Study on Extraction of Porphyra Polysaccahride and its Antioxidant Properties

On the base of single factor including extraction temperature, extraction time and the ratio of raw materials to water, experiments the research aims to explore the suitable consideration considering the optimal conditions of extracting porphyra polysaccharide. According to the experiments, the optimal conditions for extracting porphyra polysaccharide can be as follows: extraction temperature 80 °C, extraction time 2 h, the ratio of raw materials to water was 1: 20. Following this, the yield of porphyra polysaccharide could be 3 %. On the next stage, Sevag has been employed to remove the obtained protein. After six times, the content of protein can be reduced from 14. 31 % to 6. 0 % whilst the content of porphyra polysaccharide can reach 87. 66 %. The result of antioxidant activity revealed that the extract showed good scavenging effect for·OH and DPPH·. Besides, there were obvious positive relationship between the concentration and activity of the extract. When the concentration of porphyra polysaccharide was 3 mg/mL, ·OH scavenging rate can reach 90. 9 %; and when the concentration of porphyra polysaccharide was 2. 5 mg/mL, DPPH·scavenging rate can reach 90 %.

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