A SIX1 Homolog in Fusarium oxysporum f. sp. conglutinans Is Required for Full Virulence on Cabbage

Fusarium oxysporum is a soil-born fungus that induces wilt and root rot on a variety of plants. F. oxysporum f. sp. conglutinans (Foc) can cause wilt disease on cabbage. This study showed that a homolog of SIX1 protein in the Arabidopsis infecting isolate Fo5176 (Fo5176-SIX1) had four isoforms in the conidia of Foc by proteomic analysis. Thus, we analyzed the roles of protein Foc-SIX1. Gene expression analysis showed that, compared to the expression in mycelia, dramatically altered expression of Foc-SIX1 could be detected after infecting cabbages, and Foc-SIX1 was highly expressed in conidia under axenic culture condition. Furthermore, we knocked out the Foc-SIX1 gene and found that Foc-ΔSIX1 mutants had significantly reduced virulence compared with wild type isolate, and full virulence was restored by complementation of Foc-ΔSIX1 mutants with Foc-SIX1. Thus, we concluded that SIX1 in Foc was required for full virulence on cabbage. We also complemented Foc-ΔSIX1 with SIX1 gene in F. oxysporum f. sp. lycopersici (Fol) and found Foc-ΔSIX1::Fol-SIX1 mutants did not affect the virulence of Foc-ΔSIX1. The results confirmed that Fol-SIX1 was not capable of replacing the role of Foc-SIX1 in Foc on the disease symptom development of cabbage. The roles of Fol-SIX1 on virulence might rely on host specificity.

[1]  B. Xie,et al.  Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp. conglutinans that Differ in Pathogenicity , 2015, Scientific Reports.

[2]  G. Wang,et al.  Microscopic analysis of the compatible and incompatible interactions between Fusarium oxysporum f. sp. conglutinans and cabbage , 2015, European Journal of Plant Pathology.

[3]  B. Xie,et al.  Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20. , 2014, FEMS microbiology letters.

[4]  M. Rep,et al.  The Fusarium oxysporum effector Six6 contributes to virulence and suppresses I-2-mediated cell death. , 2014, Molecular plant-microbe interactions : MPMI.

[5]  E. Fukai,et al.  Map-based cloning of a candidate gene conferring Fusarium yellows resistance in Brassica oleracea , 2014, Theoretical and Applied Genetics.

[6]  Xiaowu Wang,et al.  Mapping and analysis of a novel candidate Fusarium wilt resistance gene FOC1 in Brassica oleracea , 2014, BMC Genomics.

[7]  J. Manners,et al.  A highly conserved effector in Fusarium oxysporum is required for full virulence on Arabidopsis. , 2012, Molecular plant-microbe interactions : MPMI.

[8]  Young-Su Seo,et al.  A Phenome-Based Functional Analysis of Transcription Factors in the Cereal Head Blight Fungus, Fusarium graminearum , 2011, PLoS pathogens.

[9]  Zhang Yang-yong.  Research on Screening of Resistant Resources to Fusarium Wilt and Inheritance of the Resistant Gene in Cabbage , 2011 .

[10]  M. Rep,et al.  The arms race between tomato and Fusarium oxysporum. , 2010, Molecular plant pathology.

[11]  Christina A. Cuomo,et al.  Source (or Part of the following Source): Type Article Title Comparative Genomics Reveals Mobile Pathogenicity Chromosomes in Fusarium Author(s) , 2022 .

[12]  M. Rep,et al.  The effector protein Avr2 of the xylem-colonizing fungus Fusarium oxysporum activates the tomato resistance protein I-2 intracellularly. , 2009, The Plant journal : for cell and molecular biology.

[13]  E. Manders,et al.  Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells. , 2008, Fungal genetics and biology : FG & B.

[14]  M. Rep,et al.  Suppression of Plant Resistance Gene-Based Immunity by a Fungal Effector , 2008, PLoS pathogens.

[15]  X. Deng,et al.  A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea , 2007, Electrophoresis.

[16]  Pierre Bonnet,et al.  Intercellular communication in plants: evidence for two rapidly transmitted systemic signals generated in response to electromagnetic field stimulation in tomato. , 2007, Plant, cell & environment.

[17]  B. Wickes,et al.  Split marker transformation increases homologous integration frequency in Cryptococcus neoformans. , 2006, Fungal genetics and biology : FG & B.

[18]  M. Rep.  Small proteins of plant-pathogenic fungi secreted during host colonization. , 2005, FEMS microbiology letters.

[19]  M. Rep,et al.  Fusarium oxysporum evades I-3-mediated resistance without altering the matching avirulence gene. , 2005, Molecular plant-microbe interactions : MPMI.

[20]  C. Scazzocchio,et al.  Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. , 2004, Fungal genetics and biology : FG & B.

[21]  C. D. de Koster,et al.  A small, cysteine‐rich protein secreted by Fusarium oxysporum during colonization of xylem vessels is required for I‐3‐mediated resistance in tomato , 2004, Molecular microbiology.

[22]  Natalie L. Catlett,et al.  Split-Marker Recombination for Efficient Targeted Deletion of Fungal Genes , 2003 .

[23]  T. Kuang,et al.  Proteomics approach to identify wound‐response related proteins from rice leaf sheath , 2003, Proteomics.

[24]  C. D. de Koster,et al.  Mass Spectrometric Identification of Isoforms of PR Proteins in Xylem Sap of Fungus-Infected Tomato1 , 2002, Plant Physiology.

[25]  N. Talbot,et al.  Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. , 1993, The Plant cell.

[26]  B. Turgeon,et al.  Development of a fungal transformation system based on selection of sequences with promoter activity , 1987, Molecular and cellular biology.

[27]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.