Analysis of osteoblast-like MG63 cells' response to a rough implant surface by means of DNA microarray.

Several features of the implant surface, such as composition, topography, roughness, and energy, play a relevant role in implant integration with bone. Little is known about the structural and chemical surface properties that may influence biological responses. Expression profiling by DNA microarray is a molecular technology that allows the analysis of gene expression in a cell system. By using DNA microarrays containing 19200 genes, we identified several genes whose expression was significantly down-regulated in osteoblast-like cell line MG63 on a new implant surface (titanium pull spray superficial [TPSS] surface, Oralplant, Cordenons, PN, Italy). The differentially expressed genes cover a broad range of functional activities: (1) signaling transduction, (2) translation, (3) cell cycle regulation, (4) structural and metabolic functions, and (5) apoptosis. It was also possible to detect some genes whose functions are unknown. The data reported can be relevant to better understand the role of the type of surface on the molecular mechanism of implant osseointegration and as a model for comparing other materials.

[1]  S. Volinia,et al.  Genetic portrait of malignant granular cell odontogenic tumour. , 2003, Oral oncology.

[2]  G. Blobel,et al.  Role of Nucleoporin Induction in Releasing an mRNA Nuclear Export Block , 2002, Science.

[3]  S. Volinia,et al.  Identification of differentially expressed genes in human salivary gland tumors by DNA microarrays. , 2002, Molecular cancer therapeutics.

[4]  J. Davies,et al.  Red blood cell and platelet interactions with titanium implant surfaces. , 2000, Clinical oral implants research.

[5]  K Gotfredsen,et al.  Anchorage of titanium implants with different surface characteristics: an experimental study in rabbits. , 2000, Clinical implant dentistry and related research.

[6]  D. Bar-Sagi,et al.  Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. , 2000, Science.

[7]  J. Wroblewski,et al.  Effects of titanium surfaces blasted with TiO2 particles on the initial attachment of cells derived from human mandibular bone. A scanning electron microscopic and histomorphometric analysis. , 2000, Clinical oral implants research.

[8]  J. Scott,et al.  mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes. , 1999, Journal of cell science.

[9]  L. Cooper,et al.  Formation of mineralizing osteoblast cultures on machined, titanium oxide grit-blasted, and plasma-sprayed titanium surfaces. , 1999, The International journal of oral & maxillofacial implants.

[10]  A Wennerberg,et al.  Attachment and proliferation of human oral fibroblasts to titanium surfaces blasted with TiO2 particles. A scanning electron microscopic and histomorphometric analysis. , 1998, Clinical oral implants research.

[11]  R. Nishimura,et al.  Osseointegration enhanced by chemical etching of the titanium surface. A torque removal study in the rabbit. , 1997, Clinical oral implants research.

[12]  E. Wilson,et al.  Identification of a family of low-affinity insulin-like growth factor binding proteins (IGFBPs): characterization of connective tissue growth factor as a member of the IGFBP superfamily. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[13]  D. Brunette,et al.  The effects of micromachined surfaces on formation of bonelike tissue on subcutaneous implants as assessed by radiography and computer image processing. , 1997, Journal of biomedical materials research.

[14]  H. Werner,et al.  Wild-type and mutant p53 differentially regulate transcription of the insulin-like growth factor I receptor gene. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[15]  B. Kasemo,et al.  Bone response to surface-modified titanium implants: studies on the early tissue response to machined and electropolished implants with different oxide thicknesses. , 1996, Biomaterials.

[16]  T. Albrektsson,et al.  Torque and histomorphometric evaluation of c.p. titanium screws blasted with 25- and 75-microns-sized particles of Al2O3. , 1996, Journal of biomedical materials research.

[17]  J. Y. Martin,et al.  Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation. , 1996, Journal of biomedical materials research.

[18]  T Albrektsson,et al.  Experimental study of turned and grit-blasted screw-shaped implants with special emphasis on effects of blasting material and surface topography. , 1996, Biomaterials.

[19]  S. Hammond,et al.  Human ADP-ribosylation Factor-activated Phosphatidylcholine-specific Phospholipase D Defines a New and Highly Conserved Gene Family (*) , 1995, The Journal of Biological Chemistry.

[20]  L. Skovgaard,et al.  Anchorage of TiO2-blasted, HA-coated, and machined implants: an experimental study with rabbits. , 1995, Journal of biomedical materials research.

[21]  D. Davy,et al.  The influence of surface-blasting on the incorporation of titanium-alloy implants in a rabbit intramedullary model. , 1995, The Journal of bone and joint surgery. American volume.

[22]  T. Albrektsson,et al.  An animal study of c.p. titanium screws with different surface topographies , 1995 .

[23]  J. Jansen,et al.  Effect of parallel surface microgrooves and surface energy on cell growth. , 1995, Journal of biomedical materials research.

[24]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[25]  J C Keller,et al.  Optimization of surface micromorphology for enhanced osteoblast responses in vitro. , 1993, The International journal of oral & maxillofacial implants.

[26]  G Weber,et al.  Two distinct cDNAs for human IMP dehydrogenase. , 1990, The Journal of biological chemistry.