Objective To explore the correlation of two β2-adrenergic receptor (β2-AR)polymorphisms (Arg16Gly,Gln27Glu) with the pathogenesis of myasthenia gravis (MG).Methods The genotypes of β2-AR in 88 MG patients and 91 age and gender-matched healthy controls (HC) were determined by polymerese chain reaction-restriction fragraent length polymorphism (PCR-RFLP) analysis.Results The frequencies of three genotypes (Arg/Arg,Arg/Gly and Gly/Gly) in position 16 were 44.4%,38.6%,17.0% in MG group and 29.7%,58.2%,12.1% in HC group respectively.The difference in distribution of the genotypes between MG group and HC group was statistically significant (X~2=6.898,P=0.032).The frequencies of Arg and Gly allele were 63.6% and 36.4% in MG group,and 58.8% and 41.2% in HC group.The difference in distribution of the alleles between MG group and HC group was statistically insignificant.The frequencies of 3 genotypes (Gln/Gln,Gln/Glu and Glu/Glu) in position 27 were 77.3%,20.4% and 2.3% in MG group and 78.0%,18.7%,3.3% in HC group respectively.The frequencies of Gin and Glu allele were 87.5%,12.5% in MG group,and 87.4%,12.6% in HC group.There was no significant difference in distribution of genotypes or alleles between MG group and HC group.Conclusion β2-AR gene polymorphism in position 16 may independently increase the risk of onset of MG,and there is no correlation between the polymorphism in position 27 of β2-AR and the onset of MG.
Key words:
Myasthenia grads; Receptors; adrenergic; beta-2; Polymorphism; single nucleotide