Molecular marker analysis of kernel size and shape in bread wheat

The economic value of wheat grain is determined by the kernel morphology which is an important parameter for manufacturing different food products requiring specific grain characteristics. Although kernel size and shape have emerged as important breeding objectives, not much information is available about the number or location of associated gene(s)/quantitative trait loci. In the present study, a recombinant inbred line population of 106 plants (F 7 ) was phenotyped for four traits, namely kernel length. width, weight and factor form density (FFD) and genotyped with different polymerase chain reaction-based markers. Transgressive segregants were observed for all the traits and genetic correlation studies showed positive correlations between the majority of the traits. The number of markers associated with each trait ranged from two to nine and the phenotypic contribution by an individual marker ranged from 3.3 to 16.6%. Many of the markers showed linkage to more than one trait. Strategies for improving the wheat grain quality traits and the utility of such markers in marker-assisted selection (MAS) efforts are discussed.

[1]  J. Anderson,et al.  Quantitative Trait Loci Associated with Kernel Traits in a Soft × Hard Wheat Cross , 1999 .

[2]  A. Giura,et al.  Chromosomal location of genes controlling grain size in a large grained selection of wheat (Triticum aestivum L.) , 2004, Euphytica.

[3]  M. Daly,et al.  MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. , 1987, Genomics.

[4]  W. Powell,et al.  The use of AFLPs to examine genetic relatedness in barley , 1997, Molecular Breeding.

[5]  T. G. Chastain,et al.  Stand Establishment Response of Soft White Winter Wheat to Seedbed Residue and Seed Size , 1995 .

[6]  P. Langridge,et al.  Mapping loci associated with milling yield in wheat (Triticum aestivum L.) , 1999, Molecular Breeding.

[7]  M. Soller,et al.  Selective genotyping for determination of linkage between a marker locus and a quantitative trait locus , 1992, Theoretical and Applied Genetics.

[8]  C. Baril Factor regression for interpreting genotype-environment interaction in bread-wheat trials , 1992, Theoretical and Applied Genetics.

[9]  M. Ganal,et al.  A microsatellite map of wheat. , 1998, Genetics.

[10]  J. Nelson QGENE: software for marker-based genomic analysis and breeding , 1997, Molecular Breeding.

[11]  P. Ranjekar,et al.  Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat , 2001, Theoretical and Applied Genetics.

[12]  M. V. Katti,et al.  Molecular Marker Analysis of Protein Content Using PCR-Based Markers in Wheat , 2001, Biochemical Genetics.

[13]  J. Li,et al.  Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross , 1996, Theoretical and Applied Genetics.

[14]  J. Anderson,et al.  Quantitative trait loci for flour viscosity in winter wheat , 1999 .

[15]  E. Lander,et al.  Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. , 1991, Genetics.

[16]  E. Lander,et al.  Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. , 1989, Genetics.

[17]  A. Sciancalepore,et al.  Quantitative trait loci influencing grain protein content in tetraploid wheats , 1996 .

[18]  P. Ranjekar,et al.  Inheritance and identification of DNA markers associated with yellow berry tolerance in wheat (Triticum aestivum L.) , 2004, Euphytica.

[19]  H. Dhaliwal,et al.  Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTL for grain weight in bread wheat , 2000, Theoretical and Applied Genetics.

[20]  M. Gautier,et al.  Linkage between RFLP markers and genes affecting kernel hardness in wheat , 1996, Theoretical and Applied Genetics.

[21]  G. Halloran Genetic analysis of hexaploid wheat, Triticum aestivum using intervarietal chromosome substitution lines—protein content and grain weight , 2004, Euphytica.

[22]  E. R. Sears Nullisomic-Tetrasomic Combinations in Hexaploid Wheat , 1966 .