Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus

We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 102 TCID50/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 105 TCID50/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 103 to 105 TCID50/mL.

[1]  Gil U. Lee,et al.  A biosensor based on magnetoresistance technology. , 1998, Biosensors & bioelectronics.

[2]  Tianhong Cui,et al.  Carbon nanotube electric immunoassay for the detection of swine influenza virus H1N1 , 2011, Biosensors and Bioelectronics.

[3]  Jian-Ping Wang,et al.  A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine. , 2009, Angewandte Chemie.

[4]  Jianping Wang,et al.  Surface Modification for Protein and DNA Immobilization onto GMR Biosensor , 2013, IEEE Transactions on Magnetics.

[5]  P. Messersmith,et al.  Multifunctional Magnetic Nanochains: Exploiting Self-Polymerization and Versatile Reactivity of Mussel-Inspired Polydopamine , 2015 .

[6]  Shigui Yang,et al.  Simultaneous Detection of Influenza A, Influenza B, and Respiratory Syncytial Viruses and Subtyping of Influenza A H3N2 Virus and H1N1 (2009) Virus by Multiplex Real-Time PCR , 2011, Journal of Clinical Microbiology.

[7]  Magnetic particle-based ultrasensitive biosensors for , 2012 .

[8]  Binasch,et al.  Enhanced magnetoresistance in layered magnetic structures with antiferromagnetic interlayer exchange. , 1989, Physical review. B, Condensed matter.

[9]  J Loureiro,et al.  Magnetoresistive chip cytometer. , 2011, Lab on a chip.

[10]  D. Hughes,et al.  Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites. , 2014, Virology.

[11]  I-Ming Hsing,et al.  Micro‐ and Nano‐ Magnetic Particles for Applications in Biosensing , 2007 .

[12]  A. Pühler,et al.  Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. , 2004, Biosensors & bioelectronics.

[13]  Barjor Gimi,et al.  Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion. , 2013, Biosensors & bioelectronics.

[14]  Y. Mugnier,et al.  Individual inorganic nanoparticles: preparation, functionalization and in vitro biomedical diagnostic applications. , 2013, Journal of materials chemistry. B.

[15]  Etienne,et al.  Giant magnetoresistance of (001)Fe/(001)Cr magnetic superlattices. , 1988, Physical review letters.

[16]  A. Vincent,et al.  Novel Swine Influenza Virus Subtype H3N1, United States , 2006, Emerging infectious diseases.

[17]  James A. Smagala,et al.  Experimental Evaluation of the FluChip Diagnostic Microarray for Influenza Virus Surveillance , 2006, Journal of Clinical Microbiology.

[18]  Claude Fermon,et al.  Toward a magnetoresistive chip cytometer: Integrated detection of magnetic beads flowing at cm/s velocities in microfluidic channels , 2009 .

[19]  Yi Wang,et al.  Giant magnetoresistive-based biosensing probe station system for multiplex protein assays. , 2015, Biosensors & bioelectronics.

[20]  Jaesung Jang,et al.  Electrical immunosensor based on dielectrophoretically-deposited carbon nanotubes for detection of influenza virus H1N1. , 2014, The Analyst.

[21]  Gengfeng Zheng,et al.  Electrical detection of single viruses. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Daniel L Graham,et al.  Magnetoresistive-based biosensors and biochips. , 2004, Trends in biotechnology.

[23]  Jian-Ping Wang,et al.  A three-layer competition-based giant magnetoresistive assay for direct quantification of endoglin from human urine. , 2011, Analytical chemistry.

[24]  M. Bangar,et al.  Label-free chemiresistive immunosensors for viruses. , 2010, Environmental science & technology.

[25]  J. C. Rifea,et al.  Design and performance of GMR sensors for the detection of magnetic microbeads in biosensors , 2003 .

[26]  Anh-Tuan Le,et al.  DNA sensor development based on multi-wall carbon nanotubes for label-free influenza virus (type A) detection. , 2009, Journal of immunological methods.

[27]  Yong Poovorawan,et al.  Single step multiplex real-time RT-PCR for H5N1 influenza A virus detection. , 2006, Journal of virological methods.

[28]  E. Thacker,et al.  Comparison of Three Serological Assays to Determine the Cross-Reactivity of Antibodies from Eight Genetically Diverse U.S. Swine Influenza Viruses , 2008, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[29]  Yi Wang,et al.  Magnetoresistive performance and comparison of supermagnetic nanoparticles on giant magnetoresistive sensor-based detection system , 2014, Scientific Reports.

[30]  R. Webster,et al.  Potential for transmission of avian influenza viruses to pigs. , 1994, The Journal of general virology.

[31]  M. Zambon,et al.  Molecular diagnosis of influenza , 2002, Reviews in medical virology.

[32]  Xiao Zhi,et al.  Quick genotyping detection of HBV by giant magnetoresistive biochip combined with PCR and line probe assay. , 2012, Lab on a chip.

[33]  Quan Cheng,et al.  Detection of influenza virus: traditional approaches and development of biosensors , 2005, Analytical and bioanalytical chemistry.

[34]  Marc D Porter,et al.  Giant magnetoresistive sensors and superparamagnetic nanoparticles: a chip-scale detection strategy for immunosorbent assays. , 2005, Analytical chemistry.

[35]  R. Tripp,et al.  One-step assay for detecting influenza virus using dynamic light scattering and gold nanoparticles. , 2011, The Analyst.

[36]  Nader Pourmand,et al.  Giant magnetoresistive biochip for DNA detection and HPV genotyping. , 2008, Biosensors & bioelectronics.

[37]  Dejian Zhou,et al.  Magnetic particle-based ultrasensitive biosensors for diagnostics , 2012, Expert review of molecular diagnostics.

[38]  Daniel Kappe,et al.  Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface , 2013, Biosensors.

[39]  E. Król,et al.  Universal biosensor for detection of influenza virus. , 2014, Biosensors & bioelectronics.

[40]  Ronghui Wang,et al.  A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1. , 2011, Biosensors & bioelectronics.

[41]  Drew A. Hall,et al.  Quantification of Protein Interactions and Solution Transport Using High-Density GMR Sensor Arrays , 2011, Nature nanotechnology.

[42]  R F Bey,et al.  ELISA Method for Detection of Influenza A Infection in Swine , 1993, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.