Bioluminescence imaging of lymphocyte trafficking in vivo.

Lymphocytes are highly mobile cells that travel throughout the body in response to a tremendous variety of stimuli. Revealing lymphocyte trafficking patterns in vivo is necessary for a complete understanding of immune function, as well as cell-cell and cell-tissue interactions in immune development and in response to insult. Although the location of cell populations in various tissues at any given point in time may be revealed by techniques such as flow cytometry and immunofluorescence, these methods are not readily amenable to the assessment of dynamic cell migration patterns in vivo. In the past 5 years, technologies for imaging molecular and cellular changes in living animals have advanced to a point where it is possible to reveal the migratory paths of these vitally important cells. Here, we review one advancement in cellular imaging, in vivo bioluminescence imaging, which addresses the problem of lymphocyte tracking. This imaging strategy has the potential to elucidate the temporal patterns of immune responses and the spatial distribution of lymphocytes within the body.

[1]  J. S. Reynolds,et al.  Imaging of Spontaneous Canine Mammary Tumors Using Fluorescent Contrast Agents , 1999, Photochemistry and photobiology.

[2]  J. Mullins,et al.  Simian immunodeficiency virus DNA vaccine trial in macaques , 1996, Annals of the New York Academy of Sciences.

[3]  C. Contag,et al.  Antigen-specific T cell-mediated gene therapy in collagen-induced arthritis. , 2001, The Journal of clinical investigation.

[4]  M A Viergever,et al.  Improvement of image resolution and quantitative accuracy in clinical Single Photon Emission Computed Tomography. , 2001, Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society.

[5]  A. Welch,et al.  A review of the optical properties of biological tissues , 1990 .

[6]  Dynamic monitoring and quantification of gene expression in single, living cells: a molecular basis for secretory cell heterogeneity. , 1996, Molecular endocrinology.

[7]  M. Braddock,et al.  Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells. , 1995, Journal of cell science.

[8]  Scott E. Fraser,et al.  Applications of In Vivo Molecular Imaging in Biology and Medicine , 2000 .

[9]  J. S. Reynolds,et al.  Fluorescence Lifetime Spectroscopic Imaging with Measurements of Photon Migration a , 1998, Annals of the New York Academy of Sciences.

[10]  S. Cherry,et al.  Imaging of adenoviral-directed herpes simplex virus type 1 thymidine kinase reporter gene expression in mice with radiolabeled ganciclovir. , 1998, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[11]  R. Jain,et al.  Imaging of activated natural killer cells in mice by positron emission tomography: preferential uptake in tumors. , 1993, Cancer research.

[12]  R. Weissleder,et al.  In vivo imaging of tumors with protease-activated near-infrared fluorescent probes , 1999, Nature Biotechnology.

[13]  M E Phelps,et al.  Design features and performance of a PET system for animal research. , 1992, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[14]  A. Khoruts,et al.  Visualizing the generation of memory CD4 T cells in the whole body , 2001, Nature.

[15]  C. Contag,et al.  Visualizing the kinetics of tumor-cell clearance in living animals. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[16]  B. Rice,et al.  In vivo imaging of light-emitting probes. , 2001, Journal of biomedical optics.

[17]  Simon R. Cherry,et al.  In vivo imaging of neuronal activation and plasticity in the rat brain by high resolution positron emission tomography (microPET) , 2000, Nature Biotechnology.

[18]  C. Contag,et al.  Use of reporter genes for optical measurements of neoplastic disease in vivo. , 2000, Neoplasia.

[19]  S. Cherry,et al.  Performance evaluation of microPET: a high-resolution lutetium oxyorthosilicate PET scanner for animal imaging. , 1999, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[20]  Gansow Oa,et al.  Divalent Cobalt as a Label to Study Lymphocyte Distribution Using PET and SPECT , 1998 .

[21]  S. Cherry,et al.  Repetitive, non-invasive imaging of the dopamine D2 receptor as a reporter gene in living animals , 1999, Gene Therapy.

[22]  Y. Ohmiya,et al.  Cloning, sequence analysis, and expression of active Phrixothrix railroad-worms luciferases: relationship between bioluminescence spectra and primary structures. , 1999, Biochemistry.

[23]  C. Contag,et al.  Noninvasive assessment of tumor cell proliferation in animal models. , 1999, Neoplasia.

[24]  R. Day,et al.  Dual-function reporter protein for analysis of gene expression in living cells. , 1998, BioTechniques.

[25]  Masafumi Oshiro,et al.  Visualizing Gene Expression in Living Mammals Using a Bioluminescent Reporter , 1997, Photochemistry and photobiology.

[26]  M. Sadelain,et al.  Rapid selection of antigen-specific T lymphocytes by retroviral transduction. , 2000, Blood.

[27]  C. Contag,et al.  Adoptive Immunotherapy of Experimental Autoimmune Encephalomyelitis Via T Cell Delivery of the IL-12 p40 Subunit1 , 2001, The Journal of Immunology.

[28]  C. Contag,et al.  In utero delivery of adeno-associated viral vectors: intraperitoneal gene transfer produces long-term expression. , 2001, Molecular therapy : the journal of the American Society of Gene Therapy.

[29]  Krzysztof P Bobinski,et al.  Seeing is believing: Non‐invasive, quantitative and repetitive imaging of reporter gene expression in living animals, using positron emission tomography , 2000, Journal of neuroscience research.

[30]  H. Blau,et al.  In vivo monitoring of myoblast transplantation into rat myocardium. , 2001, The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation.

[31]  J. Mullins,et al.  Photonic detection of bacterial pathogens in living hosts , 1995, Molecular microbiology.

[32]  F. Jöbsis.  Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. , 1977, Science.

[33]  S Falkow,et al.  FACS-optimized mutants of the green fluorescent protein (GFP). , 1996, Gene.

[34]  W C Eckelman,et al.  High resolution PET, SPECT and projection imaging in small animals. , 2001, Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society.

[35]  David K. Stevenson,et al.  Bioluminescent indicators in living mammals , 1998, Nature Medicine.

[36]  Philip J. R. Goulder,et al.  Phenotypic Analysis of Antigen-Specific T Lymphocytes , 1996, Science.

[37]  T. Bergman,et al.  Modulation of Virulence Factor Expression by Pathogen Target Cell Contact , 1996, Science.

[38]  S. Cherry,et al.  Quantification of target gene expression by imaging reporter gene expression in living animals , 2000, Nature Medicine.

[39]  Y. Yonekura,et al.  Ultra-high resolution SPECT system using four pinhole collimators for small animal studies. , 1995, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[40]  R. Negrin,et al.  Expansion of cytotoxic CD3+ CD56+ cells from peripheral blood progenitor cells of patients undergoing autologous hematopoietic cell transplantation. , 2001, Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.

[41]  Ralph Weissleder,et al.  Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells , 2000, Nature Biotechnology.

[42]  R. Negrin,et al.  A novel population of expanded human CD3+CD56+ cells derived from T cells with potent in vivo antitumor activity in mice with severe combined immunodeficiency. , 1994, Journal of immunology.

[43]  W. Semmler,et al.  Near-infrared fluorescent dyes for enhanced contrast in optical mammography: phantom experiments. , 2001, Journal of biomedical optics.

[44]  J. Detre,et al.  Optical investigations of physiology: a study of intrinsic and extrinsic biomedical contrast. , 1997, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[45]  S. Achilefu,et al.  Novel fluorescent contrast agents for optical imaging of in vivo tumors based on a receptor-targeted dye-peptide conjugate platform. , 2001, Journal of biomedical optics.

[46]  G. Winter,et al.  Phage antibodies: filamentous phage displaying antibody variable domains , 1990, Nature.

[47]  J. Altman,et al.  Peptide-MHC Class I Tetrameric Complexes Display Exquisite Ligand Specificity1 , 2000, The Journal of Immunology.

[48]  Daniel E. Hall,et al.  Rapid and quantitative assessment of cancer treatment response using in vivo bioluminescence imaging. , 2000, Neoplasia.

[49]  P F Sharp,et al.  In vivo cell tracking by scanning laser ophthalmoscopy: quantification of leukocyte kinetics. , 1998, Investigative ophthalmology & visual science.