Larval Growth and Diapause in a Tropical Moth, Omphisa fuscidentalis Hampson

Abstract The bamboo borer, Omphisa fuscidentalis, is a moth found in northern Thailand, Lao and Myanmar and its larvae feed on the inner pulp of bamboo shoots. In a tropical highland (about 500 m sea level) forest at 19°N near Chiang Mai, Thailand, the larvae feed on at least 5 bamboo species. Nucleotide sequence analysis of the region of mitochondrial cytochrome C oxidase subunit 1 gene amplified by the polymerase chain reaction (PCR) verified that larvae collected from different bamboos belong to the same species. Adults appeared in early August and laid clusters of eggs on the newly grown bamboo shoot. The newly hatched larvae bore a hole in the shoot, enter an internode of the shoot and feed on the inner pulp. After maturation in September, the larvae remain in an internodal cavity of bamboo for up to 9 months, from September to the following June. Number of larval instars was estimated by measuring the width of head capsules remained in internodes of bamboo shoots. The growth curve of the width fitted to Dyar's law and the mature larvae were estimated to be 5th instar. Mature larvae were collected in the field each month and their body weight, head capsule width, protein and fat contents and hemolymph ecdysteroid titer were measured. Body weight continuously decreased during the 9 months whereas head capsule width remained constant. Fat content fluctuated during this period while protein level remained at a similar level until March, after which it significantly increased. During this period, hemolymph ecdysteroid concentrations remained low. Current results show that the bamboo borer larvae enter diapause at the end of feeding period of the fifth (last) larval instar and the larval diapause lasts until June.

[1]  S. Sakurai,et al.  Hemolymph ecdysteroid titer and ecdysteroid-dependent developmental events in the last-larval stadium of the silkworm, Bombyx mori: role of low ecdysteroid titer in larval-pupal metamorphosis and a reappraisal of the head critical period. , 1998, Journal of insect physiology.

[2]  V. Košťál,et al.  Physiology of drought tolerance and cold hardiness of the Mediterranean tiger moth Cymbalophora pudica during summer diapause. , 1998, Journal of insect physiology.

[3]  K. Nishimura,et al.  The Testis Development in 3rd- to 6th-Instar Nymphs of the Cricket, Gryllus bimaculatus , 1997 .

[4]  A. Yamanaka,et al.  Species-Specificity in the Action of Big and Small Prothoracicotropic Hormones (PTTHs) of the Swallowtail Butterflies, Papilio xuthus, P. machaon, P. bianor and P. helenus , 1996 .

[5]  D. Frohlich,et al.  Mitochondrial cytochrome C oxidase subunit I of Manduca sexta and a comparison with other invertebrate genes. , 1996, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[6]  B. Crespi,et al.  Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers , 1994 .

[7]  G. Robinson,et al.  A field guide to the smaller moths of South-East Asia , 1994 .

[8]  Kenneth Helrich,et al.  Official methods of analysis of the Association of Official Analytical Chemists , 1990 .

[9]  D. Denlinger,et al.  Seasonal changes in the photoperiodic response regulating diapause in a tropical beetle, Stenotarsus rotundus , 1988 .

[10]  W. J. Bell,et al.  Seasonal adaptations of insects. , 1987 .

[11]  D. Denlinger Dormancy in tropical insects. , 1986, Annual review of entomology.

[12]  D. Denlinger,et al.  Diapause in a large aggregation of a tropical beetle , 1984 .

[13]  Sidney A. Williams,et al.  Official Methods of Analysis of the Association of Official Analytical Chemists , 1971, Soil Science Society of America Journal.

[14]  G. Chippendale,et al.  Seasonal adaptations of populations of the southwestern corn borer, Diatraea grandiosella, from tropical and temperate regions , 1983 .

[15]  D. Denlinger PUPAL DIAPAUSE IN TROPICAL FLESH FLIES: ENVIRONMENTAL AND ENDOCRINE REGULATION, METABOLIC RATE AND GENETIC SELECTION , 1979 .

[16]  P. Scheltes THE CONDITION OF THE HOST PLANT DURING AESTIVATION‐DIAPAUSE OF THE STALK BORERS CHILO PARTELLUS AND CHILO ORICHALCOCILIELLA (LEPIDOPTERA, PYRALIDAE) IN KENYA , 1978 .

[17]  G. Chippendale,et al.  Endocrine interactions controlling the larval diapause of the southwestern corn borer, Diatraea grandiosella , 1976 .

[18]  J. Proctor,et al.  Tropical Rain Forests of the Far East. , 1977 .

[19]  H. Nijhout A threshold size for metamorphosis in the tobacco hornworm, Manduca sexta (L.). , 1975, The Biological bulletin.

[20]  G. Chippendale,et al.  Juvenile hormone regulation of the larval diapause of the Southwestern corn borer, Diatraea grandiosella☆ , 1973 .

[21]  E. J. Usua INDUCTION OF DIAPAUSE IN THE MAIZE STEMBORER, BUSSEOLA FUSCA , 1973 .

[22]  E. J. Usua INDUCTION OF DIAPAUSE IN THE MAIZE STEMBORER , 1973 .

[23]  E. J. Usua Diapause In the Maize Stemborer , 1970 .

[24]  B. Bowers,et al.  PHYSIOLOGY OF INSECT DIAPAUSE. XIII. DNA SYNTHESIS DURING THE METAMORPHOSIS OF THE CECROPIA SILKWORM , 1964 .