DEVELOPMENT AND RECOVERY OF LASER-INDUCED RETINAL LESION IN RATS

Purpose: Laser-induced retinal lesions undergo primary and secondary degeneration followed by a partial reduction of the lesion size. To evaluate treatment effects, detailed data regarding the changes of the lesion over time are essential. The purpose of the study is to describe the histologic changes in an argon laser-induced retinal lesion over a period of 60 days. Methods: Argon laser lesions were produced in retinas of pigmented rats. The lesions were examined by light microscopy 1 hour and 1, 2, 3, 20, and 60 days after the exposure. Results: The diameter of the lesion increased 24 hours after photocoagulation and then decreased by day 20. Most pyknotic nuclei seen in the outer nuclear layer 1 hour after lasering disappeared 3 days later. Remodeling began 3 days after lasering. By day 60, partial filling in of the empty area with sliding of adjacent nuclei was observed. Recovery was also seen in the other retinal layers. Conclusion: The course of a laser-induced retinal lesion is gradual: the photoreceptors are damaged first and the damage then spreads to other layers and to the adjacent retina. By day 3, the damage spreading stops, and adjacent cells begin to fill in and remodel the area of the lesion.

[1]  M. Tso,et al.  Methylprednisolone therapy in laser injury of the retina , 1993, Graefe's Archive for Clinical and Experimental Ophthalmology.

[2]  N. Bressler,et al.  Photodynamic therapy of subfoveal choroidal neovascularization in age-related macular degeneration with verteporfin: one-year results of 2 randomized clinical trials--TAP report. Treatment of age-related macular degeneration with photodynamic therapy (TAP) Study Group. , 1999, Archives of ophthalmology.

[3]  D. van Norren,et al.  Filling-in after focal loss of photoreceptors in rat retina. , 1999, Experimental eye research.

[4]  R. Brancato,et al.  Photocoagulation scar expansion after laser therapy for choroidal neovascularization in degenerative myopia. , 1990, Retina.

[5]  D. Norren,et al.  Temporal sequence of changes in rat retina after UV-A and blue light exposure , 1999, Vision Research.

[6]  M. Tso,et al.  The effect of high-dose methylprednisolone on laser-induced retinal injury in primates: an electron microscopic study , 1997, Graefe's Archive for Clinical and Experimental Ophthalmology.

[7]  Y. Epstein,et al.  Functional deficits resulting from laser‐induced damage in the rat retina , 2006, Lasers in surgery and medicine.

[8]  G O Waring,et al.  Comparison of laser phacovaporization using the Er-YAG and the Er-YSGG laser. , 1993, Archives of ophthalmology.

[9]  T. Kuwabara,et al.  The effects of laser photocoagulation in the retinal capillaries. , 1975, American journal of ophthalmology.

[10]  Michael Stur,et al.  Acute severe visual acuity decrease after photodynamic therapy with verteporfin: case reports from randomized clinical trials-TAP and VIP report no. 3. , 2004, American journal of ophthalmology.

[11]  Michael Belkin,et al.  Neuroprotective therapy for argon-laser-induced retinal injury , 1999, Photonics West - Biomedical Optics.

[12]  M Flocks,et al.  Retinal injury due to industrial laser burns. , 1981, Ophthalmology.

[13]  R Birngruber,et al.  Clinical observations of six cases of laser injury to the eye. , 1989, Health physics.

[14]  J. Jonas.  VERTEPORFIN THERAPY OF SUBFOVEAL CHOROIDAL NEOVASCULARIZATION IN AGE-RELATED MACULAR DEGENERATION: Meta-Analysis of 2-Year Safety Results in Three Randomized Clinical Trials: Treatment of Age-Related Macular Degeneration with Photodynamic Therapy and Verteporfin in Photodynamic Therapy Study Report , 2002, American journal of ophthalmology.

[15]  M. Belkin,et al.  Methylprednisolone therapy for retinal laser injury. , 1999, Survey of ophthalmology.

[16]  Atul K. Jain,et al.  Healing of retinal photocoagulation lesions. , 2008, Investigative ophthalmology & visual science.

[17]  Xu Jm,et al.  Ocular Injuries from Accidental Laser Exposure , 1989 .

[18]  M. Goldberg,et al.  Retinal damage from a Q-switched YAG laser. , 1983, American journal of ophthalmology.

[19]  R. Collier,et al.  Temporal sequence of changes to the gray squirrel retina after near-UV exposure. , 1989, Investigative ophthalmology & visual science.

[20]  Poon Am,et al.  The effect of low dose laser on the functional activities of the mice retina. , 1980 .

[21]  M. Belkin,et al.  Laser eye injuries. , 2000, Survey of ophthalmology.

[22]  J. Mellerio,et al.  Histology of the formation of retinal laser lesions. , 1967, Experimental eye research.

[23]  A. R. Pilkerton,et al.  A corneal complication of indirect ophthalmic laser delivery systems. , 1990, American journal of ophthalmology.

[24]  M. Landers,et al.  Comparison of photocoagulation with the argon, krypton, and diode laser indirect ophthalmoscopes in rabbit eyes. , 1992, Ophthalmology.

[25]  Julian D. C. Jones,et al.  Handbook of Laser Technology and Applications , 2004 .

[26]  J. M. Risco,et al.  Choroidal microvascular repair after argon laser photocoagulation. , 1982, American journal of ophthalmology.

[27]  J. Souchek,et al.  Reproducibility and validity of laser flare/cell meter measurements as an objective method of assessing intraocular inflammation. , 1992, Archives of ophthalmology.

[28]  K. Kratz,et al.  Changes in ERG amplitude following laser induced damage to the primate retina. , 1990, Current eye research.

[29]  W. T. Ham,et al.  Action spectrum for retinal injury from near-ultraviolet radiation in the aphakic monkey. , 1982, American journal of ophthalmology.

[30]  W. Smiddy,et al.  Corneal and iris burns with the laser indirect ophthalmoscope. , 1990, American journal of ophthalmology.

[31]  D. C. Wu,et al.  Ocular injuries from accidental laser exposure. , 1989, Health physics.

[32]  Atul K. Jain,et al.  Effect of pulse duration on size and character of the lesion in retinal photocoagulation. , 2008, Archives of ophthalmology.

[33]  A C Bird,et al.  Histopathology of ruby and argon laser lesions in monkey and human retina. A comparative study. , 1975, The British journal of ophthalmology.

[34]  Jeremiah Brown,et al.  Assessment of alleged retinal laser injuries. , 2004, Archives of ophthalmology.

[35]  J. Pulido,et al.  Tissue effects of subclinical diode laser treatment of the retina. , 1998, Archives of ophthalmology.

[36]  Noemi Bitterman,et al.  Development of laser-induced retinal damage in the rabbit , 1999, Graefe's Archive for Clinical and Experimental Ophthalmology.

[37]  Y. Buys,et al.  Glaucoma laser suture lysis. , 1996, The British journal of ophthalmology.

[38]  M. Goldberg,et al.  Histopathology and ultrastructure of the argon laser lesion in human retinal and choroidal vasculatures. , 1973, American journal of ophthalmology.

[39]  Jennifer I. Lim,et al.  Verteporfin therapy of subfoveal choroidal neovascularization in age-related macular degeneration: meta-analysis of 2-year safety results in three randomized clinical trials: Treatment Of Age-Related Macular Degeneration With Photodynamic Therapy and Verteporfin In Photodynamic Therapy Study Report , 2004, Retina.

[40]  T. Lai,et al.  Transient reduction in retinal function revealed by multifocal electroretinogram after photodynamic therapy. , 2004, American journal of ophthalmology.

[41]  Y. Ogura,et al.  Alterations of retinal microcirculation in response to scatter photocoagulation. , 1998, Investigative ophthalmology & visual science.

[42]  S. Kishi,et al.  PROGRESSIVE ENLARGEMENT OF SCATTERED PHOTOCOAGULATION SCARS IN DIABETIC RETINOPATHY , 2004, Retina.

[43]  D J D'Amico,et al.  Multicenter clinical experience using an erbium:YAG laser for vitreoretinal surgery. , 1996, Ophthalmology.

[44]  B. S. Fine,et al.  Repair and late degeneration of the primate foveola after injury by argon laser. , 1979, Investigative ophthalmology & visual science.

[45]  T. Weingeist.  Argon laser photocoagulation of the human retina. I. Histopathologic correlation of chorioretinal lesions in the region of the maculopapillar bundle. , 1974, Investigative ophthalmology.

[46]  A. Ducatman,et al.  Laser hazards in research laboratories. , 1993, Journal of occupational medicine. : official publication of the Industrial Medical Association.