kinane,et al.  Bacteraemia following periodontal procedures , 2005, Journal of clinical periodontology.

[10]  F. Chandad,et al.  Modulation of cytokine production by Porphyromonas gingivalis in a macrophage and epithelial cell co-culture model. , 2005, Microbes and infection.

[11]  R. Ocaña-Riola,et al.  Periodontitis as risk factor for acute myocardial infarction. A case control study of Spanish adults. , 2005, Journal of periodontal research.

[12]  M. Mayer,et al.  Distribution of fimA genotypes of Porphyromonas gingivalis in subjects with various periodontal conditions. , 2004, Oral microbiology and immunology.

[13]  K. Prior,et al.  Prevalence of Porphyromonas gingivalis fimA genotypes in Caucasians. , 2003, European journal of oral sciences.

[14]  Ian T. Paulsen,et al.  Complete Genome Sequence of the Oral Pathogenic Bacterium Porphyromonas gingivalis Strain W83 , 2003, Journal of bacteriology.

[15]  H. Karch,et al.  Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination. , 2003, Microbiology.

[16]  S. Hamada,et al.  Identification of a new variant of fimA gene of Porphyromonas gingivalis and its distribution in adults and disabled populations with periodontitis. , 2002, Journal of periodontal research.

[17]  R. Genco,et al.  Interactions of oral pathogens with toll-like receptors: possible role in atherosclerosis. , 2002, Annals of periodontology.

[18]  J. Einax,et al.  Interaction of Porphyromonas gingivalis with KB cells: comparison of different clinical isolates. , 2002, Oral microbiology and immunology.

[19]  F. C. Gibson,et al.  Fimbria-Dependent Activation of Cell Adhesion Molecule Expression in Porphyromonas gingivalis-Infected Endothelial Cells , 2002, Infection and Immunity.

[20]  M. D'Andrea,et al.  Arginine-Specific Protease fromPorphyromonas gingivalis Activates Protease-Activated Receptors on Human Oral Epithelial Cells and Induces Interleukin-6 Secretion , 2001, Infection and Immunity.

[21]  S. Hamada,et al.  Prevalence of Specific Genotypes of Porphyromonas gingivalis fimA and Periodontal Health Status , 2000, Journal of dental research.

[22]  S. Hamada,et al.  Distribution and Molecular Characterization ofPorphyromonas gingivalis Carrying a New Type offimA Gene , 2000, Journal of Clinical Microbiology.

[23]  G. Armitage,et al.  Development of a classification system for periodontal diseases and conditions. , 1999, Annals of periodontology.

[24]  R. Lamont,et al.  Promoter Architecture of the Porphyromonas gingivalis Fimbrillin Gene , 1999, Infection and Immunity.

[25]  S. Hamada,et al.  Distribution of Porphyromonas gingivalisStrains with fimA Genotypes in Periodontitis Patients , 1999, Journal of Clinical Microbiology.

[26]  S. Hamada,et al.  The importance of fimbriae in the virulence and ecology of some oral bacteria. , 1998, Oral microbiology and immunology.

[27]  J. Slots,et al.  Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. , 1996, Oral microbiology and immunology.

[28]  S. Hamada,et al.  Molecular cloning and sequencing of the fimbrilin gene of Porphyromonas gingivalis strains and characterization of recombinant proteins. , 1993, Biochemical and biophysical research communications.

[29]  J. Mylotte,et al.  Colonization of dental plaque by respiratory pathogens in medical intensive care patients , 1992, Critical care medicine.

[30]  R. Genco,et al.  Porphyromonas (Bacteroides) gingivalis fimbrillin: size, amino-terminal sequence, and antigenic heterogeneity , 1991, Infection and immunity.

[31]  P. Söder,et al.  Detection and quantitation by lysis-filtration of bacteremia after different oral surgical procedures , 1990, Journal of clinical microbiology.

[32]  S. Hamada,et al.  Distribution of genotypes of Porphyromonas gingivalis in type 2 diabetic patients with periodontitis in Mexico. , 2007, Journal of clinical periodontology.