Restriction fragment length polymorphisms in enzymatically amplified ribosomal DNAs of three heterothallic Pythium species

Three heterothallic Pythium species, P. heterothallicum. P. splendens, and P. sylvaticum, were studied by examining restriction fragment length polymorphisms in polymerase chain reaction-amplified nuclear and mitochondrial ribosomal DNAs. Two different sizes of the internal transcribed spacer (ITS) from the nuclear rDNA were observed. The ITS for P. heterothallicum and P. splendens was about 850 base pair (bp) long, and the ITS for P. sylvaticum was about 1,020 bp long. Each length variant showed distinct banding patterns after restriction enzyme digestions []