This investigation was conducted to determine the kinematic response of the lumbar spine instrumented with transpedicular screws and plates. Seven unembalmed human cadaveric lumbar spines were used. Retroreflective targets were inserted into the bony landmarks of each vertebral body, facet column, and spinous process. The specimen was quasistatically loaded until failure (initial cycle) using an electrohydraulic testing device at a rate of 2.5 mm/sec. After radiography, the specimen was again loaded (injury cycle) to the failure compression determined in the previous cycle. Transpedicular screws then were inserted bilaterally at one level proximal and distal to injury. The stabilized cycle of loading was conducted using the procedure adopted in the injury cycle. Comparative analysis of the localized kinematic data between the stabilized and injured columns indicated a reduction in motion between fixated levels, increasing the rigidity of the column. At levels proximal and distal to fixation, however, motion increased, indicating added flexibility. These alterations in the motion, observed during single-cycle loading, may be further accentuated in vivo, leading to hypermobility and degeneration of the spine.