Immunochemical localization of extracellular materials in bone marrow of rats

The distribution of type I collagen, fibronectin, laminin, and heparan sulfate was studied in marrow of rats by indirect immunofluorescence. Most of the type I collagen of marrow is associated with large blood vessels and connective tissue trabeculae, but type I collagen was also localized in a delicate meshwork throughout the marrow and in the basement membrane of the sinusoidal endothelium. Fibronectin is partially co‐distributed with type I collagen, but is much more widely distributed. Sheets or septa of fibronectin‐rich material divide the marrow into small compartments that contain and appear to separate clusters of developing blood cells. These septa may serve as a substrate for anchorage and migration of blood cells. Labeling of laminin was observed in the basement membranes of blood vessels, of fat cells, and of the sinusoidal wall, but only scattered labeling was seen in other extracellular materials. Heparan sulfate proteoglycan was poorly labeled in the extracellular matrix of marrow.

[1]  C. Little,et al.  Distribution of laminin, collagen type IV, collagen type I, and fibronectin in chicken cardiac jelly/basement membrane , 1989, The Anatomical record.

[2]  N. Dainiak,et al.  Dual role of fibronectin in hematopoietic differentiation. , 1989, Blood.

[3]  M. Gordon,et al.  Heparan sulfate is necessary for adhesive interactions between human early hemopoietic progenitor cells and the extracellular matrix of the marrow microenvironment. , 1988, Leukemia.

[4]  M. Gordon.  EXTRACELLULAR MATRIX OF THE MARROW MICROENVIRONMENT , 1988, British journal of haematology.

[5]  J. Duband,et al.  Cell-adhesion and substrate-adhesion molecules: their instructive roles in neural crest cell migration. , 1988, Development.

[6]  T. Dexter,et al.  Heparan sulphate bound growth factors: a mechanism for stromal cell mediated haemopoiesis , 1988, Nature.

[7]  L. Coulombel,et al.  Lineage- and stage-specific adhesion of human hematopoietic progenitor cells to extracellular matrices from marrow fibroblasts , 1988 .

[8]  J. McDonald.  Extracellular matrix assembly. , 1988, Annual review of cell biology.

[9]  M. Wicha,et al.  Haemonectin, a bone marrow adhesion protein specific for cells of granulocyte lineage , 1987, Nature.

[10]  H. Lodish,et al.  Differential binding of erythroid and myeloid progenitors to fibroblasts and fibronectin , 1987 .

[11]  L. Weiss,et al.  Ultrastructural localization of heparan sulfate and chondroitin sulfates associated with granulopoiesis in embryonic chick bone marrow. , 1987, The American journal of anatomy.

[12]  B. Voss,et al.  Localization of collagen types I, III, IV and V, fibronectin and laminin in human arteries by the indirect immunofluorescence method. , 1986, Pathology, research and practice.

[13]  P. Comoglio,et al.  Fibronectin-plasma membrane interaction in the adhesion of hemopoietic cells , 1986, The Journal of cell biology.

[14]  J. Risteli,et al.  Immunohistochemical study of basement membrane proteins and type III procollagen in myelofibrosis , 1986, British journal of haematology.

[15]  M. Lyon,et al.  Structure and function of heparan sulphate proteoglycans. , 1986, The Biochemical journal.

[16]  J. Singer,et al.  Proteoglycans in human long-term bone marrow cultures: biochemical and ultrastructural analyses. , 1986, Blood.

[17]  H. M. Patt,et al.  Characterization of rabbit stromal fibroblasts derived from red and yellow bone marrow , 1986, The Journal of experimental medicine.

[18]  W. T. Chen,et al.  Cell adhesion and migration in the early vertebrate embryo: location and possible role of the putative fibronectin receptor complex , 1986, The Journal of cell biology.

[19]  L. Kearney,et al.  Colony formation by primitive haemopoietic progenitors in cocultures of bone marrow cells and stromal cells , 1985, British journal of haematology.

[20]  J. Reilly,et al.  Immuno‐enzymatic detection of fibronectin in normal and pathological haematopoietic tissue , 1985, British journal of haematology.

[21]  M. Wicha,et al.  Inhibition of collagen deposition in the extracellular matrix prevents the establishment of a stroma supportive of hematopoiesis in long-term murine bone marrow cultures. , 1985, The Journal of clinical investigation.

[22]  A. Ciechanover,et al.  Mammalian reticulocytes lose adhesion to fibronectin during maturation to erythrocytes. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[23]  F. Bosman,et al.  Basement membranes in cancer. , 1985, Ultrastructural pathology.

[24]  T. Dexter,et al.  Role of the cellular matrix in haemopoiesis. I. Synthesis of glycosaminoglycans by mouse bone marrow cell cultures. , 1983, Journal of cell science.

[25]  K. Zuckerman,et al.  Extracellular matrix production by the adherent cells of long-term murine bone marrow cultures , 1983 .

[26]  M. Takagi,et al.  Glycosaminoglycans in human neutrophils and leukemic myeloblasts: ultrastructural, cytochemical, immunologic, and biochemical characterization. , 1983, Blood.

[27]  K. Hedman,et al.  Structure of the pericellular matrix: Association of heparan and chondroitin sulfates with fibronectin-procollagen fibers , 1982, Cell.

[28]  S. Bentley.  Collagen synthesis by bone marrow stromal cells: a quantitative study , 1982, British journal of haematology.

[29]  R. Parmley,et al.  Ultrastructural cytochemistry and radioautography of complex carbohydrates in heterophil granulocytes from rabbit bone marrow. , 1980, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[30]  R. Webber,et al.  Electrostimulation and morphologic study of the nerves to the bone marrow of the albino rat. , 1975, Acta anatomica.

[31]  W. Calvo.  The innervation of the bone marrow in laboratory animals. , 1968, The American journal of anatomy.