Oxidative metabolism in tomato plants subjected to heat stress

Summary Tomato plants (Lycopersicon esculentum L. cultivar Tmknvf2) were grown for 30 d at two temperatures (25°C, optimal temperature, and 35°C) with the objective of determining the effect of heat stress on oxidative metabolism. The leaf concentrations of the antioxidant compounds ascorbate (AsA), dehydroascorbate (DHA), reduced glutathione (GSH), oxidized glutathione (GSSG) and the activities of the enzymes superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), as well as total hydrogen peroxide (H2O2) concentration and shoot dry weight, were determined. High temperature stress caused: (1) decreased shoot weight, (2) accumulation of H2O2, (3) increased SOD activity, (4) decreased activities of CAT, GPX, APX, DHAR, GR (associated with detoxifying H2O2), and (5) increased levels of the antioxidant compounds AsA, DHA, GSSG, and GSH. In addition, our data demonstrated that heat stress occurred in tomato plants at 35°C, and this temperature may have initially inhibited the ascorbate/glutathione cycle and then provoked an oxidative burst, indicated by foliar H2O2 accumulation.

[1]  A. Magalhães,et al.  Chilling-induced changes in membrane fluidity and antioxidant enzyme activities in Coffea arabica L. roots , 1998, Biologia Plantarum.

[2]  S. Schöner,et al.  Protective systems against active oxygen species in spinach: response to cold acclimation in excess light , 1990, Planta.

[3]  S. Sano,et al.  Changes in antioxidative enzymes in cucumber cotyledons during natural senescence: comparison with those during dark‐induced senescence , 2000 .

[4]  J. Ruíz,et al.  Relationship between boron and phenolic metabolism in tobacco leaves , 1998 .

[5]  W. Van Camp,et al.  Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants , 1997, The EMBO journal.

[6]  G. Paliyath,et al.  Changes in Activities of Antioxidant Enzymes and Their Relationship to Genetic and Paclobutrazol-Induced Chilling Tolerance of Maize Seedlings , 1997, Plant physiology.

[7]  R. Hamilton,et al.  Antioxidant enzyme and compound responses to chilling stress and their combining abilities in differentially sensitive maize hybrids , 1997 .

[8]  M. T. Lafuente,et al.  Effect of high temperature conditioning on ethylene, phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase activities in flavedo of chilled Fortune mandarin fruit , 1997 .

[9]  G. Paliyath,et al.  Ultraviolet-B- and Ozone-Induced Biochemical Changes in Antioxidant Enzymes of Arabidopsis thaliana , 1996, Plant physiology.

[10]  R. Allen,et al.  Dissection of Oxidative Stress Tolerance Using Transgenic Plants , 1995, Plant physiology.

[11]  J. Borrego.  Horticultura herbácea especial , 1995 .

[12]  M. Lucas,et al.  Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton , 1994 .

[13]  T. K. Prasad,et al.  Evidence for Chilling-Induced Oxidative Stress in Maize Seedlings and a Regulatory Role for Hydrogen Peroxide. , 1994, The Plant cell.

[14]  M. Walker,et al.  Role of the Ascorbate-Glutathione Antioxidant System in Chilling Resistance of Tomato , 1993 .

[15]  J. G. Scandalios.  Oxygen Stress and Superoxide Dismutases , 1993, Plant physiology.

[16]  T. Oniki,et al.  Regulation of Peroxidase-Dependent Oxidation of Phenolics in the Apoplast of Spinach Leaves by Ascorbate , 1992 .

[17]  Dirk Inzé,et al.  SUPEROXIDE DISMUTASE AND STRESS TOLERANCE , 1992 .

[18]  L. A. Del Río,et al.  Peroxisome proliferation and oxidative stress mediated by activated oxygen species in plant peroxisomes. , 1991, Archives of biochemistry and biophysics.

[19]  S. Long,et al.  Chilling stress and oxygen metabolizing enzymes in Zea mays and Zea diploperennis , 1991 .

[20]  L. A. Del Río,et al.  Nutritional effect and expression of SODs: induction and gene expression; diagnostics; prospective protection against oxygen toxicity. , 1991, Free radical research communications.

[21]  M. D. De Biasi,et al.  Soluble peroxidase from winter wheat seedlings with phenoloxidase-like activity. , 1990, Plant physiology.

[22]  John F. Ward,et al.  Oxygen Radicals in Biology and Medicine , 1988, Basic Life Sciences.

[23]  I. Fridovich,et al.  Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. , 1987, Analytical biochemistry.

[24]  K. Asada,et al.  Production and scavenging of active oxygen in photosynthesis , 1987 .

[25]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[26]  C. Frenkél,et al.  Involvement of hydrogen peroxide in the regulation of senescence in pear. , 1977, Plant physiology.

[27]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[28]  I. Fridovich,et al.  Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). , 1969, The Journal of biological chemistry.

[29]  P. Urone,et al.  Separation of Hydrogen Peroxide from Organic Hydroperoxides , 1953 .