The role of elastin in the mechanical properties of skin.

The elastin fibers of rat skin samples were degraded by the use of a purified preparation of elastase to which soybean inhibitor was added, preventing the collagenolytic activity of the elastase on collagen. Control experiments ascertained degradation of elastin and no effect on collagen. The mechanical properties of the skin samples were studied before and after the enzymatic treatment and differences ascribed to the degraded elastin fibers. Elastin plays a role in the mechanical behaviour of rat skin at small stress values and small deformations. Especially, the elastin fibers are responsible for the recoiling mechanism after a stress or deformation has been applied.

[1]  K. Langer,et al.  On the anatomy and physiology of the skin: II. Skin Tension (With 1 Figure) , 1978 .

[2]  Pieter Wijn,et al.  The alinear viscoelastic properties of human skin in vivo for small deformations , 1980 .

[3]  B. Sykes,et al.  Molecular weight heterogeneity of the α-chain sub-units of collagen , 1971 .

[4]  E. Dempsey,et al.  The structure and chemical characterization of elastic fibers as reveled by elastase and by electron microscopy , 1952, The Anatomical record.

[5]  M. Sato.  [Mechanical properties of living tissues]. , 1986, Iyo denshi to seitai kogaku. Japanese journal of medical electronics and biological engineering.

[6]  Missirlis Yf.  Use of enzymolysis techniques in studying the mechanical properties of connective tissue components. , 1977 .

[7]  H. Fullmer.  A COMPARATIVE HISTOCHEMICAL STUDY OF ELASTIC, PRE-ELASTIC AND OXYTALAN CONNECTIVE TISSUE FIBERS , 1960, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[8]  J. G. Leslie,et al.  Elastin structure, biosynthesis, and relation to disease states. , 1981, The New England journal of medicine.

[9]  J. Clark,et al.  Elastic properties of single elastic fibers. , 1962, Journal of applied physiology.

[10]  J. Manschot,et al.  The mechanical properties of human skin in vivo , 1985 .

[11]  R J Minns,et al.  The role of the fibrous components and ground substance in the mechanical properties of biological tissues: a preliminary investigation. , 1973, Journal of biomechanics.

[12]  Joon B. Park.  Structure-Property Relationships of Biological Materials , 1979 .

[13]  H. Oxlund.  Changes in connective tissues during corticotrophin and corticosteroid treatment. Biomechanical and biochemical studies on muscle tendon, skin and aorta in experimental animals. , 1984, Danish medical bulletin.

[14]  H. Oxlund,et al.  The roles of hyaluronic acid, collagen and elastin in the mechanical properties of connective tissues. , 1980, Journal of anatomy.

[15]  M. Spina,et al.  Application of affinity chromatography to the purification of collagenase for the isolation of insoluble elastin. , 1975, Biochimica et biophysica acta.